Detection of enzymatic activity of transfer RNA modification enzymes using radiolabeled tRNA substrates (Q36900497)

17 August 2017

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title

Detection of enzymatic activity of transfer RNA modification enzymes using radiolabeled tRNA substrates (English)

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17 August 2017

1

Henri Grosjean

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17 August 2017

Louis Droogmans

2

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17 August 2017

Martine Roovers

series ordinal

3

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17 August 2017

Gérard Keith

series ordinal

4

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17 August 2017

language of work or name

English

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publication date

1 January 2007

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17 August 2017

Methods in Enzymology

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17 August 2017

425

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17 August 2017

55-101

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Codon recognition patterns as deduced from sequences of the complete set of transfer RNA species in Mycoplasma capricolum. Resemblance to mitochondria

17 August 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Comparative genomics and evolution of proteins involved in RNA metabolism

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Mechanism, specificity and general properties of the yeast enzyme catalysing the formation of inosine 34 in the anticodon of transfer RNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Role of post-transcriptional modifications of primer tRNALys,3 in the fidelity and efficacy of plus strand DNA transfer during HIV-1 reverse transcription

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Four newly located pseudouridylate residues in Escherichia coli 23S ribosomal RNA are all at the peptidyltransferase center: analysis by the application of a new sequencing technique.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Pseudouridine and ribothymidine formation in the tRNA-like domain of turnip yellow mosaic virus RNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Preparation of a pG-fragment from tRNA(Phe)(yeast) by chemical scission at the dihydrouracil, and inhibition of tRNA(Phe)(yeast) charging by this fragment when combined with the -CCA half of this tRNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Primer extension analysis of RNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic replacement of the anticodon of yeast phenylalanine transfer ribonucleic acid

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

A pseudoknotted tRNA variant is a substrate for tRNA (cytosine-5)-methyltransferase from Xenopus laevis.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Sequence-structure-function studies of tRNA:m5C methyltransferase Trm4p and its relationship to DNA:m5C and RNA:m5U methyltransferases

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic replacement in vitro of the first anticodon base of yeast tRNAAsp: application to the study of tRNA maturation in vivo, after microinjection into frog oocytes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

The yeast Saccharomyces cerevisiae YDL112w ORF encodes the putative 2'-O-ribose methyltransferase catalyzing the formation of Gm18 in tRNAs

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Detection and determination of 5,6-dihydrouridine and 4-thiouridine in transfer ribonucleic acid from different sources

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Biosynthesis of pseudouridine in the in vitro transcribed tRNATyr precursor

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Transfer RNA modification enzymes from Pyrococcus furiosus: detection of the enzymatic activities in vitro

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Characterisation and enzymatic properties of tRNA(guanine 26, N (2), N (2))-dimethyltransferase (Trm1p) from Pyrococcus furiosus

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic 2'-O-methylation of the wobble nucleoside of eukaryotic tRNAPhe: specificity depends on structural elements outside the anticodon loop

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic conversion of guanosine 3' adjacent to the anticodon of yeast tRNAPhe to N1-methylguanosine and the wye nucleoside: dependence on the anticodon sequence.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

2′-O-methylation and inosine formation in the wobble position of anticodon-substituted tRNA-Phe in a homologous yeast in vitro system

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

MODOMICS: a database of RNA modification pathways

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

DNA cytosine methylation and heat-induced deamination

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Post-transcriptlonal modification of the wobble nucletide in anticodon-substituted yeast tRNAIIArgafter microinjection intoxenopus laevis oocytes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

The in vivo stability, maturation and aminoacylation of anticodon-substituted Escherichia coli initiator methionine tRNAs

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Guanosine modifications in runoff transcripts of synthetic transfer RNA-Phe genes microinjected into Xenopus oocytes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Posttranscriptionally modified nucleosides in transfer RNA: their locations and frequencies

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic formation of modified nucleosides in tRNA: dependence on tRNA architecture.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Rapid print-readout technique for sequencing of RNA's containing modified nucleotides

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

An improved separation procedure for nucleoside monophosphates on polyethyleneimine-(PEI-)cellulose thin layers

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

2'-O-Methylated oligonucleotides in ribosomal 18S and 28S RNA of a mouse hepatoma, MH 134

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic conversion of adenosine to inosine in the wobble position of yeast tRNAAsp: the dependence on the anticodon sequence

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic formation of queuosine and of glycosyl queuosine in yeast tRNAs microinjected into Xenopus laevis oocytes. The effect of the anticodon loop sequence

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Post-transcriptional nucleotide modification and alternative folding of RNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Nuclear control of cloverleaf structure of human mitochondrial tRNA(Lys).

