Spheroid-based drug screen: considerations and practical approach (Q30052394)

25 May 2017

4

Leoni A Kunz-Schughart

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25 May 2017

author name string

Juergen Friedrich

series ordinal

1

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25 May 2017

Claudia Seidel

series ordinal

2

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25 May 2017

Reinhard Ebner

series ordinal

3

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25 May 2017

publication date

February 2009

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25 May 2017

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25 May 2017

volume

4

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25 May 2017

page(s)

309-324

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25 May 2017

issue

3

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Organotypic keratinocyte cocultures in defined medium with regular epidermal morphogenesis and differentiation

25 May 2017

cites work

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Drug penetration in solid tumours

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Experimental approaches to kinetics of gas diffusion in hydrogenase

27 May 2017

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Identification of human brain tumour initiating cells

27 May 2017

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Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer

27 May 2017

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A human colon cancer cell capable of initiating tumour growth in immunodeficient mice

27 May 2017

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Identification and expansion of human colon-cancer-initiating cells

27 May 2017

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CD133: molecule of the moment

27 May 2017

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Integrative genomic analyses identify MITF as a lineage survival oncogene amplified in malignant melanoma

27 May 2017

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Cell culture: biology's new dimension

27 May 2017

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

The use of 3-D cultures for high-throughput screening: the multicellular spheroid model

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

A reliable tool to determine cell viability in complex 3-d culture: the acid phosphatase assay

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

To create the correct microenvironment: three-dimensional heterotypic collagen assays for human breast epithelial morphogenesis and neoplasia

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Modeling dynamic reciprocity: engineering three-dimensional culture models of breast architecture, function, and neoplastic transformation

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Three-dimensional culture models of normal and malignant breast epithelial cells

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Experimental anti-tumor therapy in 3-D: spheroids--old hat or new challenge?

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Multicellular spheroids. A review on cellular aggregates in cancer research

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Multicellular spheroids: a three-dimensional in vitro culture system to study tumour biology

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Three-dimensional spheroid model in tumor biology

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Establishment and characterization of multicellular spheroids from a human glioma cell line; Implications for tumor therapy

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Spheroids in radiobiology and photodynamic therapy

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Resistance of tumor cells to chemo- and radiotherapy modulated by the three-dimensional architecture of solid tumors and spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Therapeutic implications of cancer stem cells.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Mammalian target of rapamycin contributes to the acquired apoptotic resistance of human mesothelioma multicellular spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Multicellular resistance: a paradigm for clinical resistance?

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Tumor biology and experimental therapeutics

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Relations between pH, oxygen partial pressure and growth in cultured cell spheroids.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Comparison of protein expression profiles between monolayer and spheroid cell culture of HT-29 cells revealed fragmentation of CK18 in three-dimensional cell culture

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Identification of genes differentially expressed in V79 cells grown as multicell spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Antitumor activity of rapamycin in a transgenic mouse model of ErbB2-dependent human breast cancer

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Identification of differentially expressed genes involved in the formation of multicellular tumor spheroids by HT-29 colon carcinoma cells.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

The phosphatidylinositol 3-kinase inhibitor, PX-866, is a potent inhibitor of cancer cell motility and growth in three-dimensional cultures

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Multicellular tumor spheroids: intermediates between monolayer culture and in vivo tumor

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Brave little world: spheroids as an in vitro model to study tumor-immune-cell interactions

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Monocyte chemoattractant protein-1 (MCP-1) inhibits the intestinal-like differentiation of monocytes

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Polyphenols prevent cell shedding from mouse mammary cancer spheroids and inhibit cancer cell invasion in confrontation cultures derived from embryonic stem cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Tumor microenvironment and drug resistance in hematologic malignancies

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Epidermal homeostasis in long-term scaffold-enforced skin equivalents

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Integration of endothelial cells in multicellular spheroids prevents apoptosis and induces differentiation

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Rapid generation of single-tumor spheroids for high-throughput cell function and toxicity analysis

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Effects of irradiation and cisplatin on human glioma spheroids: inhibition of cell proliferation and cell migration

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Screening for supra-additive effects of cytotoxic drugs and gamma irradiation in an in vitro model for hepatocellular carcinoma

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Cell and environment interactions in tumor microregions: the multicell spheroid model

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Microenvironmental regulation of proliferation in multicellular spheroids is mediated through differential expression of cyclin-dependent kinase inhibitors

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Gene expression analysis of tumor spheroids reveals a role for suppressed DNA mismatch repair in multicellular resistance to alkylating agents

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Down-regulation of DNA mismatch repair proteins in human and murine tumor spheroids: implications for multicellular resistance to alkylating agents.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Regulation of DNA repair in hypoxic cancer cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Glutathione content of V79 cells in two- or three-dimensional culture

