Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications (Q30414571)

21 June 2017

Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications (English)

1 reference

21 June 2017

Anne S Robinson

3

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author name string

Carissa L Young

series ordinal

1

1 reference

PubMed ID

22442034

21 June 2017

Zachary T Britton

series ordinal

2

1 reference

PubMed ID

22442034

21 June 2017

language of work or name

1 reference

10 January 2012

1 reference

21 June 2017

Biotechnology Journal

1 reference

21 June 2017

7

1 reference

21 June 2017

620-634

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21 June 2017

5

1 reference

Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

21 June 2017

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Making the most of affinity tags

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Tagging recombinant proteins to enhance solubility and aid purification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High-Throughput Protein Production (HTPP): a review of enabling technologies to expedite protein production

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Design of high-throughput methods of protein production for structural biology

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The three-dimensional structure of a glutathione S-transferase from the mu gene class. Structural analysis of the binary complex of isoenzyme 3-3 and glutathione at 2.2-A resolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The dimerization property of glutathione S-transferase partially reactivates Bcr-Abl lacking the oligomerization domain.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Using Schizosaccharomyces pombe as a host for expression and purification of eukaryotic proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Purification of glutathione S-transferase fusion proteins from yeast.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

p107wee1 is a dual-specificity kinase that phosphorylates p34cdc2 on tyrosine 15

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

pLEF, a novel vector for expression of glutathione S-transferase fusion proteins in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The noncatalytic src homology region 2 segment of abl tyrosine kinase binds to tyrosine-phosphorylated cellular proteins with high affinity

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Crystal structures of a schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Two regions of mature periplasmic maltose-binding protein of Escherichia coli involved in secretion

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Properties of soluble fusions between mammalian aspartic proteinases and bacterial maltose-binding protein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Expression and purification of recombinant proteins by fusion to maltose-binding protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Improving solubility of Shewanella oneidensis MR-1 and Clostridium thermocellum JW-20 proteins expressed into Esherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

AMP-activated protein kinase kinase: detection with recombinant AMPK alpha1 subunit

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Purification and characterization of VanXY(C), a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Crystal structure of thioredoxin from Escherichia coli at 1.68 A resolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Thioredoxin as a fusion tag for carrier-driven crystallization

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Modification of nuclear PML protein by SUMO-1 regulates Fas-induced apoptosis in rheumatoid arthritis synovial fibroblasts

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Sumoylation silences the plasma membrane leak K+ channel K2P1.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

SUMOylation regulates kainate-receptor-mediated synaptic transmission

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

PIASy mediates NEMO sumoylation and NF-kappaB activation in response to genotoxic stress.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A new protease required for cell-cycle progression in yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

SUMO fusion technology for difficult-to-express proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Expression and purification of SARS coronavirus proteins using SUMO-fusions.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Systematic analysis of fusion and affinity tags using human aspartyl-tRNA synthetase expressed in E. coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a coproduct in an ethanologenic Saccharomyces cerevisiae strain

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Enhanced protein expression in mammalian cells using engineered SUMO fusions: secreted phospholipase A2.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Crystal structure of the DsbA protein required for disulphide bond formation in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Identification of a protein required for disulfide bond formation in vivo.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Expression of eukaryotic proteins in soluble form in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Isolation and sequencing of the gene encoding delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Expression as trpE fusion.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and Swine

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Characterization of the family of Mistic homologues

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Mistic: cellular localization, solution behavior, polymerization, and fibril formation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Bacterial expression of a eukaryotic membrane protein in fusion to various Mistic orthologs

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Making the most of fusion tags technology in structural characterization of membrane proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The use of recombinant methods and molecular engineering in protein crystallization

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Application of protein engineering to enhance crystallizability and improve crystal properties

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Cloning vectors for the production of proteins in Dictyostelium discoideum.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Rapid detection of recombinant antibody fragments directed against cell-surface antigens by flow cytometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Crystallization of the first three domains of the human insulin-like growth factor-1 receptor

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The structure of an antigenic determinant in a protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: implications for understanding the molecular etiology of hypogonadotropic hypogonadism

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Different endoplasmic reticulum trafficking and processing pathways for calsequestrin (CSQ) and epitope-tagged CSQ.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Heterodimerization of type II phytochromes in Arabidopsis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A conserved ubiquitin ligase of the nuclear envelope/endoplasmic reticulum that functions in both ER-associated and Matalpha2 repressor degradation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A carboxy-terminal affinity tag for the purification and mass spectrometric characterization of integral membrane proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Binding of retinoids to ABCA4, the photoreceptor ABC transporter associated with Stargardt macular degeneration

