Overcoming barriers to membrane protein structure determination (Q30401692)

21 June 2017

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title

Overcoming barriers to membrane protein structure determination (English)

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protein structure determination

21 June 2017

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7

Simon Newstead

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21 June 2017

2

Peter J F Henderson

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21 June 2017

Christopher G Tate

9

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Roslyn M. Bill

object named as

Roslyn M Bill

1

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Hartmut Michel

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object named as

Hartmut Michel

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21 June 2017

6

Richard Neutze

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21 June 2017

3

So Iwata

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21 June 2017

Edmund R S Kunji

4

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PubMed ID

21478852

21 June 2017

Bert Poolman

8

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PubMed ID

21478852

21 June 2017

Horst Vogel

10

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21 June 2017

1 April 2011

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21 June 2017

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21 June 2017

29

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21 June 2017

335-340

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21 June 2017

4

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Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy

21 June 2017

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High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor

21 January 2018

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Crystal structure of squid rhodopsin

21 January 2018

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Crystal structure of the ligand-free G-protein-coupled receptor opsin

21 January 2018

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Crystal structure of the human beta2 adrenergic G-protein-coupled receptor

21 January 2018

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GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function

21 January 2018

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Crystal structure of opsin in its G-protein-interacting conformation

21 January 2018

1 reference

The structural basis of calcium transport by the calcium pump

21 January 2018

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Crystal structure of the sodium-potassium pump at 2.4 A resolution

21 January 2018

1 reference

The Crystal Structure of a Sodium Galactose Transporter Reveals Mechanistic Insights into Na+/Sugar Symport

21 January 2018

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Structure and mechanism of an amino acid antiporter

21 January 2018

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Molecular basis of transport and regulation in the Na(+)/betaine symporter BetP

21 January 2018

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Structure and Mechanism of a Na+-Independent Amino Acid Transporter

21 January 2018

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Molecular Basis of Alternating Access Membrane Transport by the Sodium-Hydantoin Transporter Mhp1

21 January 2018

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The alternating access mechanism of transport as observed in the sodium-hydantoin transporter Mhp1.

21 January 2018

1 reference

Structure and Molecular Mechanism of a Nucleobase-Cation-Symport-1 Family Transporter

21 January 2018

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Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding

21 January 2018

1 reference

Structural basis of trans-inhibition in a molybdate/tungstate ABC transporter

21 January 2018

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Structure of a potentially open state of a proton-activated pentameric ligand-gated ion channel

21 January 2018

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Making membrane proteins for structures: a trillion tiny tweaks

21 January 2018

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Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

21 January 2018

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Tuning Escherichia coli for membrane protein overexpression

21 January 2018

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Overexpression of integral membrane proteins for structural studies

21 January 2018

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Overexpression of mammalian integral membrane proteins for structural studies.

21 January 2018

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Purification of a rat neurotensin receptor expressed in Escherichia coli

21 January 2018

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Export from the endoplasmic reticulum represents the limiting step in the maturation and cell surface expression of the human delta opioid receptor

21 January 2018

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Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification.

21 January 2018

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Molecular chaperones stimulate the functional expression of the cocaine-sensitive serotonin transporter.

21 January 2018

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Calnexin co-expression and the use of weaker promoters increase the expression of correctly assembled Shaker potassium channel in insect cells

21 January 2018

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Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.

21 January 2018

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Consequences of membrane protein overexpression in Escherichia coli.

21 January 2018

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Design of improved membrane protein production experiments: quantitation of the host response

21 January 2018

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Relieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein production.

21 January 2018

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Genome sequence of the recombinant protein production host Pichia pastoris

21 January 2018

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Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures

21 January 2018

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Stress in recombinant protein producing yeasts.

21 January 2018

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Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory

21 January 2018

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Lactococcus lactis as host for overproduction of functional membrane proteins

21 January 2018

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Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics.

21 January 2018

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Selenomethionine incorporation in proteins expressed inLactococcus lactis

21 January 2018

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Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis

21 January 2018

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The structural basis for peptide selection by the transport receptor OppA

21 January 2018

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Optimization of membrane protein overexpression and purification using GFP fusions.

