Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants. (Q39595874)

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scientific article published on October 1999

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English	Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants.	scientific article published on October 1999

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scholarly article

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12 December 2019

Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: a new approach for construction of HCMV mutants (English)

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12 December 2019

Cytomegalovirus

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Escherichia coli

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inferred from title

Martin Messerle

4

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12 December 2019

author name string

Borst EM

1

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Hahn G

2

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12 December 2019

Koszinowski UH

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publication date

1 October 1999

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12 December 2019

Journal of Virology

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12 December 2019

73

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12 December 2019

8320-8329

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12 December 2019

10

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12 December 2019

Systematic excision of vector sequences from the BAC-cloned herpesvirus genome during virus reconstitution.

1 reference

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A recombinant human cytomegalovirus with a large deletion in UL97 has a severe replication deficiency

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Reassessing the organization of the UL42-UL43 region of the human cytomegalovirus strain AD169 genome.

1 reference

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Defective growth correlates with reduced accumulation of a viral DNA replication protein after low-multiplicity infection by a human cytomegalovirus ie1 mutant

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Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome

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Human cytomegalovirus tegument protein pp71 (ppUL82) enhances the infectivity of viral DNA and accelerates the infectious cycle

1 reference

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A deletion mutant in the human cytomegalovirus gene encoding IE1(491aa) is replication defective due to a failure in autoregulation

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The human cytomegalovirus UL37 immediate-early regulatory protein is an integral membrane N-glycoprotein which traffics through the endoplasmic reticulum and Golgi apparatus

1 reference

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Immortalization of human B lymphocytes by a plasmid containing 71 kilobase pairs of Epstein-Barr virus DNA.

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Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

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Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli

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Characterization of a viable, noninverting herpes simplex virus 1 genome derived by insertion and deletion of sequences at the junction of components L and S.

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Cloning of the complete human cytomegalovirus genome in cosmids

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A generalized technique for deletion of specific genes in large genomes: alpha gene 22 of herpes simplex virus 1 is not essential for growth.

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Selective extraction of polyoma DNA from infected mouse cell cultures

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Herpes simplex virus 1 recombinants with noninverting genomes frozen in different isomeric arrangements are capable of independent replication

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Insertion and deletion mutagenesis of the human cytomegalovirus genome

1 reference

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Construction of large DNA segments in Escherichia coli

1 reference

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14 September 2017

Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169.

1 reference

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Human cytomegalovirus UL36-38 and US3 immediate-early genes: temporally regulated expression of nuclear, cytoplasmic, and polysome-associated transcripts during infection

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Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector

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Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1 cis-acting elements

1 reference

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The published DNA sequence of human cytomegalovirus strain AD169 lacks 929 base pairs affecting genes UL42 and UL43.

1 reference

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Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids

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Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes.

1 reference

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1 reference

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Open reading frames UL44, IRS1/TRS1, and UL36-38 are required for transient complementation of human cytomegalovirus oriLyt-dependent DNA synthesis.

1 reference

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1 reference

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1 reference

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12 December 2020

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12 December 2019

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12 December 2019

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