Tinkering with osmotically controlled transcription allows enhanced production and excretion of ectoine and hydroxyectoine from a microbial cell factory. (Q47661134)

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English	Tinkering with osmotically controlled transcription allows enhanced production and excretion of ectoine and hydroxyectoine from a microbial cell factory.	scientific article

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8 April 2020

Tinkering with Osmotically Controlled Transcription Allows Enhanced Production and Excretion of Ectoine and Hydroxyectoine from a Microbial Cell Factory (English)

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8 April 2020

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8 April 2020

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Sebastian Poehl

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8 April 2020

Philipp Hub

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8 April 2020

Erhard Bremer

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Nadine Stöveken

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2 January 2018

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8 April 2020

Applied and Environmental Microbiology

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8 April 2020

Ectoines in cell stress protection: uses and biotechnological production.

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21 January 2018

Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments

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21 January 2018

Biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms

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21 January 2018

Strangers in the archaeal world: osmostress-responsive biosynthesis of ectoine and hydroxyectoine by the marine thaumarchaeon Nitrosopumilus maritimus.

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21 January 2018

Osmoadaptative Strategy and Its Molecular Signature in Obligately Halophilic Heterotrophic Protists

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21 January 2018

A molecular mechanism for osmolyte-induced protein stability

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Osmolyte-induced conformational changes in the Hsp90 molecular chaperone

1 reference

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21 January 2018

Origins of the osmoprotective properties of betaine and proline in Escherichia coli K-12

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21 January 2018

Osmotic Shock Induced Protein Destabilization in Living Cells and Its Reversal by Glycine Betaine

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21 January 2018

Bacterial osmoregulation: a paradigm for the study of cellular homeostasis

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Osmosensing and osmoregulatory compatible solute accumulation by bacteria

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21 January 2018

Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.

1 reference

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21 January 2018

A specialized aspartokinase enhances the biosynthesis of the osmoprotectants ectoine and hydroxyectoine in Pseudomonas stutzeri A1501

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https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Heterologous ectoine production in Escherichia coli: by-passing the metabolic bottle-neck.

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21 January 2018

Characterization of the ectoine biosynthesis genes of haloalkalotolerant obligate methanotroph "Methylomicrobium alcaliphilum 20Z".

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21 January 2018

Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase.

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Crystal structure of the ectoine hydroxylase, a snapshot of the active site

1 reference

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21 January 2018

Characterization of genes for the biosynthesis of the compatible solute ectoine from Marinococcus halophilus and osmoregulated expression in Escherichia coli

1 reference

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21 January 2018

Osmotically regulated synthesis of the compatible solute ectoine in Bacillus pasteurii and related Bacillus spp.

1 reference

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21 January 2018

Unravelling the adaptation responses to osmotic and temperature stress in Chromohalobacter salexigens, a bacterium with broad salinity tolerance

1 reference

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21 January 2018

A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T.

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21 January 2018

Complex regulation of the synthesis of the compatible solute ectoine in the halophilic bacterium Chromohalobacter salexigens DSM 3043T.

1 reference

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21 January 2018

Identification and characterization of EctR1, a new transcriptional regulator of the ectoine biosynthesis genes in the halotolerant methanotroph Methylomicrobium alcaliphilum 20Z.

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

The transcriptional regulator, CosR, controls compatible solute biosynthesis and transport, motility and biofilm formation in Vibrio cholerae

1 reference

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21 January 2018

GlnR-Mediated Regulation of ectABCD Transcription Expands the Role of the GlnR Regulon to Osmotic Stress Management

1 reference

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21 January 2018

Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses

1 reference

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21 January 2018

Ectoine and hydroxyectoine as protectants against osmotic and cold stress: uptake through the SigB-controlled betaine-choline- carnitine transporter-type carrier EctT from Virgibacillus pantothenticus

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

The ectD gene, which is involved in the synthesis of the compatible solute hydroxyectoine, is essential for thermoprotection of the halophilic bacterium Chromohalobacter salexigens.

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy in Vibrio anguillarum against cold stress.

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21 January 2018

Neutrons describe ectoine effects on water H-bonding and hydration around a soluble protein and a cell membrane

1 reference

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21 January 2018

Influence of the Compatible Solute Ectoine on the Local Water Structure: Implications for the Binding of the Protein G5P to DNA.

