Fibrous proteins: At the crossroads of genetic engineering and biotechnological applications (Q38579143)

4 September 2017

Fibrous proteins: At the crossroads of genetic engineering and biotechnological applications (English)

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4 September 2017

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3

David L Kaplan

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4 September 2017

Sezin Yigit

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PubMed ID

26332660

4 September 2017

Nina Dinjaski

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2

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4 September 2017

1 September 2015

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Biotechnology and Bioengineering

4 September 2017

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4 September 2017

113

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4 September 2017

913-929

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4 September 2017

5

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Use of spider silk fibres as an innovative material in a biocompatible artificial nerve conduit

4 September 2017

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21 January 2018

Silk-based biomaterials

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Preparation and characterization of elastin-like polypeptide scaffolds for local delivery of antibiotics and proteins.

21 January 2018

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Definition of the native and denatured type II collagen binding site for fibronectin using a recombinant collagen system.

21 January 2018

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The influence of specific binding of collagen-silk chimeras to silk biomaterials on hMSC behavior.

21 January 2018

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Purification and characterization of recombinant spider silk expressed in Escherichia coli.

21 January 2018

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Aqueous Processing and Fiber Spinning of Recombinant Spider Silks

21 January 2018

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Protein polymer hydrogels by in situ, rapid and reversible self-gelation.

21 January 2018

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The promotion of angiogenesis by growth factors integrated with ECM proteins through coiled-coil structures

21 January 2018

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Stimuli-induced release of compounds from elastin biomimetic matrix.

21 January 2018

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Recombinant protein expression in Escherichia coli

21 January 2018

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Destabilization of osteogenesis imperfecta collagen-like model peptides correlates with the identity of the residue replacing glycine

21 January 2018

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Hydration structure of a collagen peptide

21 January 2018

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In vitro 3D full-thickness skin-equivalent tissue model using silk and collagen biomaterials

21 January 2018

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Self-aggregation characteristics of recombinantly expressed human elastin polypeptides

21 January 2018

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Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release

21 January 2018

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A thermally responsive biopolymer for intra-articular drug delivery

21 January 2018

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Orientation within the exosporium and structural stability of the collagen-like glycoprotein BclA of Bacillus anthracis

21 January 2018

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Design and production of a chimeric resilin-, elastin-, and collagen-like engineered polypeptide.

21 January 2018

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Conformational stability of collagen relies on a stereoelectronic effect.

21 January 2018

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Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers

21 January 2018

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Molecular structure of the collagen triple helix

21 January 2018

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One-pot Synthesis of Elastin-like Polypeptide Hydrogels with Grafted VEGF-Mimetic Peptides

21 January 2018

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Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications

21 January 2018

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Spider silkglands contain a tissue-specific alanine tRNA that accumulates in vitro in response to the stimulus for silk protein synthesis

21 January 2018

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Procollagen trafficking, processing and fibrillogenesis.

21 January 2018

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The biological production of protein polymers and their use

21 January 2018

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The use of microfiber composites of elastin-like protein matrix reinforced with synthetic collagen in the design of vascular grafts

21 January 2018

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A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry

21 January 2018

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Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers

21 January 2018

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Silk fibroin/poly(carbonate)-urethane as a substrate for cell growth: in vitro interactions with human cells

21 January 2018

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Peptide-based Biopolymers in Biomedicine and Biotechnology

21 January 2018

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Recombinant human elastin-like magnetic microparticles for drug delivery and targeting

21 January 2018

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Nanostructured and thermoresponsive recombinant biopolymer-based microcapsules for the delivery of active molecules

21 January 2018

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Layer-by-layer film growth using polysaccharides and recombinant polypeptides: a combinatorial approach.

21 January 2018

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Increasing silk fibre strength through heterogeneity of bundled fibrils

21 January 2018

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Purification and cytotoxicity of tag-free bioengineered spider silk proteins

21 January 2018

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Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot.

21 January 2018

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Temperature triggered self-assembly of polypeptides into multivalent spherical micelles

21 January 2018

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A DECODER NMR study of backbone orientation in Nephila clavipes dragline silk under varying strain and draw rate.

21 January 2018

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Production of synthetic spider dragline silk protein in Pichia pastoris.

21 January 2018

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Synthetic spider dragline silk proteins and their production in Escherichia coli

21 January 2018

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Cloning of full-length elastin cDNAs from a human skin fibroblast recombinant cDNA library: further elucidation of alternative splicing utilizing exon-specific oligonucleotides

21 January 2018

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Genetically engineered syntheses of tandem repetitive polypeptides consisting of glycine-rich sequence of spider dragline silk.

