High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9. (Q40429072)

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29 March 2020

title

High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9 (English)

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8

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Francisco Barro

9

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Daniel F Voytas

11

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7

Nicholas J Baltes

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Javier Gil-Humanes

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author name string

Yanpeng Wang

2

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Zhen Liang

3

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Qiwei Shan

4

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Carmen V Ozuna

5

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Susana Sánchez-León

6

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Caixia Gao

10

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publication date

13 February 2017

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volume

89

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issue

6

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page(s)

1251-1262

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Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System

29 March 2020

cites work

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In planta gene targeting

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A geminiviral amplicon (VA) derived from Tomato leaf curl virus (ToLCV) can replicate in a wide variety of plant species and also acts as a VIGS vector

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Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease

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The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.

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Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors

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Precise genome modification in the crop species Zea mays using zinc-finger nucleases

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21 January 2018

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Efficient and stable expression of GFP through Wheat streak mosaic virus-based vectors in cereal hosts using a range of cleavage sites: formation of dense fluorescent aggregates for sensitive virus tracking

21 January 2018

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Trans replication and high copy numbers of wheat dwarf virus vectors in maize cells

21 January 2018

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High-frequency modification of plant genes using engineered zinc-finger nucleases

21 January 2018

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Replication of a geminivirus derived shuttle vector in maize endosperm cells

21 January 2018

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Geminiviruses and RNA silencing

21 January 2018

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TAL effector nucleases induce mutations at a pre-selected location in the genome of primary barley transformants.

21 January 2018

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Enhanced viral intergenic region-specific short interfering RNA accumulation and DNA methylation correlates with resistance against a geminivirus

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21 January 2018

Identifiers

DOI

10.1111/TPJ.13446

1 reference

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29 March 2020

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