Design of high-throughput methods of protein production for structural biology (Q34032380)

30 July 2017

1 reference

title

Design of high-throughput methods of protein production for structural biology (English)

1 reference

30 July 2017

0 references

Stevens RC

1

1 reference

30 July 2017

1 September 2000

1 reference

30 July 2017

1 reference

30 July 2017

8

1 reference

30 July 2017

9

1 reference

30 July 2017

R177-85

1 reference

Cell-free production and stable-isotope labeling of milligram quantities of proteins

30 July 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Protein expression strategies for identification of novel target proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

A generic protein purification method for protein complex characterization and proteome exploration

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Use of T7 RNA polymerase to direct expression of cloned genes

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Ribonuclease S-peptide as a carrier in fusion proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Fusion tails for the recovery and purification of recombinant proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Gene fusion expression systems in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Strategies for optimizing heterologous protein expression in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Enzymatic and chemical cleavage of fusion proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Peptide tags for a dual affinity fusion system.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins--evaluation of two anti-His-tag monoclonal antibodies.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Tandem affinity tags for the purification of bivalent anti-DNA single-chain Fv expressed in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Introduction to expression by fusion protein vectors

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Construction and characterization of a set of E. coli strains deficient in all known loci affecting the proteolytic stability of secreted recombinant proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Crystal structure of tyrosine hydroxylase with bound cofactor analogue and iron at 2.3 A resolution: self-hydroxylation of Phe300 and the pterin-binding site

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Overcoming inclusion body formation in a high-level expression system.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Metal-affinity separations: a new dimension in protein processing

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Expression of correctly folded proteins in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Expression in Escherichia coli: purification and properties of the yeast general transcription factor TFIID.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

A novel strategy for production of a highly expressed recombinant protein in an active form

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

GroE heat-shock proteins promote assembly of foreign prokaryotic ribulose bisphosphate carboxylase oligomers in Escherichia coli.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

In vitro folding of inclusion body proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing Heat-shock proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Escherichia coli skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Solubility of proteins isolated from inclusion bodies is enhanced by fusion to maltose-binding protein or thioredoxin

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Structure and morphology of protein inclusion bodies in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Correctly Folded T-cell Receptor Fragments in the Periplasm of Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Protein folding in the periplasm of Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Building bridges: disulphide bond formation in the cell

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Rapid protein-folding assay using green fluorescent protein

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Solubilization of protein aggregates

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Active papain renatured and processed from insoluble recombinant propapain expressed in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Renaturation of citrate synthase: influence of denaturant and folding assistants

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Correct disulfide pairing and efficient refolding of detergent-solubilized single-chain Fv proteins from bacterial inclusion bodies

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Purification of overproduced Escherichia coli RNA polymerase sigma factors by solubilizing inclusion bodies and refolding from Sarkosyl

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Refolding of recombinant proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Advances in refolding of proteins produced in E. coli

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Refolding, purification, and characterization of a loop deletion mutant of human Bcl-2 from bacterial inclusion bodies.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

A new protein folding screen: application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Microdrop screening: a rapid method to optimize solvent conditions for NMR spectroscopy of proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Use of dynamic light scattering to assess crystallizability of macromolecules and macromolecular assemblies

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Mass spectrometry. From genomics to proteomics

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

[10] Dynamic light scattering in evaluating crystallizability of macromolecules.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Crystallizing proteins - a rational approach?

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

A human cDNA library for high-throughput protein expression screening

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Higher throughput bioanalysis by automation of a protein precipitation assay using a 96-well format with detection by LC-MS/MS

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

An ultrahigh-throughput screening assay for estrogen receptor ligands

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Protein fusions: bioseparation and application

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Cellulose-binding domains: potential for purification of complex proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

Green fluorescent protein as a marker for gene expression

1 reference

https://api.crossref.org/works/10.1016%2FS0969-2126%2800%2900193-3

7 January 2021

10.1016/S0969-2126(00)00193-3

Identifiers

DOI

1 reference

30 July 2017

1 reference

PubMed ID

10986469