Engineering thermal stability of L-asparaginase by in vitro directed evolution (Q33409731)

26 July 2017

Engineering thermal stability of L-asparaginase by in vitro directed evolution (English)

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26 July 2017

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Nikolaos E Labrou

2

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author name string

Georgia A Kotzia

series ordinal

1

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PubMed ID

19220855

26 July 2017

language of work or name

English

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publication date

13 February 2009

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26 July 2017

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26 July 2017

276

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26 July 2017

6

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26 July 2017

1750-1761

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L-asparaginase and PEG asparaginase--past, present, and future

26 July 2017

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Use of ?-asparaginase in childhood ALL

21 January 2018

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https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Enzymes as agents for the treatment of disease

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

In vivo half life of nanoencapsulated L-asparaginase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Asparagine synthetase in normal and malignant tissues; correlation with tumor sensitivity to asparaginase

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Serum asparaginase activities and asparagine concentrations in the cerebrospinal fluid after a single infusion of 2,500 IU/m(2) PEG asparaginase in children with ALL treated according to protocol COALL-06-97.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Erwinia chrysanthemi L-asparaginase: epitope mapping and production of antigenically modified enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Extracellular expression and single step purification of recombinant Escherichia coli L-asparaginase II.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Effects of polyethylene glycol attachment on physicochemical and biological stability of E. coli L-asparaginase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Cloning, expression and characterisation of Erwinia carotovora L-asparaginase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

L-Asparaginase from Erwinia Chrysanthemi 3937: cloning, expression and characterization.

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Suppression of murine leukemias by L-asparaginase. Incidence of sensitivity among leukemias of various types: comparative inhibitory activities of guinea pig serum L-asparaginase and Escherichia coli L-asparaginase

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

PEGylation, successful approach to drug delivery

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Tailoring structure-function properties of L-asparaginase: engineering resistance to trypsin cleavage

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Stability of biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Improvement of oxidative and thermostability of N-carbamyl-d-amino Acid amidohydrolase by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Directed evolution of enzyme stability

21 January 2018

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https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

High-throughput screening for enhanced protein stability

21 January 2018

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https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Calorimetrically determined dynamics of complex unfolding transitions in proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Glutathione transferase P1-1: self-preservation of an anti-cancer enzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Structural basis for the activity and substrate specificity of Erwinia chrysanthemi L-asparaginase

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Free-energy simulations and experiments reveal long-range electrostatic interactions and substrate-assisted specificity in an aminoacyl-tRNA synthetase

21 January 2018

1 reference

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Contributions of long-range electrostatic interactions to 4-chlorobenzoyl-CoA dehalogenase catalysis: a combined theoretical and experimental study

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

An efficient one-step site-directed and site-saturation mutagenesis protocol

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Close-range electrostatic interactions in proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Increasing protein stability by altering long-range coulombic interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Structural and functional stabilization of L-asparaginase via multisubunit immobilization onto highly activated supports

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

Beta-aspartylpeptides as substrates of L-asparaginases from Escherichia coli and Erwinia chrysanthemi

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

WHAT IF: a molecular modeling and drug design program

21 January 2018

1 reference

https://api.crossref.org/works/10.1111%2FJ.1742-4658.2009.06910.X

SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling

21 January 2018

1 reference

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

21 January 2018

1 reference

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10.1111/J.1742-4658.2009.06910.X

21 January 2018

Identifiers

DOI

1 reference

26 July 2017

1 reference

PubMed ID

19220855