21 January 2018

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Designing protein-based biomaterials for medical applications

21 January 2018

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In vitro characterization of a collagen scaffold enzymatically cross-linked with a tailored elastin-like polymer

21 January 2018

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Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

21 January 2018

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Engineered protein scaffolds as next-generation antibody therapeutics.

21 January 2018

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Collagen sponges for bone regeneration with rhBMP-2

21 January 2018

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Elastin-like recombinamers: biosynthetic strategies and biotechnological applications

21 January 2018

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Cloning and expression of a collagen-analog-encoding synthetic gene in Escherichia coli.

21 January 2018

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Antimicrobial functionalized genetically engineered spider silk

21 January 2018

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Molecular model for elastin structure and function.

21 January 2018

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A review of combined experimental and computational procedures for assessing biopolymer structure-process-property relationships

21 January 2018

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Evidence from flagelliform silk cDNA for the structural basis of elasticity and modular nature of spider silks

21 January 2018

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RGD modified polymers: biomaterials for stimulated cell adhesion and beyond

21 January 2018

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The use of plasma-activated covalent attachment of early domains of tropoelastin to enhance vascular compatibility of surfaces

21 January 2018

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Design and synthesis of elastin-like polypeptides for an ideal nerve conduit in peripheral nerve regeneration.

21 January 2018

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Expression and purification of an antimicrobial peptide by fusion with elastin-like polypeptides in Escherichia coli

21 January 2018

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Molecular mechanisms of spider silk

21 January 2018

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Biomaterials derived from silk-tropoelastin protein systems

21 January 2018

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Primary structure elements of spider dragline silks and their contribution to protein solubility

21 January 2018

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Novel assembly properties of recombinant spider dragline silk proteins.

21 January 2018

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Production of recombinant human tropoelastin: characterization and demonstration of immunologic and chemotactic activity.

21 January 2018

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Functional fabrication of recombinant human collagen-phosphorylcholine hydrogels for regenerative medicine applications.

21 January 2018

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Testing the utility of rationally engineered recombinant collagen-like proteins for applications in tissue engineering.

21 January 2018

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Mechanism of silk processing in insects and spiders.

21 January 2018

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The effect of a recombinant elastin-mimetic coating of an ePTFE prosthesis on acute thrombogenicity in a baboon arteriovenous shunt.

21 January 2018

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Chemical conjugation of linear and cyclic RGD moieties to a recombinant elastin-mimetic polypeptide--a versatile approach towards bioactive protein hydrogels

21 January 2018

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Catalytic transformation of waste polymers to fuel oil.

21 January 2018

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Elastin as a self-organizing biomaterial: use of recombinantly expressed human elastin polypeptides as a model for investigations of structure and self-assembly of elastin

21 January 2018

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Nanoconfinement controls stiffness, strength and mechanical toughness of beta-sheet crystals in silk.

21 January 2018

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Synthetic biology: applications come of age

21 January 2018

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All-water-based electron-beam lithography using silk as a resist.

21 January 2018

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Amphiphilic protein micelles for targeted in vivo imaging

21 January 2018

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On the use of machine learning to identify topological rules in the packing of beta-strands

21 January 2018

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De novo design of supercharged, unfolded protein polymers, and their assembly into supramolecular aggregates

21 January 2018

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Self-assembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic wounds

21 January 2018

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Hydrazone self-crosslinking of multiphase elastin-like block copolymer networks

21 January 2018

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Microablation of collagen-based substrates for soft tissue engineering

21 January 2018

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Recombinant extracellular matrix-like proteins with repetitive elastin or collagen-like functional motifs

21 January 2018

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Genetic engineering of self-assembled protein hydrogel based on elastin-like sequences with metal binding functionality

21 January 2018

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Procollagen peptidase: an enzyme excising the coordination peptides of procollagen

21 January 2018

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Spider silk fibers spun from soluble recombinant silk produced in mammalian cells

21 January 2018

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Biomedical applications of collagen

21 January 2018

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Preparation and characterization of wet spun silk fibroin/poly(vinyl alcohol) blend filaments.

21 January 2018

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Electrospun nanofibrous scaffolds for controlled release of adeno-associated viral vectors

21 January 2018

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Protein secondary structure and orientation in silk as revealed by Raman spectromicroscopy

21 January 2018

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Construction and characterization of a 2.5-kilobase procollagen clone

21 January 2018

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Current trends in polyhydroxyalkanoates (PHAs) biosynthesis: insights from the recombinant Escherichia coli.

21 January 2018

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Expression and purification of a spider silk protein: a new strategy for producing repetitive proteins.

21 January 2018

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Resilin-based Materials for Biomedical Applications

21 January 2018

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Electrospun protein fibers as matrices for tissue engineering.

21 January 2018

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Structure and properties of silk fibroin-poly(vinyl alcohol) gel.

