Fluorescence anisotropy: rapid, quantitative assay for protein-DNA and protein-protein interaction. (Q38363140)

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20 October 2019

title

Fluorescence anisotropy: rapid, quantitative assay for protein-DNA and protein-protein interaction (English)

1 reference

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protein-protein interaction

20 October 2019

main subject

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4

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20 October 2019

author name string

T Heyduk

series ordinal

1

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20 October 2019

Y Ma

series ordinal

2

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20 October 2019

H Tang

series ordinal

3

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20 October 2019

language of work or name

English

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publication date

1 January 1996

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20 October 2019

published in

Methods in Enzymology

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20 October 2019

volume

274

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20 October 2019

page(s)

492-503

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Polarization of the fluorescence of macromolecules. I. Theory and experimental method

20 October 2019

cites work

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CAP interacts with RNA polymerase in solution in the absence of promoter DNA.

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Fluorescence anisotropy assays implicate protein-protein interactions in regulating trp repressor DNA binding

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Yeast TATA binding protein interaction with DNA: fluorescence determination of oligomeric state, equilibrium binding, on-rate, and dissociation kinetics

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Substrate binding and turnover by the highly specific I-PpoI endonuclease.

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Nuclear protein CBP is a coactivator for the transcription factor CREB

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Adenoviral ElA-associated protein p300 as a functional homologue of the transcriptional co-activator CBP

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Riding the evanescent wave

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Lactose repressor-operator DNA interactions: kinetic analysis by a surface plasmon resonance biosensor

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Atlas of Fluorescence Spectra and Lifetimes of Dyes Attached to Protein

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Consensus DNA site for the Escherichia coli catabolite gene activator protein (CAP): CAP exhibits a 450-fold higher affinity for the consensus DNA site than for the E. coli lac DNA site

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7 January 2021

Derivatives of CAP Having No Solvent-Accessible Cysteine Residues, or Having a Unique Solvent-Accessible Cysteine Residue at Amino Acid 2 of the Helix-Turn-Helix Motif

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Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP). I. Saturation and alanine-scanning mutagenesis.

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Use of Mono Q high-resolution ion-exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase

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7 January 2021

Coincidence of the promoter and capped 5' terminus of RNA from the adenovirus 2 major late transcription unit.

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Use of T7 RNA polymerase to direct expression of cloned genes

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7 January 2021

Analysis of the thermodynamic linkage of DNA binding and ion binding for dimeric and tetrameric forms of the lac repressor

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7 January 2021

10.1016/S0076-6879(96)74039-9

Identifiers

DOI

1 reference

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20 October 2019

1 reference