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21 January 2018

Extrinsic protein stabilization by the naturally occurring osmolytes beta-hydroxyectoine and betaine

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21 January 2018

Glass-forming property of hydroxyectoine is the cause of its superior function as a desiccation protectant

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21 January 2018

Characterization of the synthetic compatible solute homoectoine as a potent PCR enhancer.

1 reference

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21 January 2018

Hydroxyectoine is superior to trehalose for anhydrobiotic engineering of Pseudomonas putida KT2440.

1 reference

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21 January 2018

High survival and stability rates of Escherichia coli dried in hydroxyectoine

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https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

The effect of compatible solute ectoines on the structural organization of lipid monolayer and bilayer membranes

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Membrane protein stability depends on the concentration of compatible solutes--a single molecule force spectroscopic study

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Compatible-solute-supported periplasmic expression of functional recombinant proteins under stress conditions

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Extremolytes: Natural compounds from extremophiles for versatile applications

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

The multifunctional role of ectoine as a natural cell protectant

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Bacterial mechanosensitive channels: progress towards an understanding of their roles in cell physiology

1 reference

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21 January 2018

Feeding on compatible solutes: A substrate-induced pathway for uptake and catabolism of ectoines and its genetic control by EnuR.

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

New type of osmoregulated solute transporter identified in halophilic members of the bacteria domain: TRAP transporter TeaABC mediates uptake of ectoine and hydroxyectoine in Halomonas elongata DSM 2581(T).

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Process optimization of the integrated synthesis and secretion of ectoine and hydroxyectoine under hyper/hypo-osmotic stress

1 reference

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21 January 2018

High-yield cultivation of Marinococcus M52 for production and recovery of hydroxyectoine.

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Optimization of ectoine synthesis through fed-batch fermentation of Brevibacterium epidermis

1 reference

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21 January 2018

Efficient production of ectoine using ectoine-excreting strain

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21 January 2018

Natural and engineered hydroxyectoine production based on the Pseudomonas stutzeri ectABCD-ask gene cluster

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Continuous synthesis and excretion of the compatible solute ectoine by a transgenic, nonhalophilic bacterium.

1 reference

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21 January 2018

Improved fermentative production of the compatible solute ectoine by Corynebacterium glutamicum from glucose and alternative carbon sources.

1 reference

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21 January 2018

Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine

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21 January 2018

Synthesis and release of the bacterial compatible solute 5-hydroxyectoine in Hansenula polymorpha

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21 January 2018

Pathway construction and metabolic engineering for fermentative production of ectoine in Escherichia coli.

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens

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21 January 2018

Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501.

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21 January 2018

Osmoprotection of Escherichia coli by ectoine: uptake and accumulation characteristics

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21 January 2018

Osmotic control of opuA expression in Bacillus subtilis and its modulation in response to intracellular glycine betaine and proline pools

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21 January 2018

Osmotically controlled synthesis of the compatible solute proline is critical for cellular defense of Bacillus subtilis against high osmolarity

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21 January 2018

Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity

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21 January 2018

Profiling early osmostress-dependent gene expression in Escherichia coli using DNA macroarrays

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21 January 2018

From acids to osmZ: multiple factors influence synthesis of the OmpF and OmpC porins in Escherichia coli.

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21 January 2018

Cyclic AMP-dependent osmoregulation of crp gene expression in Escherichia coli

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21 January 2018

Random mutagenesis of global transcription factor cAMP receptor protein for improved osmotolerance

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21 January 2018

The cAMP receptor protein CRP can function as an osmoregulator of transcription in Escherichia coli

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21 January 2018

Bacterial sigma factors: a historical, structural, and genomic perspective

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21 January 2018

The -16 region of Bacillus subtilis and other gram-positive bacterial promoters

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21 January 2018

Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli

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21 January 2018

Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli

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21 January 2018

EctD-mediated biotransformation of the chemical chaperone ectoine into hydroxyectoine and its mechanosensitive channel-independent excretion

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21 January 2018

High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli

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21 January 2018

The ion coupling and organic substrate specificities of osmoregulatory transporter ProP in Escherichia coli.

