Mechanistic analysis of the mitotic kinesin Eg5. (Q24540197)

6 July 2017

Mechanistic analysis of the mitotic kinesin Eg5. (English)

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6 July 2017

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Jared C Cochran

1

1 reference

6 July 2017

Christopher A Sontag

2

1 reference

6 July 2017

Zoltan Maliga

3

1 reference

6 July 2017

Tarun M Kapoor

4

1 reference

6 July 2017

John J Correia

5

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6 July 2017

Susan P Gilbert

6

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6 July 2017

language of work or name

English

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publication date

6 July 2004

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6 July 2017

10

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Journal of Biological Chemistry

inferred from page(s)

published in

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6 July 2017

279

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6 July 2017

38861-38870

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6 July 2017

37

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All kinesin superfamily protein, KIF, genes in mouse and human

6 July 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5.

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Opposing motor activities are required for the organization of the mammalian mitotic spindle pole

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Crystal structure of the kinesin motor domain reveals a structural similarity to myosin

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

A "slow" homotetrameric kinesin-related motor protein purified from Drosophila embryos

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Inhibition of a mitotic motor protein: where, how, and conformational consequences

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

A structural pathway for activation of the kinesin motor ATPase.

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Switch-based mechanism of kinesin motors

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3 A resolution

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

X-ray structure of motor and neck domains from rat brain kinesin

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The molecular motor toolbox for intracellular transport

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Evidence that monastrol is an allosteric inhibitor of the mitotic kinesin Eg5

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Phosphorylation by p34cdc2 protein kinase regulates binding of the kinesin-related motor HsEg5 to the dynactin subunit p150

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The way things move: looking under the hood of molecular motor proteins

24 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

A structural change in the kinesin motor protein that drives motility

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Mitotic spindle organization by a plus-end-directed microtubule motor

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Spindle mechanics and dynamics during mitosis in Drosophila

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

27 September 2017

Kinesin's second step

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

A mechanistic model for the organization of microtubule asters by motor and non-motor proteins in a mammalian mitotic extract.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Modulation of kinesin binding by the C-termini of tubulin

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Inhibition of kinesin motility by ADP and phosphate supports a hand-over-hand mechanism.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

What kinesin does at roadblocks: the coordination mechanism for molecular walking

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Kinesin walks hand-over-hand

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

A force balance model of early spindle pole separation in Drosophila embryos.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Self-organisation and forces in the microtubule cytoskeleton

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The mitotic spindle: a self-made machine

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Eg5 is static in bipolar spindles relative to tubulin: evidence for a static spindle matrix

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The spindle: a dynamic assembly of microtubules and motors

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Mitosis: a history of division

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Kinetics: a tool to study molecular motors

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Spindle assembly in animal cells.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

A method for directly fitting the time derivative of sedimentation velocity data and an alternative algorithm for calculating sedimentation coefficient distribution functions.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The road less traveled: emerging principles of kinesin motor utilization

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Kinetics processivity and the direction of motion of Ncd.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Kinesin hydrolyses one ATP per 8-nm step

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

An improved function for fitting sedimentation velocity data for low-molecular-weight solutes

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Kinetics and motility of the Eg5 microtubule motor

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Purification and characterization of two monomeric kinesin constructs

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Oligomeric structure of bacteriophage T7 DNA primase/helicase proteins.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Pathway of processive ATP hydrolysis by kinesin.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Kinesin ATPase: rate-limiting ADP release

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The role of microtubule assembly dynamics in mitotic force generation and functional organization of living cells

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Kinesin moves by an asymmetric hand-over-hand mechanism

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Loading direction regulates the affinity of ADP for kinesin

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Stepping and stretching. How kinesin uses internal strain to walk processively

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Coupling of kinesin steps to ATP hydrolysis.

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

The rate-limiting step in microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains occurs while bound to the microtubule.

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Drosophila kinesin minimal motor domain expressed in Escherichia coli. Purification and kinetic characterization

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Pre-steady-state kinetics of the microtubule-kinesin ATPase.

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1356567

Interaction of the mitotic inhibitor monastrol with human kinesin Eg5.

30 May 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Crystal structure of the motor domain of the kinesin-related motor ncd.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Alternating site mechanism of the kinesin ATPase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

The role of ATP hydrolysis for kinesin processivity

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Pathway of ADP-stimulated ADP release and dissociation of tethered kinesin from microtubules. Implications for the extent of processivity

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Measuring kinesin's first step

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

A mechanistic model for Ncd directionality

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

The ATPase cross-bridge cycle of the Kar3 motor domain. Implications for single head motility

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Sedimentation studies on the kinesin motor domain constructs K401, K366, and K341.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Expression, purification, ATPase properties, and microtubule-binding properties of the ncd motor domain.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Kinetic mechanism of a monomeric kinesin construct.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M404203200

Alternate fast and slow stepping of a heterodimeric kinesin molecule

21 January 2018

1 reference

12 December 2020

Moving a microtubule may require two heads: a kinetic investigation of monomeric Ncd

1 reference

12 December 2020

Kinetic evidence for low chemical processivity in ncd and Eg5

1 reference

12 December 2020

Kinetic mechanism of monomeric non-claret disjunctional protein (Ncd) ATPase

1 reference

12 December 2020

Pathway of ATP hydrolysis by monomeric and dimeric kinesin

1 reference

12 December 2020

Equilibrium binding studies of non-claret disjunctional protein (Ncd) reveal cooperative interactions between the motor domains

1 reference

12 December 2020

Interacting head mechanism of microtubule-kinesin ATPase

1 reference

12 December 2020

Identifiers

DOI

10.1074/JBC.M404203200

1 reference

6 July 2017

1 reference

6 July 2017

1 reference