Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression. (Q39566897)

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scientific article published on August 1998

Language	Label	Description	Also known as
English	Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression.	scientific article published on August 1998

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scholarly article

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:9683473%20AND%20SRC:MED&resulttype=core&format=json

13 November 2019

Species-dependent phenotypes of replication-temperature-sensitive trfA mutants of plasmid RK2: a codon-neutral base substitution stimulates temperature sensitivity by leading to reduced levels of trfA expression (English)

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Europe PubMed Central

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13 November 2019

4

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Valla S

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13 November 2019

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Karunakaran P

1

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13 November 2019

Blatny JM

2

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13 November 2019

Ertesvåg H

3

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13 November 2019

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1 August 1998

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13 November 2019

Journal of Bacteriology

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13 November 2019

180

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13 November 2019

15

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13 November 2019

3793-3798

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13 November 2019

Strategies for achieving high-level expression of genes in Escherichia coli

1 reference

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14 September 2017

DNA sequence requirements for interaction of the RK2 replication initiation protein with plasmid origin repeats

1 reference

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Reduced Rho-dependent transcription termination permits NusA-independent growth of Escherichia coli.

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria

1 reference

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One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution

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14 September 2017

Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers

1 reference

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Plasmid transformation of Escherichia coli and other bacteria

1 reference

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Codon usage tabulated from the GenBank genetic sequence data

1 reference

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Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

1 reference

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14 September 2017

Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans

1 reference

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Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon

1 reference

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The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop mutations

1 reference

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Suppression of cole1 replication properties by the Inc P-1 plasmid RK2 in hybrid plasmids constructed in vitro

1 reference

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25 September 2017

Replication and incompatibility properties of segments of the origin region of replication of the broad host range plasmid RK2.

1 reference

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15 June 2018

Replication of mini RK2 plasmid in extracts of Escherichia coli requires plasmid-encoded protein TrfA and host-encoded proteins DnaA, B, G DNA gyrase and DNA polymerase III.

1 reference

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15 June 2018

The sequence encoding the 43-kilodalton trfA protein is required for efficient replication or maintenance of minimal RK2 replicons in Pseudomonas aeruginosa

1 reference

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Analysis of nonpolar insertion mutations in the trfA gene of IncP plasmid RK2 which affect its broad-host-range property

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Interactions of plasmid-encoded replication initiation proteins with the origin of DNA replication in the broad host range plasmid RK2.

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Isolation and properties of temperature-sensitive mutants of the trfA gene of the broad host range plasmid RK2

1 reference

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Improved broad-host-range RK2 vectors useful for high and low regulated gene expression levels in gram-negative bacteria

1 reference

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Isolation and characterization of DNA-binding mutants of a plasmid replication initiation protein utilizing an in vivo binding assay

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1 reference

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12 December 2020

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