21 January 2018

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A scalable, GFP-based pipeline for membrane protein overexpression screening and purification

21 January 2018

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Quality control of overexpressed membrane proteins

21 January 2018

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High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae

21 January 2018

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Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins

21 January 2018

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Visualizing odorant receptor trafficking in living cells down to the single-molecule level

21 January 2018

1 reference

FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells

21 January 2018

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Probing the function of ionotropic and G protein-coupled receptors in surface-confined membranes.

21 January 2018

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Engineering G protein-coupled receptors to facilitate their structure determination.

21 January 2018

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Crystal structure of rhodopsin: A G protein-coupled receptor

21 January 2018

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Crystallizing membrane proteins using lipidic mesophases

21 January 2018

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Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli

21 January 2018

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The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist

21 January 2018

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Determination of the molecular mass and dimensions of membrane proteins by size exclusion chromatography

21 January 2018

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Building a thermostable membrane protein

21 January 2018

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Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor

21 January 2018

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Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form

21 January 2018

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Thermostabilization of the neurotensin receptor NTS1.

21 January 2018

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Development and crystallization of a minimal thermostabilised G protein-coupled receptor.

21 January 2018

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A Specific Cholesterol Binding Site Is Established by the 2.8 Å Structure of the Human β2-Adrenergic Receptor

21 January 2018

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Conserved Binding Mode of Human β 2 Adrenergic Receptor Inverse Agonists and Antagonist Revealed by X-ray Crystallography

21 January 2018

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Structure of a beta1-adrenergic G-protein-coupled receptor

21 January 2018

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Structures of the CXCR4 chemokine GPCR with small-molecule and cyclic peptide antagonists

21 January 2018

1 reference

Structure of the Human Dopamine D3 Receptor in Complex with a D2/D3 Selective Antagonist

21 January 2018

1 reference

Protein conformational changes in the bacteriorhodopsin photocycle: comparison of findings from electron and X-ray crystallographic analyses.

21 January 2018

1 reference

The structure of the aquaporin-1 water channel: a comparison between cryo-electron microscopy and X-ray crystallography

21 January 2018

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Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy

21 January 2018

1 reference

Bacteriorhodopsin: a high-resolution structural view of vectorial proton transport

21 January 2018

1 reference

Crystal structure of a mammalian voltage-dependent Shaker family K+ channel

21 January 2018

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Diffraction cartography: applying microbeams to macromolecular crystallography sample evaluation and data collection

21 January 2018

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Protein crystallography with a micrometre-sized synchrotron-radiation beam

21 January 2018

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Potential for biomolecular imaging with femtosecond X-ray pulses

21 January 2018

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Structure at 2.8 A resolution of cytochrome c oxidase from Paracoccus denitrificans

21 January 2018

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Fluorescence Detection of Heavy Atom Labeling (FD-HAL): a rapid method for identifying covalently modified cysteine residues by phasing atoms.

21 January 2018

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Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides

21 January 2018

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Super-resolution biomolecular crystallography with low-resolution data

21 January 2018

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Light-induced structural changes in a photosynthetic reaction center caught by Laue diffraction

21 January 2018

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Structural dynamics of light-driven proton pumps

21 January 2018

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Yeast mitochondrial ADP/ATP carriers are monomeric in detergents

21 January 2018

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Changing single side-chains can greatly enhance the resistance of a membrane protein to irreversible inactivation

21 January 2018

1 reference

12 December 2020

Intracellular turnover of cystic fibrosis transmembrane conductance regulator. Inefficient processing and rapid degradation of wild-type and mutant proteins.

1 reference

12 December 2020

Practical Considerations of Membrane Protein Instability during Purification and Crystallisation

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12 December 2020

Transferability of thermostabilizing mutations between β-adrenergic receptors

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12 December 2020

Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase

1 reference

12 December 2020

Production of membrane proteins in Escherichia coli and Lactococcus lactis

1 reference

12 December 2020

Structure of a prokaryotic virtual proton pump at 3.2 Å resolution

1 reference

12 December 2020

The Nucleobase-Cation-Symport-1 Family of Membrane Transport Proteins

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7 January 2021

inferred from DOI database lookup

Identifiers

DOI

10.1038/NBT.1833

1 reference

21 June 2017

1 reference

PubMed ID

21478852