Protein translocation in vitro: biochemical characterization of genetically defined translocation components (Q37890390)

24 August 2017

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title

Protein translocation in vitro: biochemical characterization of genetically defined translocation components (English)

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24 August 2017

J Fandl

1

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24 August 2017

P C Tai

series ordinal

2

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24 August 2017

publication date

1 June 1990

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Journal of Bioenergetics and Biomembranes

24 August 2017

published in

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24 August 2017

volume

22

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24 August 2017

issue

3

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24 August 2017

page(s)

369-387

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https://scigraph.springernature.com/pub.10.1007/bf00763173

24 August 2017

exact match

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cites work

Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

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12 December 2020

ATP is essential for protein translocation into Escherichia coli membrane vesicles

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12 December 2020

The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins

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12 December 2020

Homologous plant and bacterial proteins chaperone oligomeric protein assembly

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12 December 2020

Effects of Escherichia coli secB mutations on pre-maltose binding protein conformation and export kinetics.

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12 December 2020

In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli

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12 December 2020

Proton motive force-dependent and -independent protein translocation revealed by an efficient in vitro assay system of Escherichia coli.

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12 December 2020

SecB functions as a cytosolic signal recognition factor for protein export in E. coli.

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12 December 2020

Mechanisms of protein localization

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12 December 2020

Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli.

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12 December 2020

The secY protein can act post-translationally to promote bacterial protein export.

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12 December 2020

Suppressors of the secY24 mutation: identification and characterization of additional ssy genes in Escherichia coli

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12 December 2020

Synthesis and export of the outer membrane lipoprotein in Escherichia coli mutants defective in generalized protein export

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12 December 2020

The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein

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12 December 2020

The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export.

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12 December 2020

Proton motive force is not obligatory for growth of Escherichia coli

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12 December 2020

Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors

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12 December 2020

Temperature-dependent insertion of prolipoprotein into Escherichia coli membrane vesicles and requirements for ATP, soluble factors, and functional SecY protein for the overall translocation process

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12 December 2020

Reconstitution of rapid and asymmetric assembly of M13 procoat protein into liposomes which have bacterial leader peptidase

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12 December 2020

Translocation of pro-OmpA across inner membrane vesicles of Escherichia coli occurs in two consecutive energetically distinct steps.

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12 December 2020

Biochemical studies of bacterial protein export.

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12 December 2020

Regulation of a membrane component required for protein secretion in Escherichia coli

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12 December 2020

Genetic studies on protein export in bacteria

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12 December 2020

Purification of the Escherichia coli secB gene product and demonstration of its activity in an in vitro protein translocation system

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12 December 2020

Protein export in Escherichia coli requires a soluble activity

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12 December 2020

Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli

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12 December 2020

Purification and properties of groE, a host protein involved in bacteriophage assembly

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12 December 2020

Cytosolic factor purified from Escherichia coli is necessary and sufficient for the export of a preprotein and is a homotetramer of SecB

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12 December 2020

Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

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12 December 2020

Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro

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12 December 2020

SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

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12 December 2020

New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

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12 December 2020

The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene

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12 December 2020

Characterization of the inhibitory (epsilon) subunit of the proton-translocating adenosine triphosphatase from Escherichia coli

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12 December 2020

An Escherichia coli mutant with an amino acid alteration within the signal sequence of outer membrane prolipoprotein

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12 December 2020

Requirement of heat-labile cytoplasmic protein factors for posttranslational translocation of OmpA protein precursors into Escherichia coli membrane vesicles

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12 December 2020

Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein.

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12 December 2020

SecA protein autogenously represses its own translation during normal protein secretion in Escherichia coli

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12 December 2020

SecA protein is required for secretory protein translocation into E. coli membrane vesicles.

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12 December 2020

Efficient in vitro translocation into Escherichia coli membrane vesicles of a protein carrying an uncleavable signal peptide. Characterization of the translocation process.

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12 December 2020

Escherichia coli SecB protein associates with exported protein precursors in vivo

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12 December 2020

Effects of nucleotides on ATP-dependent protein translocation into Escherichia coli membrane vesicles

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12 December 2020

Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes

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12 December 2020

Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma

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12 December 2020

Export of protein: a biochemical view

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12 December 2020

Escherichia coli 6S RNA is not essential for growth or protein secretion

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12 December 2020

Alkaline phosphatase and OmpA protein can be translocated posttranslationally into membrane vesicles of Escherichia coli.

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12 December 2020

ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes

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12 December 2020

Secretory protein translocation across membranes—the role of the ‘docking protein’

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12 December 2020

Suppression of growth and protein secretion defects in Escherichia coli secA mutants by decreasing protein synthesis

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12 December 2020

secD, a new gene involved in protein export in Escherichia coli

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12 December 2020

Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants

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12 December 2020

The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

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12 December 2020

A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.

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12 December 2020

prlA-mediated suppression of signal sequence mutations is modulated by the secA gene product of Escherichia coli K-12.

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12 December 2020

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12 December 2020

PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli

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12 December 2020

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

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12 December 2020

SecA protein is directly involved in protein secretion in Escherichia coli

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12 December 2020

The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

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12 December 2020

Protein unfolding and the energetics of protein translocation across biological membranes

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12 December 2020

70K heat shock related proteins stimulate protein translocation into microsomes.

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12 December 2020

Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles

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12 December 2020

Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins

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12 December 2020

Evidence for the involvement of ATP in co-translational protein translocation.

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12 December 2020

In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP

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12 December 2020

Isolation and characterization of a growth-cycle-reflecting, high-molecular-weight protein associated with Escherichia coli ribosomes

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12 December 2020

Suppressor mutations that restore export of a protein with a defective signal sequence

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12 December 2020

Protein secretion in Escherichia coli

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12 December 2020

Mutations in a new gene, secB, cause defective protein localization in Escherichia coli

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12 December 2020

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle

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12 December 2020

Protein translocation across membranes

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12 December 2020

Trigger factor: a soluble protein that folds pro-OmpA into a membrane-assembly-competent form

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12 December 2020

Correlation of competence for export with lack of tertiary structure of the mature species: a study in vivo of maltose-binding protein in E. coli

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12 December 2020

Binding of a soluble factor of Escherichia coli to preproteins does not require ATP and appears to be the first step in protein export

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12 December 2020

A high concentration of SecA allows proton motive force-independent translocation of a model secretory protein into Escherichia coli membrane vesicles.

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12 December 2020

SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles

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12 December 2020

SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli

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12 December 2020

Characterization of the catalytic subunit of an anion pump.

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12 December 2020

Energy-requiring translocation of the OmpA protein and alkaline phosphatase of Escherichia coli into inner membrane vesicles

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12 December 2020

Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro

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12 December 2020

A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature-sensitive secY mutant.

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12 December 2020

On the translocation of proteins across membranes

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12 December 2020

Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope

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12 December 2020

Roles of H+-ATPase and proton motive force in ATP-dependent protein translocation in vitro

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12 December 2020

Molecular mechanisms of protein secretion: the role of the signal sequence

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12 December 2020

E. coli mutant pleiotropically defective in the export of secreted proteins

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12 December 2020

Identifiers

DOI

10.1007/BF00763173

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24 August 2017

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