High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis (Q56888745)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29931269%20AND%20SRC:MED&resulttype=core&format=json

1 April 2020

title

High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis (English)

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1 April 2020

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5

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1 April 2020

author name string

Derek M Mason

1

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1 April 2020

Cédric R Weber

2

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1 April 2020

Cristina Parola

3

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1 April 2020

Simon M Meng

4

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1 April 2020

William J Kelton

6

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1 April 2020

Sai T Reddy

7

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1 April 2020

language of work or name

English

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publication date

1 August 2018

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1 April 2020

published in

Nucleic Acids Research

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1 April 2020

volume

46

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1 April 2020

page(s)

7436-7449

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1 April 2020

issue

14

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Creative Commons Attribution-NonCommercial 4.0 International

1 April 2020

copyright license

start time

21 June 2018

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April 2022 Public Data File from Crossref

copyright status

copyrighted

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describes a project that uses

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10 June 2022

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pmc&rettype=xml&id=6101513

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2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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Coupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodies

2 November 2018

1 reference

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2 November 2018

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High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

2 November 2018

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Deep mutational scanning: assessing protein function on a massive scale

2 November 2018

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High-resolution mapping of protein sequence-function relationships

2 November 2018

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2 November 2018

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2 November 2018

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2 November 2018

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Automated design of degenerate codon libraries

2 November 2018

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Selecting and screening recombinant antibody libraries

2 November 2018

1 reference

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Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity.

2 November 2018

1 reference

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High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold

2 November 2018

1 reference

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Estimating the diversity of peptide populations from limited sequence data

2 November 2018

1 reference

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Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library

2 November 2018

1 reference

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Diversity in the CDR3 region of V(H) is sufficient for most antibody specificities

2 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6101513

Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides

2 November 2018

1 reference

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Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.

2 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6101513

Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis

2 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6101513

Phage antibodies: filamentous phage displaying antibody variable domains

2 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6101513

Continuous cultures of fused cells secreting antibody of predefined specificity

2 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=6101513

2 November 2018

Identifiers

DOI

10.1093/NAR/GKY550

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29931269%20AND%20SRC:MED&resulttype=core&format=json

1 April 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:29931269%20AND%20SRC:MED&resulttype=core&format=json

1 April 2020

1 reference