21 January 2018

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Silk self-assembly mechanisms and control from thermodynamics to kinetics

21 January 2018

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Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering.

21 January 2018

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Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications

21 January 2018

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Single amino acid substitutions in either YhjD or MsbA confer viability to 3-deoxy-d-manno-oct-2-ulosonic acid-depleted Escherichia coli

21 January 2018

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High-strength silk protein scaffolds for bone repair

21 January 2018

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Regenerated cellulose-silk fibroin blends fibers

21 January 2018

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Synthesis and characterization of macroporous thermosensitive hydrogels from recombinant elastin-like polymers.

21 January 2018

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Total synthesis and expression in Escherichia coli of a gene encoding human tropoelastin.

21 January 2018

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Evaluation of cell penetrating peptides fused to elastin-like polypeptide for drug delivery

21 January 2018

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A thermally responsive Tat-elastin-like polypeptide fusion protein induces membrane leakage, apoptosis, and cell death in human breast cancer cells

21 January 2018

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New members of the collagen superfamily

21 January 2018

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Recursive directional ligation by plasmid reconstruction allows rapid and seamless cloning of oligomeric genes

21 January 2018

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Self-assembly of thermally responsive nanoparticles of a genetically encoded peptide polymer by drug conjugation

21 January 2018

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Synthesis and in vitro evaluation of enzymatically cross-linked elastin-like polypeptide gels for cartilaginous tissue repair.

21 January 2018

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Product purification by reversible phase transition following Escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP.

21 January 2018

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Production and purification of a recombinant elastomeric polypeptide, G-(VPGVG)19-VPGV, from Escherichia coli

21 January 2018

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In vitro and in vivo evaluation of recombinant silk-elastinlike hydrogels for cancer gene therapy

21 January 2018

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Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: examples from the elastin-like polypeptide system.

21 January 2018

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Structural determinants of cross-linking and hydrophobic domains for self-assembly of elastin-like polypeptides

21 January 2018

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Attachment and growth of fibroblast cells on silk fibroin

21 January 2018

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Analysis of the conserved N-terminal domains in major ampullate spider silk proteins

21 January 2018

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Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres

21 January 2018

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Fabrication of a reversible protein array directly from cell lysate using a stimuli-responsive polypeptide

21 January 2018

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Expression and characterization of recombinant human type II collagens with low and high contents of hydroxylysine and its glycosylated forms

21 January 2018

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High-level production of human type I collagen in the yeast Pichia pastoris

21 January 2018

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Recombinant human type II collagens with low and high levels of hydroxylysine and its glycosylated forms show marked differences in fibrillogenesis in vitro.

21 January 2018

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Secondary structure formation and LCST behavior of short elastin-like peptides

21 January 2018

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Spider silk-based gene carriers for tumor cell-specific delivery.

21 January 2018

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Silk-based delivery systems of bioactive molecules.

21 January 2018

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Bioengineered silk protein-based gene delivery systems

21 January 2018

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Recombinant collagen and gelatin for drug delivery

21 January 2018

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New opportunities for an ancient material

21 January 2018

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Self-assembled elastin-like polypeptide particles.

21 January 2018

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Industrial systems biology

21 January 2018

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Biomimetic nanofibrous scaffolds: preparation and characterization of chitin/silk fibroin blend nanofibers

21 January 2018

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Unhydroxylated triple helical collagen I produced in transgenic plants provides new clues on the role of hydroxyproline in collagen folding and fibril formation.

21 January 2018

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Tunable, post-translational hydroxylation of collagen Domains in Escherichia coli

21 January 2018

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Construction, cloning, and expression of synthetic genes encoding spider dragline silk

21 January 2018

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Complete recombinant silk-elastinlike protein-based tissue scaffold

21 January 2018

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Self-assembly of genetically engineered spider silk block copolymers

21 January 2018

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Assembly mechanism of recombinant spider silk proteins.

21 January 2018

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Genome-based identification and analysis of collagen-related structural motifs in bacterial and viral proteins

21 January 2018

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N-terminal nonrepetitive domain common to dragline, flagelliform, and cylindriform spider silk proteins

21 January 2018

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Integrin alpha v beta 3 binds a unique non-RGD site near the C-terminus of human tropoelastin

21 January 2018

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Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide

21 January 2018

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Spider silks: recombinant synthesis, assembly, spinning, and engineering of synthetic proteins

21 January 2018

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Production of spider silk proteins in tobacco and potato.

21 January 2018

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Purification of spider silk-elastin from transgenic plants and application for human chondrocyte proliferation.

21 January 2018

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Doxorubicin-loaded silk films: drug-silk interactions and in vivo performance in human orthotopic breast cancer.