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Design, expression, and characterization of a synthetic human cannabinoid receptor and cannabinoid receptor/ G-protein fusion protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Reversible, site-specific immobilization of polyarginine-tagged fusion proteins on mica surfaces

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Polyarginine as a multifunctional fusion tag.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Twenty-five years of immobilized metal ion affinity chromatography: past, present and future

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Automated technologies and novel techniques to accelerate protein crystallography for structural genomics.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Metal chelate affinity chromatography, a new approach to protein fractionation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A novel purification method for histidine-tagged proteins containing a thrombin cleavage site.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

In vitro protein refolding by chromatographic procedures

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Enhanced soluble protein expression using two new fusion tags

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Refolding and purification of histidine-tagged protein by artificial chaperone-assisted metal affinity chromatography.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High-throughput T7 LIC vector for introducing C-terminal poly-histidine tags with variable lengths without extra sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Use of proteomics for design of a tailored host cell for highly efficient protein purification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Assessment of prokaryotic collagen-like sequences derived from streptococcal Scl1 and Scl2 proteins as a source of recombinant GXY polymers

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Comparison of affinity tags for protein purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The use of mRNA display to select high-affinity protein-binding peptides

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A single step purification for recombinant proteins Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP‐dependent protein kinase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Identification of the calmodulin-binding domain of skeletal muscle myosin light chain kinase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Affinity purification of recombinant proteins fused to calmodulin or to calmodulin-binding peptides

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Cloning, nucleotide sequence and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with the corresponding genes from Arthrobacter aurescens DSM 3747.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Intein-mediated protein ligation: harnessing nature's escape artists

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Fusion to a pull-down domain: a novel approach of producing Trigonopsis variabilisD-amino acid oxidase as insoluble enzyme aggregates.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A misfolded but active dimer of bovine seminal ribonuclease.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Thermodynamics of protein-peptide interactions in the ribonuclease S system studied by titration calorimetry

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

An epitope tag derived from human transcription factor IIB that reacts with a polyol-responsive monoclonal antibody.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Advances in gentle immunoaffinity chromatography

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Development of an epitope tag for the gentle purification of proteins by immunoaffinity chromatography: application to epitope-tagged green fluorescent protein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

HaloTag-based purification of functional human kinases from mammalian cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

HaloTag: a novel protein labeling technology for cell imaging and protein analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A generic protein purification method for protein complex characterization and proteome exploration

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A generic method for the production of recombinant proteins in Escherichia coli using a dual hexahistidine-maltose-binding protein affinity tag.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A simple and efficient expression and purification system using two newly constructed vectors

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High-level expression in Saccharomyces cerevisiae enables isolation and spectroscopic characterization of functional human adenosine A2a receptor

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Fusion tails for the recovery and purification of recombinant proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Global analysis of protein expression in yeast

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Functional organization of the yeast proteome by systematic analysis of protein complexes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Decreased secretion and unfolded protein response upregulation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The tandem affinity purification method: an efficient system for protein complex purification and protein interaction identification

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The tandem affinity purification (TAP) method: a general procedure of protein complex purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Recombinant enterokinase light chain with affinity tag: expression from Saccharomyces cerevisiae and its utilities in fusion protein technology

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Expression of catalytically active barley glutamyl tRNAGlu reductase in Escherichia coli as a fusion protein with glutathione S-transferase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Influence of the amino acid residue downstream of (Asp)4Lys on enterokinase cleavage of a fusion protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Preparation of recombinant thioredoxin fused N-terminal proCNP: Analysis of enterokinase cleavage products reveals new enterokinase cleavage sites.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Expression and purification of soluble His(6)-tagged TEV protease

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

A combined approach to improving large-scale production of tobacco etch virus protease

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Ligation-independent cloning of glutathione S-transferase fusion genes for expression in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Determination of oxalic acid in urine by atomic absorption spectrophotometry

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

The role of plasmin in kinin-release by preparations of human thrombin

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High quality protein microarray using in situ protein purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

High-throughput protein purification using an automated set-up for high-yield affinity chromatography.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Crystallization of glutathione S-transferase from human placenta

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201100155

21 January 2018

Identifiers

DOI

10.1002/BIOT.201100155

1 reference

21 June 2017

1 reference

PubMed ID

22442034