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21 January 2018

High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection

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21 January 2018

Remodeling and activation of Escherichia coli RNA polymerase by osmolytes

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21 January 2018

Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaine

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21 January 2018

A physiological role for DNA supercoiling in the osmotic regulation of gene expression in S. typhimurium and E. coli

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21 January 2018

Local DNA topology and gene expression: the case of the leu-500 promoter

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21 January 2018

All three elements of the lac ps promoter mediate its transcriptional response to DNA supercoiling

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21 January 2018

Transient and dynamic DNA supercoiling potently stimulates the leu-500 promoter in Escherichia coli

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21 January 2018

DNA supercoiling - a global transcriptional regulator for enterobacterial growth?

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21 January 2018

Characterization of three novel mechanosensitive channel activities in Escherichia coli.

1 reference

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21 January 2018

Molecular analysis of the ggtBCD gene cluster of Synechocystis sp. strain PCC6803 encoding subunits of an ABC transporter for osmoprotective compounds

1 reference

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21 January 2018

Synthesis, release, and recapture of compatible solute proline by osmotically stressed Bacillus subtilis cells

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation.

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21 January 2018

Small multidrug resistance protein EmrE reduces host pH and osmotic tolerance to metabolic quaternary cation osmoprotectants

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21 January 2018

Efflux systems in bacteria and their metabolic engineering applications

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21 January 2018

Protection of Bacillus subtilis against cold stress via compatible-solute acquisition

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21 January 2018

Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12

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21 January 2018

Identification and molecular analysis of glgS, a novel growth-phase-regulated and rpoS-dependent gene involved in glycogen synthesis in Escherichia coli

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21 January 2018

Identification of a central regulator of stationary-phase gene expression inEscherichia coli

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21 January 2018

Synthesis of the Escherichia coli K-12 nucleoid-associated DNA-binding protein H-NS is subjected to growth-phase control and autoregulation.

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https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes

1 reference

https://api.crossref.org/works/10.1128%2FAEM.01772-17

21 January 2018

Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis

1 reference

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2 November 2018

Bacterial milking: A novel bioprocess for production of compatible solutes

1 reference

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2 November 2018

Characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, Halomonas elongata.

1 reference

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2 November 2018

Interactions of the nucleoid-associated DNA-binding protein H-NS with the regulatory region of the osmotically controlled proU operon of Escherichia coli.

1 reference

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2 November 2018

Adaptation of Escherichia coli to high osmolarity environments: osmoregulation of the high-affinity glycine betaine transport system proU.

1 reference

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2 November 2018

Promoter mutation causing catabolite repression of the Salmonella typhimurium leucine operon

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2 November 2018

Trehalase of Escherichia coli. Mapping and cloning of its structural gene and identification of the enzyme as a periplasmic protein induced under high osmolarity growth conditions

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2 November 2018

Physiological and genetic responses of bacteria to osmotic stress

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2 November 2018

Efflux of choline and glycine betaine from osmoregulating cells of Escherichia coli

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2 November 2018

Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu

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2 November 2018

Compatible solutes as protectants for zymogens against proteolysis

1 reference

https://pubmed.ncbi.nlm.nih.gov/29101191

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression

1 reference

https://pubmed.ncbi.nlm.nih.gov/29101191

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Glycine betaine-assisted protein folding in a lysA mutant of Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/29101191

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Characterization of the osmoregulated Escherichia coli proU promoter and identification of ProV as a membrane-associated protein

1 reference

https://pubmed.ncbi.nlm.nih.gov/29101191

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Ectoine-mediated protection of enzyme from the effect of pH and temperature stress: a study using Bacillus halodurans xylanase as a model

1 reference

https://pubmed.ncbi.nlm.nih.gov/29101191

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The osmoprotectant proline betaine is a major substrate for the binding-protein-dependent transport system ProU of Escherichia coli K-12

1 reference

https://pubmed.ncbi.nlm.nih.gov/29101191

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Natural osmolytes remodel the aggregation pathway of mutant huntingtin exon 1

1 reference

https://pubmed.ncbi.nlm.nih.gov/29101191

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1128/AEM.01772-17

1 reference

Europe PubMed Central

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8 April 2020

1 reference

Europe PubMed Central

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8 April 2020

1 reference

Europe PubMed Central

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8 April 2020

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