21 January 2018

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Construction of a recombinant bacterial plasmid containing a chick pro-alpha2 collagen gene sequence

21 January 2018

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Functionalized silk-based biomaterials for bone formation.

21 January 2018

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Lysine post-translational modifications of collagen

21 January 2018

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Macroscopic fibers self-assembled from recombinant miniature spider silk proteins

21 January 2018

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Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

21 January 2018

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On the conformation of proteins: hydrophobic ordering of strands in beta-pleated sheets.

21 January 2018

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Building Elastin. Incorporation of recombinant human tropoelastin into extracellular matrices using nonelastogenic rat-1 fibroblasts as a source for lysyl oxidase.

21 January 2018

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Reduction-oxidation control of beta-sheet assembly in genetically engineered silk

21 January 2018

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Bioengineered functional brain-like cortical tissue

21 January 2018

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Silk-based resorbable electronic devices for remotely controlled therapy and in vivo infection abatement.

21 January 2018

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Recombinant expression and structural and binding properties of alpha 1(VI) and alpha 2(VI) chains of human collagen type VI

21 January 2018

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Recombinant DNA production of spider silk proteins

21 January 2018

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Production of recombinant human type I procollagen trimers using a four-gene expression system in the yeast Saccharomyces cerevisiae

21 January 2018

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Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion

21 January 2018

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Free energy transduction in polypeptides and proteins based on inverse temperature transitions

21 January 2018

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Biotechnological production of spider-silk proteins enables new applications.

21 January 2018

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21 January 2018

Silk as a Biomaterial

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Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase

21 January 2018

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New insights into elastic fiber assembly

21 January 2018

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Light-controlled graphene-elastin composite hydrogel actuators.

21 January 2018

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Polyhydroxyalkanoates, challenges and opportunities

21 January 2018

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The immobilization of recombinant human tropoelastin on metals using a plasma-activated coating to improve the biocompatibility of coronary stents

21 January 2018

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Silk fibroin spheres as a platform for controlled drug delivery

21 January 2018

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Recombinant protein scaffolds for tissue engineering

21 January 2018

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Molecular self-assembly and nanochemistry: a chemical strategy for the synthesis of nanostructures

21 January 2018

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Engineering the Salmonella type III secretion system to export spider silk monomers

21 January 2018

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Controlling beta-sheet assembly in genetically engineered silk by enzymatic phosphorylation/dephosphorylation

21 January 2018

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21 January 2018

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Tropoelastin: a versatile, bioactive assembly module

21 January 2018

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Genetic engineering of fibrous proteins: spider dragline silk and collagen

21 January 2018

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Novel nanocomposites from spider silk-silica fusion (chimeric) proteins

21 January 2018

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Recombinant protein purification by self-cleaving aggregation tag.

21 January 2018

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Tunable self-assembly of genetically engineered silk--elastin-like protein polymers

21 January 2018

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Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber

21 January 2018

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Chemical gene synthesis: strategies, softwares, error corrections, and applications

21 January 2018

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Expanding the family of collagen proteins: recombinant bacterial collagens of varying composition form triple-helices of similar stability

21 January 2018

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Recombinant minimalist spider wrapping silk proteins capable of native-like fiber formation

21 January 2018

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Structure of a protein superfiber: spider dragline silk

21 January 2018

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Hydroxylation of recombinant human collagen type I alpha 1 in transgenic maize co-expressed with a recombinant human prolyl 4-hydroxylase

21 January 2018

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The application of recombinant human collagen in tissue engineering

21 January 2018

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Design, expression and characterization of collagen-like proteins based on the cell adhesive and crosslinking sequences derived from native collagens

21 January 2018

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Bioengineered silk gene delivery system for nuclear targeting

21 January 2018

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Effect of chitin/silk fibroin nanofibrous bicomponent structures on interaction with human epidermal keratinocytes.

21 January 2018

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Preparation of semi-interpenetrating polymer networks composed of silk fibroin and poloxamer macromer

21 January 2018

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Self-association of streptococcus pyogenes collagen-like constructs into higher order structures

21 January 2018

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Bacterial collagen-like proteins that form triple-helical structures

21 January 2018

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Expression of EGFP-spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield

21 January 2018

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Genetically directed synthesis and spectroscopic analysis of a protein polymer derived from a flagelliform silk sequence.

21 January 2018

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Silk fibroin protein and chitosan polyelectrolyte complex porous scaffolds for tissue engineering applications

21 January 2018

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Acid extraction and purification of recombinant spider silk proteins

21 January 2018

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Evaluation of the antibacterial activity of a special silk textile in the treatment of atopic dermatitis

21 January 2018

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Production of functional inclusion bodies in endotoxin-free Escherichia coli

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/BIT.25820

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4 September 2017

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PubMed ID

26332660