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Three-dimensional culture regulates Raf-1 expression to modulate fibronectin matrix assembly

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Liquid-overlay culture of cellular spheroids.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Long-term culture of adult rat hepatocyte spheroids.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Reexpression of the TJ protein CLDN1 induces apoptosis in breast tumor spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Development of an intrinsic P-glycoprotein-mediated doxorubicin resistance in quiescent cell layers of large, multicellular prostate tumor spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Adaptation of an automated selective dissociation procedure to two novel spheroid types

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

In vitro chemosensitivity testing using the multicellular tumor spheroid model.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Slow penetration of anthracyclines into spheroids and tumors: a therapeutic advantage?

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Automated selective dissociation of cells from different regions of multicellular spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

The chemopreventive flavonoid apigenin confers radiosensitizing effect in human tumor cells grown as monolayers and spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Three-dimensional cell culture induces novel proliferative and metabolic alterations associated with oncogenic transformation

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

A model of quiescent tumour microregions for evaluating multicellular resistance to chemotherapeutic drugs.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Idarubicin and idarubicinol effects on breast cancer multicellular spheroids.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Assessment of supra-additive effects of cytotoxic drugs and low dose rate irradiation in an in vitro model for hepatocellular carcinoma.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Effects of ALA-mediated photodynamic therapy on the invasiveness of human glioma cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Global gene expression analysis of early response to chemotherapy treatment in ovarian cancer spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Differential expression of CD44(S) and variant isoforms v3, v10 in three-dimensional cultures of mouse melanoma cell lines

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Molecular description of a 3D in vitro model for the study of epithelial ovarian cancer (EOC).

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Nuclear oxygen sensing: induction of endogenous prolyl-hydroxylase 2 activity by hypoxia and nitric oxide

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Lithium inhibits invasion of glioma cells; possible involvement of glycogen synthase kinase-3

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Thymoquinone triggers inactivation of the stress response pathway sensor CHEK1 and contributes to apoptosis in colorectal cancer cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Reactive oxygen species-linked regulation of the multidrug resistance transporter P-glycoprotein in Nox-1 overexpressing prostate tumor spheroids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Glioblastoma-derived tumorospheres identify a population of tumor stem-like cells with angiogenic potential and enhanced multidrug resistance phenotype.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Gastric cancer stem cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Karyotypic "state" as a potential determinant for anticancer drug discovery

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Further evidence to support the melanocytic origin of MDA-MB-435

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.226

Electrical fields enhance growth of cancer spheroids by reactive oxygen species and intracellular Ca2+

31 March 2020

1 reference

12 December 2020

A radioresistant variant derived from a human neuroblastoma cell line is less prone to radiation-induced apoptosis

1 reference

12 December 2020

Response of LNCaP spheroids after treatment with an alpha-particle emitter (213Bi)-labeled anti-prostate-specific membrane antigen antibody (J591).

1 reference

12 December 2020

Three-dimensional cell cultures: from molecular mechanisms to clinical applications

1 reference

12 December 2020

Combination treatment with 17-N-allylamino-17-demethoxy geldanamycin and acute irradiation produces supra-additive growth suppression in human prostate carcinoma spheroids.

1 reference

12 December 2020

Distribution of photosensitizers in bladder cancer spheroids: implications for intravesical instillation of photosensitizers for photodynamic therapy of bladder cancer.

1 reference

12 December 2020

Tumor growth in vivo and as multicellular spheroids compared by mathematical models

1 reference

12 December 2020

Comparison of various methods of assaying the cytotoxic effects of ethanol on human hepatoblastoma cells (HUH-6 line).

1 reference

12 December 2020

Confrontation cultures of embryonic stem cells with multicellular tumor spheroids to study tumor-induced angiogenesis

1 reference

12 December 2020

Eosinophils in a tri-cell multicellular tumor spheroid (MTS)/endothelium complex

1 reference

12 December 2020

Growth of prostatic cancer cells, DU 145, as multicellular spheroids and effects of estramustine

1 reference

12 December 2020

Inhibition of the mammalian target of rapamycin sensitizes U87 xenografts to fractionated radiation therapy.

1 reference

12 December 2020

Lack of multicellular drug resistance observed in human ovarian and prostate carcinoma treated with the proteasome inhibitor PS-341.

1 reference

12 December 2020

Identifiers

DOI

10.1038/NPROT.2008.226

2 references

25 May 2017

Consolidated OpenCitations Corpus – April 2017

Dimensions Publication ID

834412

1013942825

0 references

834412

1 reference

Consolidated OpenCitations Corpus – April 2017

Consolidated OpenCitations Corpus – April 2017

1 reference