Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris (Q41473721)

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19 February 2020

title

Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris (English)

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19 February 2020

Pichia pastoris

1 reference

1

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19 February 2020

Helmut Schwab

4

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19 February 2020

Anton Glieder

5

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19 February 2020

author name string

Mudassar Ahmad

2

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19 February 2020

Florian W Krainer

3

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19 February 2020

publication date

14 July 2015

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19 February 2020

published in

Microbial Cell Factories

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19 February 2020

volume

14

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19 February 2020

page(s)

103

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https://scigraph.springernature.com/pub.10.1186/s12934-015-0293-6

19 February 2020

exact match

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Regulation of Pichia pastoris promoters and its consequences for protein production

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Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology

11 June 2018

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Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

11 June 2018

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SLiCE: a novel bacterial cell extract-based DNA cloning method

11 June 2018

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Re-engineering multicloning sites for function and convenience

11 June 2018

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High-throughput protein expression using a combination of ligation-independent cloning (LIC) and infrared fluorescent protein (IFP) detection

11 June 2018

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Secretion and proteolysis of heterologous proteins fused to the Escherichia coli maltose binding protein in Pichia pastoris

11 June 2018

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11 June 2018

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Expression in the yeast Pichia pastoris

11 June 2018

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REBASE--a database for DNA restriction and modification: enzymes, genes and genomes

11 June 2018

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Circular polymerase extension cloning of complex gene libraries and pathways

11 June 2018

1 reference

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Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes

11 June 2018

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Enzymatic assembly of DNA molecules up to several hundred kilobases

11 June 2018

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A one pot, one step, precision cloning method with high throughput capability

11 June 2018

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Promoter library designed for fine-tuned gene expression in Pichia pastoris

11 June 2018

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Expression and secretion of recombinant ZZ-EGFP fusion protein by the methylotrophic yeast Pichia pastoris

11 June 2018

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Coevolution of a Homing Endonuclease and Its Host Target Sequence

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris.

11 June 2018

1 reference

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Directed evolution and substrate specificity profile of homing endonuclease I-SceI.

11 June 2018

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Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

11 June 2018

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Fluorescent proteins as tools to aid protein production

11 June 2018

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Seamless cloning and gene fusion.

11 June 2018

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Heterologous protein production using the Pichia pastoris expression system.

11 June 2018

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Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris

11 June 2018

1 reference

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Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

11 June 2018

1 reference

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Reliable high-throughput screening with Pichia pastoris by limiting yeast cell death phenomena

11 June 2018

1 reference

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Viral preprotoxin signal sequence allows efficient secretion of green fluorescent protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Horseradish peroxidase: a modern view of a classic enzyme

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Membrane protein expression and production: effects of polyhistidine tag length and position

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Many type IIs restriction endonucleases interact with two recognition sites before cleaving DNA.

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Yeast secretory expression of insulin precursors

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Optimization of the production of a honeybee odorant-binding protein by Pichia pastoris

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Isolation of the putative structural gene for the lysine-arginine-cleaving endopeptidase required for processing of yeast prepro-alpha-factor

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Secretion and processing of insulin precursors in yeast

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membrane protein overexpression.

11 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Analysis of the 5' untranslated region (5'UTR) of the alcohol oxidase 1 (AOX1) gene in recombinant protein expression in Pichia pastoris

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Hydrophobin signal sequence mediates efficient secretion of recombinant proteins in Pichia pastoris

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

A simple and rapid method for selecting high producers of recombinant proteins in individual clones of P. pastoris.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

A series of novel directional cloning and expression vectors for blunt-end ligation of PCR products.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

An upstream activation sequence controls the expression of AOX1 gene in Pichia pastoris

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Creation of a type IIS restriction endonuclease with a long recognition sequence

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Effective high-throughput overproduction of membrane proteins in Escherichia coli

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Production of bifunctional single-chain antibody-based fusion proteins in Pichia pastoris supernatants

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Laboratory evolved biocatalysts for stereoselective syntheses of substituted benzaldehyde cyanohydrins

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

An efficient plasmid vector for expression cloning of large numbers of PCR fragments in Escherichia coli.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Comparative expression study to increase the solubility of cold adapted Vibrio proteins in Escherichia coli.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Secretion of human interleukin-2 fused with green fluorescent protein in recombinant Pichia pastoris.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Comparison of affinity tags for protein purification

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Secretion of Pem-CMG, a peptide in the CHH/MIH/GIH family of Penaeus monodon, in Pichia pastoris is directed by secretion signal of the alpha-mating factor from Saccharomyces cerevisiae

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Green fluorescent protein as a reporter of human mu-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501187

Enzyme free cloning for high throughput gene cloning and expression

19 August 2018

1 reference

12 December 2020

High-throughput recombinant gene expression systems in Pichia pastoris using newly developed plasmid vectors

1 reference

12 December 2020

A universal method for the direct cloning of PCR amplified nucleic acid

1 reference

12 December 2020

Construction of T-tailed vectors derived from a pUC plasmid: a rapid system for direct cloning of unmodified PCR products

1 reference

12 December 2020

Creating seamless junctions independent of restriction sites in PCR cloning

1 reference

12 December 2020

Monitoring the transport of recombinant Candida rugosa lipase by a green fluorescent protein-lipase fusion

1 reference

12 December 2020

10.1186/S12934-015-0293-6

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26169367%20AND%20SRC:MED&resulttype=core&format=json

Dimensions Publication ID

19 February 2020

1 reference

9 September 2019

https://scigraph.springernature.com/pub.10.1186/s12934-015-0293-6

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26169367%20AND%20SRC:MED&resulttype=core&format=json

19 February 2020

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:26169367%20AND%20SRC:MED&resulttype=core&format=json