Fluorescent-labeled antibodies: Balancing functionality and degree of labeling (Q40744930)

23 September 2017

Fluorescent-labeled antibodies: Balancing functionality and degree of labeling (English)

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23 September 2017

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Shaleen Vira

1

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23 September 2017

Elena Mekhedov

series ordinal

2

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23 September 2017

Glen Humphrey

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3

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23 September 2017

Paul S Blank

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4

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23 September 2017

language of work or name

English

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publication date

31 March 2010

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23 September 2017

Analytical Biochemistry

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23 September 2017

402

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23 September 2017

2

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23 September 2017

146-150

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Fluorescence-quenching and resonance energy transfer studies of lipid microdomains in model and biological membranes

23 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Multiple binding sites of fluorescein isothiocyanate moieties on myoglobin: photophysical heterogeneity as revealed by ground- and excited-state spectroscopy

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

High-resolution FRET microscopy of cholera toxin B-subunit and GPI-anchored proteins in cell plasma membranes

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Single-molecule imaging of EGFR signalling on the surface of living cells

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Identification of fluorescein-5'-isothiocyanate-modification sites in proteins by electrospray-ionization mass spectrometry

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Conjugation of fluorochromes to antibodies

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Kinetics of antigen-antibody reactions at solid-liquid interfaces

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Fluorescence quenching; a practical problem in flow cytometry

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method

24 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

External diffusion in solid-phase immunoassays.

15 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Optimal labeling condition of antibodies available for immunofluorescence endoscopy.

15 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Development and validation of a kinetic assay for analysis of anti-human interleukin-5 monoclonal antibody (SCH 55700) and human interleukin-5 interactions using surface plasmon resonance.

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Antibody-antigen interactions measured by surface plasmon resonance: global fitting of numerical integration algorithms

19 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2876214

Subtype specific ELISA for the detection of antibodies against influenza A H1N1 and H3N2 in swine

19 August 2018

1 reference

12 December 2020

Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin

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12 December 2020

Protein labeling with fluorescent probes

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12 December 2020

Cooperative roles of factor V(a) and phosphatidylserine-containing membranes as cofactors in prothrombin activation

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12 December 2020

The quantitation of nonspecific staining as a guide for improvement of fluorescent antibody conjugates

1 reference

12 December 2020

Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation

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12 December 2020

Conjugation of antibodies with fluorochromes: modifications to the standard methods

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12 December 2020

Labelling of Specific Antibodies with Fluorescein Isothiocyanate with Protection of the Antigen-Binding Site

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12 December 2020

A comparison of fluorescein isothiocyanate and lissamine rhodamine (RB 200) as labels for antibody in the fluorescent antibody technique

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12 December 2020

30 Quantitative, single-tube, kinetic-dependent enzyme-linked immunosorbent assay (k-ELISA)

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12 December 2020

Quality control of fluorescein isothiocyanate-labeled reagents

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12 December 2020

Dissociation of antibodies bound to surface-immobilized antigen

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12 December 2020

Conjugation of fluorochromes to antibodies

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12 December 2020

Coupling of monoclonal antibodies with fluorophores

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12 December 2020

Analysis of fluorescence lifetime and quenching of FITC-conjugated antibodies on cells by phase-sensitive flow cytometry

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12 December 2020

PURIFICATION AND ANALYSIS OF FLUORESCEIN-LABELED ANTISERA BY COLUMN CHROMATOGRAPHY

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12 December 2020

Improved detection of cell death in vivo with annexin V radiolabeled by site-specific methods

1 reference

12 December 2020

Alzheimer's-disease-associated conformation of intrinsically disordered tau protein studied by intrinsically disordered protein liquid-phase competitive enzyme-linked immunosorbent assay

1 reference

12 December 2020

Glycerin suppression of fluorescence self-quenching and improvement of heterogeneous fluoroimmunoassay sensitivity

1 reference

12 December 2020

Parallel kinetic analysis and affinity determination of hundreds of monoclonal antibodies using the ProteOn XPR36

1 reference

12 December 2020

Fluorescein labeling of Fab' while preserving single thiol

1 reference

12 December 2020

Effect of antibody affinity on the isotherm of antibody binding to surface-immobilized antigen

1 reference

12 December 2020

Identifiers

DOI

10.1016/J.AB.2010.03.036

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23 September 2017

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23 September 2017

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