Protein expression systems for structural genomics and proteomics (Q35053327)

6 August 2017

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title

Protein expression systems for structural genomics and proteomics (English)

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6 August 2017

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Shigeyuki Yokoyama

1

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6 August 2017

1 February 2003

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Current Opinion in Chemical Biology

6 August 2017

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6 August 2017

7

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6 August 2017

39-43

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6 August 2017

1

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Structural genomics: opportunities and challenges

6 August 2017

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Completeness in structural genomics.

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Structural genomics of microbes: an objective

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Structural proteomics of an archaeon

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

1 reference

7 January 2021

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

An NMR approach to structural proteomics

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Structural genomics and signaling domains.

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Structural proteomics: prospects for high throughput sample preparation

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Protein production: feeding the crystallographers and NMR spectroscopists

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Accelerating code to function: sizing up the protein production line

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Parallel cloning, expression, purification and crystallization of human proteins for structural genomics

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

High throughput methods for gene cloning and expression.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Screening for soluble expression of recombinant proteins in a 96-well format

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

A solubility-enhancement tag (SET) for NMR studies of poorly behaving proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Coupling of folding and binding for unstructured proteins

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Rapid protein-folding assay using green fluorescent protein

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Engineering soluble proteins for structural genomics.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Random PCR-based screening for soluble domains using green fluorescent protein

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

A continuous cell-free translation system capable of producing polypeptides in high yield

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Cell-free production and stable-isotope labeling of milligram quantities of proteins

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis.

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https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Dual amino acid-selective and site-directed stable-isotope labeling of the human c-Ha-Ras protein by cell-free synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Cell-free translation reconstituted with purified components

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

A bilayer cell-free protein synthesis system for high-throughput screening of gene products.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

A cell-free protein synthesis system for high-throughput proteomics

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Cell-free protein synthesis on a microchip.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Expression of Fab fragment of catalytic antibody 6D9 in an Escherichia coli in vitro coupled transcription/translation system

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

An improved protein bioreactor: efficient product isolation during in vitro protein biosynthesis via affinity tag.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

NMR monitoring of accumulation and folding of 15N-labeled protein overexpressed in Pichia pastoris

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach.

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

Selenomethionine incorporation into a protein by cell-free synthesis

1 reference

https://api.crossref.org/works/10.1016%2FS1367-5931%2802%2900019-4

7 January 2021

10.1016/S1367-5931(02)00019-4

Identifiers

DOI

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6 August 2017

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PubMed ID

12547425