Cell-free gene expression: an expanded repertoire of applications (Q91526782)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:31780816%20AND%20SRC:MED&resulttype=core&format=json

23 April 2020

review article

1 reference

title

Cell-free gene expression: an expanded repertoire of applications (English)

1 reference

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23 April 2020

1

1 reference

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23 April 2020

Ashty S Karim

series ordinal

2

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:31780816%20AND%20SRC:MED&resulttype=core&format=json

23 April 2020

Michael C Jewett

series ordinal

3

1 reference

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23 April 2020

publication date

28 November 2019

1 reference

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23 April 2020

published in

Nature Reviews Genetics

1 reference

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23 April 2020

volume

21

1 reference

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23 April 2020

page(s)

151-170

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23 April 2020

issue

3

1 reference

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Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs

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7 January 2021

Cell-free protein production for NMR studies.

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7 January 2021

Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics.

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7 January 2021

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A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology.

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7 January 2021

A sequential expression system for high-throughput functional genomic analysis.

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7 January 2021

Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.

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7 January 2021

Systematic discovery of natural CRISPR-Cas12a inhibitors

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7 January 2021

Cell-free microcompartmentalised transcription-translation for the prototyping of synthetic communication networks

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7 January 2021

Towards synthesis of a minimal cell

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7 January 2021

Genome replication, synthesis, and assembly of the bacteriophage T7 in a single cell-free reaction.

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7 January 2021

Cell-free TXTL synthesis of infectious bacteriophage T4 in a single test tube reaction

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7 January 2021

A vesicle bioreactor as a step toward an artificial cell assembly

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7 January 2021

Artificial Gel-based Organelles for Spatial Organization of Cell-free Gene Expression Reactions

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7 January 2021

DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation.

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7 January 2021

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

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7 January 2021

In vitro gene expression within membrane-free coacervate protocells.

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7 January 2021

Long-lived protein expression in hydrogel particles: towards artificial cells.

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7 January 2021

Artificial photosynthetic cell producing energy for protein synthesis

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7 January 2021

Towards synthetic cells using peptide-based reaction compartments

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7 January 2021

BioBits™ Bright: A fluorescent synthetic biology education kit

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7 January 2021

BioBits™ Explorer: A modular synthetic biology education kit

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7 January 2021

BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts

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7 January 2021

An educational module to explore CRISPR technologies with a cell-free transcription-translation system

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7 January 2021

Paper-based synthetic gene networks

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7 January 2021

PLANT-Dx: A Molecular Diagnostic for Point-of-Use Detection of Plant Pathogens

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7 January 2021

Strand Displacement Probes Combined with Isothermal Nucleic Acid Amplification for Instrument-Free Detection from Complex Samples.

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7 January 2021

Robust strand exchange reactions for the sequence-specific, real-time detection of nucleic acid amplicons.

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7 January 2021

Adapting enzyme-free DNA circuits to the detection of loop-mediated isothermal amplification reactions

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7 January 2021

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

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Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment

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https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Toehold switches: de-novo-designed regulators of gene expression

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Highly sensitive detection of gene expression of an intronless gene: amplification of mRNA, but not genomic DNA by nucleic acid sequence based amplification (NASBA)

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

DNA detection using recombination proteins

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Field-deployable viral diagnostics using CRISPR-Cas13.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Rapid screening of quorum-sensing signal N-acyl homoserine lactones by an in vitro cell-free assay

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Optimizing Cell-Free Biosensors to Monitor Enzymatic Production

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-Free Protein Synthesis Approach to Biosensing hTRβ-Specific Endocrine Disruptors.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

A paper-based, cell-free biosensor system for the detection of heavy metals and date rape drugs

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Point-of-care biomarker quantification enabled by sample-specific calibration

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts of Escherichia coli

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-free production of scFv fusion proteins: an efficient approach for personalized lymphoma vaccines

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Toward Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Rapid recombinant protein expression in cell-free extracts from human blood.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Rapid expression of vaccine proteins for B-cell lymphoma in a cell-free system.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just-add-water cell-free system

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Lyophilized Escherichia coli-based cell-free systems for robust, high-density, long-term storage.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Portable, On-Demand Biomolecular Manufacturing.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Preservation of protein expression systems at elevated temperatures for portable therapeutic production.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Endotoxin-Free E. coli-Based Cell-Free Protein Synthesis: Pre-Expression Endotoxin Removal Approaches for on-Demand Cancer Therapeutic Production

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

A cell-free expression and purification process for rapid production of protein biologics.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Point-of-care production of therapeutic proteins of good-manufacturing-practice quality

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Digital-to-biological converter for on-demand production of biologics

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Improving cell-free protein synthesis through genome engineering of Escherichia coli lacking release factor 1

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Oligonucleotide-mediated tRNA sequestration enables one-pot sense codon reassignment in vitro

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Engineering toward a bacterial "endoplasmic reticulum" for the rapid expression of immunoglobulin proteins

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Production of site-specific antibody-drug conjugates using optimized non-natural amino acids in a cell-free expression system

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Secretion in yeast: reconstitution of the translocation and glycosylation of alpha-factor and invertase in a homologous cell-free system

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

1 reference

7 January 2021

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription-translation system.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-free unnatural amino acid incorporation with alternative energy systems and linear expression templates

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

In silico assessment of cell-free systems

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Metabolic Profiling of Escherichia coli-based Cell-Free Expression Systems for Process Optimization

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Cell-Free Protein Synthesis Enhancement from Real-Time NMR Metabolite Kinetics: Redirecting Energy Fluxes in Hybrid RRL Systems.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

The E. coli S30 lysate proteome: A prototype for cell-free protein production.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Characterization of the all-E. coli transcription-translation system myTXTL by mass spectrometry

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Quantification of Interlaboratory Cell-Free Protein Synthesis Variability

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Increasing PCR fragment stability and protein yields in a cell-free system with genetically modified Escherichia coli extracts.

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

A continuous cell-free translation system capable of producing polypeptides in high yield

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Green fluorescent protein as a marker for gene expression

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Engineering and characterization of a superfolder green fluorescent protein

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry

1 reference

https://api.crossref.org/works/10.1038%2FS41576-019-0186-3

7 January 2021

10.1038/S41576-019-0186-3

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:31780816%20AND%20SRC:MED&resulttype=core&format=json

23 April 2020

1 reference