Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries (Q33283855)

25 July 2017

Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries (English)

1 reference

25 July 2017

Asael Herman

1

1 reference

PubMed ID

17483523

25 July 2017

Dan S Tawfik

series ordinal

2

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25 July 2017

5 May 2007

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Protein Engineering Design and Selection

25 July 2017

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25 July 2017

20

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25 July 2017

219-226

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25 July 2017

5

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High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments.

25 July 2017

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Altering substrate specificity of phosphatidylcholine-preferring phospholipase C of Bacillus cereus by random mutagenesis of the headgroup binding site

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Antibody engineering by parsimonious mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Computational design of a biologically active enzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

RAISE: a simple and novel method of generating random insertion and deletion mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Directed evolution of the promiscuous esterase activity of carbonic anhydrase II.

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Combining computational and experimental screening for rapid optimization of protein properties

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

ConSurf 2005: the projection of evolutionary conservation scores of residues on protein structures

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Active TEM-1 beta-lactamase mutants with random peptides inserted in three contiguous surface loops

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Engineered protein function by selective amino acid diversification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Strategies and computational tools for improving randomized protein libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Active site engineering of the epoxide hydrolase from Agrobacterium radiobacter AD1 to enhance aerobic mineralization of cis-1,2-dichloroethylene in cells expressing an evolved toluene ortho-monooxygenase

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Directed evolution of the site specificity of Cre recombinase

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Low free energy cost of very long loop insertions in proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Semi-synthetic DNA shuffling of aveC leads to improved industrial scale production of doramectin by Streptomyces avermitilis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Man-made cell-like compartments for molecular evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Building novel binding ligands to B7.1 and B7.2 based on human antibody single variable light chain domains

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1093%2FPROTEIN%2FGZM014

21 January 2018

Identifiers

DOI

10.1093/PROTEIN/GZM014

1 reference

25 July 2017

1 reference

PubMed ID

17483523