Escherichia coli SecA helicase activity is not required in vivo for efficient protein translocation or autogenous regulation (Q43690465)

25 November 2017

Escherichia coli SecA helicase activity is not required in vivo for efficient protein translocation or autogenous regulation (English)

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25 November 2017

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M O Schmidt

1

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25 November 2017

R M Brosh

series ordinal

2

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25 November 2017

D B Oliver

series ordinal

3

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25 November 2017

language of work or name

English

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publication date

27 July 2001

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Journal of Biological Chemistry

25 November 2017

10

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based on heuristic

inferred from page(s)

published in

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25 November 2017

276

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25 November 2017

37076-37085

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25 November 2017

40

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Escherichia coli translocase: the unravelling of a molecular machine

25 November 2017

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https://api.crossref.org/works/10.1074%2FJBC.M104584200

In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecYEG assembles into a tetramer to form the active protein translocation channel

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Roles of SecG in ATP- and SecA-dependent protein translocation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The catalytic cycle of the escherichia coli SecA ATPase comprises two distinct preprotein translocation events

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A significant fraction of functional SecA is permanently embedded in the membrane. SecA cycling on and off the membrane is not essential during protein translocation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Dual regulation of Escherichia coli secA translation by distinct upstream elements

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Identification of elements on GeneX-secA RNA of Escherichia coli required for SecA binding and secA auto-regulation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The first gene in the Escherichia coli secA operon, gene X, encodes a nonessential secretory protein

21 January 2018

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https://api.crossref.org/works/10.1074%2FJBC.M104584200

Secretion monitor, SecM, undergoes self-translation arrest in the cytosol

21 January 2018

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https://api.crossref.org/works/10.1074%2FJBC.M104584200

Autogenous translation regulation by Escherichia coli ATPase SecA may be mediated by an intrinsic RNA helicase activity of this protein

21 January 2018

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https://api.crossref.org/works/10.1074%2FJBC.M104584200

RNA helicase activity of Escherichia coli SecA protein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Two distinct ATP-binding domains are needed to promote protein export by Escherichia coli SecA ATPase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Use of T7 RNA polymerase to direct expression of cloned genes

21 January 2018

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https://api.crossref.org/works/10.1074%2FJBC.M104584200

Helicase motifs: the engine that powers DNA unwinding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A point mutation in Escherichia coli DNA helicase II renders the enzyme nonfunctional in two DNA repair pathways. Evidence for initiation of unwinding from a nick in vivo.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Mutation of a highly conserved arginine in motif IV of Escherichia coli DNA helicase II results in an ATP-binding defect.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Helicase motifs V and VI of the Escherichia coli UvrB protein of the UvrABC endonuclease are essential for the formation of the preincision complex.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA proteins of Bacillus subtilis and Escherichia coli possess homologous amino-terminal ATP-binding domains regulating integration into the plasma membrane

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Endogenous SecA catalyzes preprotein translocation at SecYEG.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Isolation and analysis of dominant secA mutations in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Carboxy-terminal region of Escherichia coli SecA ATPase is important to promote its protein translocation activity in vivo.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The molecular chaperone SecB is released from the carboxy-terminus of SecA during initiation of precursor protein translocation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structure of UvrB, a DNA helicase adapted for nucleotide excision repair

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Crystal structure of a DExx box DNA helicase

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Integration of SecA protein into the Escherichia coli inner membrane is regulated by its amino-terminal ATP-binding domain

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Cross-talk between catalytic and regulatory elements in a DEAD motor domain is essential for SecA function

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Characterization of a Mutant Form of SecA That Alleviates a SecY Defect at Low Temperature and Shows a Synthetic Defect with SecY Alteration at High Temperature

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Genetic dissection of SecA: suppressor mutations against the secY205 translocase defect

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Two Independent Mechanisms Down-regulate the Intrinsic SecA ATPase Activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

SecA Is Not Required for Signal Recognition Particle-mediated Targeting and Initial Membrane Insertion of a Nascent Inner Membrane Protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Regulation of a membrane component required for protein secretion in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

A partially functional DNA helicase II mutant defective in forming stable binary complexes with ATP and DNA. A role for helicase motif III

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Site-directed mutations in motif VI of Escherichia coli DNA helicase II result in multiple biochemical defects: evidence for the involvement of motif VI in the coupling of ATPase and DNA binding activities via conformational changes

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

Nucleotide binding activity of SecA homodimer is conformationally regulated by temperature and altered by prlD and azi mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

The high affinity ATP binding site modulates the SecA-precursor interaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M104584200

21 January 2018

Identifiers

DOI

10.1074/JBC.M104584200

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25 November 2017

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