SUMO fusion technology for difficult-to-express proteins (Q36220537)

12 August 2017

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SUMO fusion technology for difficult-to-express proteins (English)

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12 August 2017

Tauseef R Butt

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12 August 2017

Suzanne C Edavettal

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2

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12 August 2017

John P Hall

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12 August 2017

Michael R Mattern

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12 August 2017

9 April 2005

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Protein Expression and Purification

12 August 2017

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12 August 2017

43

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12 August 2017

1-9

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12 August 2017

1

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The N-end rule: functions, mysteries, uses

12 August 2017

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12 December 2020

Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein

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12 December 2020

Enhancement of the solubility of proteins overexpressed in Escherichia coli by heat shock

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12 December 2020

Differential gene expression mediated by late, very late and hybrid baculovirus promoters

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12 December 2020

Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli

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12 December 2020

Expression and purification of SARS coronavirus proteins using SUMO-fusions.

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12 December 2020

Printing proteins as microarrays for high-throughput function determination

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12 December 2020

A perspective on protein microarrays

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12 December 2020

Small ubiquitin-like modifier conjugation regulates nuclear export of TEL, a putative tumor suppressor

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12 December 2020

Multiple joined genes prevent product degradation in Escherichia coli

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12 December 2020

SP-RING for SUMO: new functions bloom for a ubiquitin-like protein

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12 December 2020

Strategies for achieving high-level expression of genes in Escherichia coli

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12 December 2020

A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa.

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12 December 2020

Improved secretory production of recombinant proteins by random mutagenesis of hlyB, an alpha-hemolysin transporter from Escherichia coli

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12 December 2020

In vivo half-life of a protein is a function of its amino-terminal residue

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12 December 2020

How important is the molten globule for correct protein folding?

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12 December 2020

Protein modification by SUMO

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12 December 2020

The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein

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12 December 2020

Metal affinity chromatography of recombinant HIV-1 reverse transcriptase containing a human renin cleavable metal binding domain

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12 December 2020

A second proteinase encoded by a plant potyvirus genome

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12 December 2020

A highly specific system for efficient enzymatic removal of tags from recombinant proteins

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12 December 2020

SENP1 enhances androgen receptor-dependent transcription through desumoylation of histone deacetylase 1

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12 December 2020

A nuclear protease required for flowering-time regulation in Arabidopsis reduces the abundance of SMALL UBIQUITIN-RELATED MODIFIER conjugates.

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12 December 2020

SUMO modification of Huntingtin and Huntington's disease pathology

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12 December 2020

Histone sumoylation is associated with transcriptional repression

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12 December 2020

Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins

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12 December 2020

Ubiquitin-like proteins: new wines in new bottles

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12 December 2020

Sumoylation of Pdx1 is associated with its nuclear localization and insulin gene activation

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12 December 2020

Augmentation of protein production by a combination of the T7 RNA polymerase system and ubiquitin fusion: overproduction of the human DNA repair protein, ERCC1, as a ubiquitin fusion protein in Escherichia coli.

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12 December 2020

Recombinant protein expression in Escherichia coli

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12 December 2020

Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus

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12 December 2020

A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex

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12 December 2020

Structure determination of the small ubiquitin-related modifier SUMO-1

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12 December 2020

Upstream strategies to minimize proteolytic degradation upon recombinant production in Escherichia coli

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12 December 2020

Fine architecture of bacterial inclusion bodies

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12 December 2020

Expression of correctly folded proteins in Escherichia coli

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12 December 2020

Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.

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12 December 2020

On the road to repair: PCNA encounters SUMO and ubiquitin modifications

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12 December 2020

An efficient system for high-level expression and easy purification of authentic recombinant proteins

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12 December 2020

Maltose transport system of Escherichia coli: an ABC-type transporter

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12 December 2020

ZNF76, a novel transcriptional repressor targeting TATA-binding protein, is modulated by sumoylation

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12 December 2020

Improved synthesis of Salmonella typhimurium enterotoxin using gene fusion expression systems.

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12 December 2020

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

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12 December 2020

Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme

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12 December 2020

Expression and characterization of a recombinant human parathyroid hormone secreted by Escherichia coli employing the staphylococcal protein A promoter and signal sequence.

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12 December 2020

Expression, purification of human vasostatin120-180 in Escherichia coli, and its anti-angiogenic characterization

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12 December 2020

Sequential modification of NEMO/IKKgamma by SUMO-1 and ubiquitin mediates NF-kappaB activation by genotoxic stress

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12 December 2020

High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product

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12 December 2020

The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer

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12 December 2020

Use of T7 RNA polymerase to direct expression of cloned genes

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12 December 2020

Global shifts in protein sumoylation in response to electrophile and oxidative stress.

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12 December 2020

Evolution and function of ubiquitin-like protein-conjugation systems

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12 December 2020

Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy.

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12 December 2020

The role of the thioredoxin and glutaredoxin pathways in reducing protein disulfide bonds in the Escherichia coli cytoplasm.

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12 December 2020

Co-overexpression of folding modulators improves the solubility of the recombinant guinea pig liver transglutaminase expressed in Escherichia coli

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12 December 2020

Ubiquitin function studied by disulfide engineering.

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12 December 2020

Cellular location affects protein stability in Escherichia coli

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12 December 2020

A new protease required for cell-cycle progression in yeast

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12 December 2020

Fusion tails for the recovery and purification of recombinant proteins

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12 December 2020

Effects of protease inhibitors on protein breakdown in Escherichia coli

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12 December 2020

Intracellular protein degradation in mammalian and bacterial cells

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12 December 2020

SUMO--nonclassical ubiquitin

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12 December 2020

Protein folding intermediates and pathways studied by hydrogen exchange

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12 December 2020

Ubiquitin and its kin: how close are the family ties?

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12 December 2020

SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.

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12 December 2020

High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system.

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12 December 2020

Fusions to maltose-binding protein: control of folding and solubility in protein purification

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12 December 2020

Evidence for a three-state model of protein folding from kinetic analysis of ubiquitin variants with altered core residues

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12 December 2020

The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity

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12 December 2020

Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript.

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12 December 2020

The solubility and stability of recombinant proteins are increased by their fusion to NusA.

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12 December 2020

Global analysis of protein activities using proteome chips

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12 December 2020

Correctly Folded T-cell Receptor Fragments in the Periplasm of Escherichia coli

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12 December 2020

Codon optimization of gene fragments encoding Plasmodium falciparum merzoite proteins enhances DNA vaccine protein expression and immunogenicity in mice.

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12 December 2020

Understanding the art of producing protein and nonprotein molecules in Escherichia coli

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12 December 2020

Desumoylation activity of Axam, a novel Axin-binding protein, is involved in downregulation of beta-catenin

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12 December 2020

SUMO, ubiquitin's mysterious cousin

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12 December 2020

Analysis and control of proteolysis of recombinant proteins in Escherichia coli.

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12 December 2020

The small ubiquitin-like modifier (SUMO) protein modification system in Arabidopsis. Accumulation of SUMO1 and -2 conjugates is increased by stress.

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12 December 2020

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

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12 December 2020

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor

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12 December 2020

10.1016/J.PEP.2005.03.016

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DOI

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12 August 2017

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