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Ribonuclease preparation for the base analysis of polyribonucleotides

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

tRNA transfers to the limelight

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Hydrolysis of dihydrouridine and related compounds

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Purification of RNA molecules by gel techniques

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Mobilities of modified ribonucleotides on two-dimensional cellulose thin-layer chromatography

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Analysis of modified nucleosides and nucleotide sequence of tRNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

The 18S rRNA dimethylase Dim1p is required for pre-ribosomal RNA processing in yeast.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Specific hybridization of tyrosine transfer ribonucleic acids with DNA from a transducing bacteriophage phi-80 carrying the amber suppressor gene su 3

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Reverse transcriptase sequencing of ribosomal RNA for phylogenetic analysis

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Thiolated nucleotides in yeast transfer RNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Summary: the modified nucleosides of RNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Rate of depurination of native deoxyribonucleic acid

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Heat-induced deamination of cytosine residues in deoxyribonucleic acid

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

RNA polymerase bypass at sites of dihydrouracil: implications for transcriptional mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Pseudouridylation (Psi) of U2 snRNA in S. cerevisiae is catalyzed by an RNA-independent mechanism

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Classical and novel approaches to the detection and localization of the numerous modified nucleotides in eukaryotic ribosomal RNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

tRNomics: analysis of tRNA genes from 50 genomes of Eukarya, Archaea, and Bacteria reveals anticodon-sparing strategies and domain-specific features

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

The preparation and some properties of a low molecular weight ribonucleic acid from baker's yeast.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Detection of inosine in messenger RNA by inosine-specific cleavage.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Mapping pseudouridines in RNA molecules

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Inosine exists in mRNA at tissue-specific levels and is most abundant in brain mRNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Direct chemical method for sequencing RNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Splitting of phenylalanine specific tRNA into half molecules by chemical means

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

MRM2 encodes a novel yeast mitochondrial 21S rRNA methyltransferase

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Formation of the conserved pseudouridine at position 55 in archaeal tRNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

The RNA Modification Database: 1999 update

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Determination of Nearest Neighbors in Nucleic Acids by Mass Spectrometry

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Selective modification of nucleosides of tRNA precursors accumulated in a temperature sensitive mutant of Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Nucleotide Modification In Vitro of the Precursor of Transfer RNA Tyr of Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

3 Use of in Vitro32P labeling in the sequence analysis of nonradioactive tRNAs

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

A different approach to RNA sequencing

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Taurine as a constituent of mitochondrial tRNAs: new insights into the functions of taurine and human mitochondrial diseases

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acids

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Intron-dependent formation of pseudouridines in the anticodon of Saccharomyces cerevisiae minor tRNA(Ile).

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Purifying RNA by column chromatography

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Stabilization of nucleic acids by unusual polyamines produced by an extreme thermophile, Thermus thermophilus

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Batchwise purification of specific tRNAs by a solid-phase DNA probe

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Identity elements required for enzymatic formation of N2,N2-dimethylguanosine from N2-monomethylated derivative and its possible role in avoiding alternative conformations in archaeal tRNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Reactions of 2-thioribothymidine ad 4-thiouridine with hydrogen peroxide in transfer ribonucleic acids from Thermus thermophilus and Escherichia coli as studied by circular dichroism

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Enzymatic methylations: III. Cadaverine-induced conformational changes of E.coli tRNAfMetas evidenced by the availability of a specific adenosine and a specific cytidine residue for methylation

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE): quantitative RNA structure analysis at single nucleotide resolution

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

A specific chemical chain scission of tRNA at 7-methylguanosine

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

53 Replacement of odd bases in tRNA by fluorescent dyes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Tertiary structure interactions of 7-methylguanosine in yeast tRNA Phe as studied by borohydride reduction

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Reverse transcription of tRNA.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Modified nucleotides in Bacillus subtilis tRNA(Trp) hyperexpressed in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Reverse transcriptase pauses at N2-methylguanine during in vitro transcription of Escherichia coli 16S ribosomal RNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

A sequence from Drosophila melanogaster 18S rRNA bearing the conserved hypermodified nucleoside am psi: analysis by reverse transcription and high-performance liquid chromatography

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Aminoacylation and micleoside modification of in vitro synthesised transfer RNA

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Detection and quantitation of RNA base modifications

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

RNA design by in vitro RNA recombination and synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

Isolation and characterization of antisuppressor mutations in Escherichia coli

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2807%2925003-7

7 January 2021

10.1016/S0076-6879(07)25003-7

Identifiers

DOI

1 reference

17 August 2017

1 reference