Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening (Q36274327)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:28178357%20AND%20SRC:MED&resulttype=core&format=json

2 April 2020

title

Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening (English)

1 reference

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2 April 2020

0 references

1 reference

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1

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2 April 2020

Maximilian Ebert

series ordinal

2

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2 April 2020

4

Joelle N Pelletier

1 reference

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2 April 2020

author name string

Paul F Mugford

series ordinal

3

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2 April 2020

language of work or name

English

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publication date

8 February 2017

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2 April 2020

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2 April 2020

volume

12

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2 April 2020

issue

2

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2 April 2020

page(s)

e0171741

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Creative Commons Attribution 4.0 International

2 April 2020

copyright license

start time

8 February 2017

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

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cites work

HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Protein Engineering by Combined Computational and In Vitro Evolution Approaches

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Mutanalyst, an online tool for assessing the mutational spectrum of epPCR libraries with poor sampling

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Methods for the directed evolution of proteins

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Reducing codon redundancy and screening effort of combinatorial protein libraries created by saturation mutagenesis.

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

To get what we aim for--progress in diversity generation methods

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Strategies for the discovery and engineering of enzymes for biocatalysis

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Parallel assembly for multiple site-directed mutagenesis of plasmids

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Directed enzyme evolution: beyond the low-hanging fruit

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Multiple Conformers in Active Site of Human Dihydrofolate Reductase F31R/Q35E Double Mutant Suggest Structural Basis for Methotrexate Resistance

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Advances in generating functional diversity for directed protein evolution.

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

A one pot, one step, precision cloning method with high throughput capability

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

X-ray structure of Candida antarctica lipase A shows a novel lid structure and a likely mode of interfacial activation

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Increasing methotrexate resistance by combination of active-site mutations in human dihydrofolate reductase

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Protein production by auto-induction in high density shaking cultures

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

General method for sequence-independent site-directed chimeragenesis

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Combinatorial and computational challenges for biocatalyst design

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Specific and sensitive plate assay for bacterial lipases

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

A rapid DNA assembling strategy mediated by direct full-length polymerase chain reaction

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Modular construction of plasmids by parallel assembly of linear vector components

22 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Determination of lipase activity by a rhodamine-triglyceride-agarose assay.

22 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5298319

Finding better protein engineering strategies

22 September 2018

1 reference

Crossref

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0171741

Creation of a Lipase Highly Selective for trans Fatty Acids by Protein Engineering

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0171741

The importance of additive and non-additive mutational effects in protein engineering

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/28178357

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1371/JOURNAL.PONE.0171741

1 reference

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2 April 2020

0 references

1 reference

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2 April 2020

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