Heterologous GPCR expression: a bottleneck to obtaining crystal structures (Q36775593)

16 August 2017

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title

Heterologous GPCR expression: a bottleneck to obtaining crystal structures (English)

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G protein-coupled receptor

16 August 2017

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Emily C McCusker

1

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16 August 2017

Steven E Bane

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2

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16 August 2017

Michelle A O'Malley

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3

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16 August 2017

Anne Skaja Robinson

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4

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16 August 2017

31 March 2007

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16 August 2017

Biotechnology Progress

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16 August 2017

23

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16 August 2017

3

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16 August 2017

540-547

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Overexpression of integral membrane proteins for structural studies

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Analysis of the role of N-glycosylation in cell-surface expression and binding properties of angiotensin II type-2 receptor of rat pheochromocytoma cells

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Target validation of G-protein coupled receptors

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Crystal structure of rhodopsin: A G protein-coupled receptor

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Crystal structure of a photoactivated deprotonated intermediate of rhodopsin

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Membrane protein folding and oligomerization: the two-stage model

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Conserved extracellular cysteine pair in the M3 muscarinic acetylcholine receptor is essential for proper receptor cell surface localization but not for G protein coupling

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Structure and function in rhodopsin: the role of asparagine-linked glycosylation

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N-linked glycosylation is required for optimal AT1a angiotensin receptor expression in COS-7 cells

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Co-expression of molecular chaperones does not improve the heterologous expression of mammalian G-protein coupled receptor expression in yeast

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Role of N-glycan-dependent quality control in the cell-surface expression of the AT1 receptor

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Glycosylation of G-protein-coupled receptors for hormones central to normal reproductive functioning: its occurrence and role.

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Expression of the Human Oxytocin Receptor in Baculovirus-Infected Insect Cells: High-Affinity Binding Is Induced by a Cholesterol-Cyclodextrin Complex

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Lipid requirement for mu opioid receptor binding

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Role of sterols in modulating the human mu-opioid receptor function in Saccharomyces cerevisiae

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Expression of functional M2 muscarinic acetylcholine receptor in Escherichia coli

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Engineering of a proteolytically stable human beta 2-adrenergic receptor/maltose-binding protein fusion and production of the chimeric protein in Escherichia coli and baculovirus-infected insect cells

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Purification of recombinant M1 muscarinic acetylcholine receptor

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Expression of human peripheral cannabinoid receptor for structural studies

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Improved membrane isolation in the purification of beta 2-adrenoceptors from transgenic Escherichia coli

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Topological analysis of the human beta 2-adrenergic receptor expressed in Escherichia coli

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NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.

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Expression of human endothelin receptor ETB by Escherichia coli transformants.

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Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli

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Expression and purification of the Saccharomyces cerevisiae alpha-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor

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Expression of CB2 cannabinoid receptor in Pichia pastoris

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Expression and characterization of human CB1 cannabinoid receptor in methylotrophic yeast Pichia pastoris

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Structure and function in rhodopsin: expression of functional mammalian opsin in Saccharomyces cerevisiae

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Exceptional total and functional yields of the human adenosine (A2a) receptor expressed in the yeast Saccharomyces cerevisiae.

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Production of G-protein-coupled receptors in yeast

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Expression and functional purification of a glycosylation deficient version of the human adenosine 2a receptor for structural studies

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The Chaperone Environment at the Cytoplasmic Face of the Endoplasmic Reticulum Can Modulate Rhodopsin Processing and Inclusion Formation

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7 January 2021

GPCR modulation by RAMPs

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7 January 2021

Quality and authenticity of heterologous proteins synthesized in yeast

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A pedestrian guide to membrane protein crystallization

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Specific lipid requirements of membrane proteins--a putative bottleneck in heterologous expression

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How lipids affect the activities of integral membrane proteins

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Recombinant G protein-coupled receptors from expression to renaturation: a challenge towards structure

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Human beta 2-adrenergic receptor produced in stably transformed insect cells is functionally coupled via endogenous GTP-binding protein to adenylyl cyclase

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The luteinizing hormone receptor activates phospholipase C via preferential coupling to Gi2

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Functional characterisation of the Drosophila 5-HTdro1 and 5-HTdro2B serotonin receptors in insect cells: activation of a G(alpha) s-like protein by 5-HTdro1 but lack of coupling to inhibitory G-proteins by 5-HTdro2B.

1 reference

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Lipid composition of Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) insect cells used for baculovirus infection

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Baculovirus as versatile vectors for protein expression in insect and mammalian cells

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Characterization of 16 human G protein-coupled receptors expressed in baculovirus-infected insect cells.

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Parameters influencing human mu opioid receptor over-expression in baculovirus-infected insect cells.

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In vivo reconstitution of dopamine D2S receptor-mediated G protein activation in baculovirus-infected insect cells: preferred coupling to Gi1 versus Gi2.

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Insect cell bioreactors.

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Purification and functional characterization of the human beta 2-adrenergic receptor produced in baculovirus-infected insect cells

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7 January 2021

Characterization of the rat m3 muscarinic acetylcholine receptor produced in insect cells infected with recombinant baculovirus

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Purification and reconstitution of a recombinant human neurokinin-1 receptor

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Expression, purification, and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide. large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells

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Biochemical and pharmacological characterization of the human bradykinin subtype 2 receptor produced in mammalian cells using the Semliki Forest virus system

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Relating surfactant properties to activity and solubilization of the human adenosine a3 receptor

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G(i1) and G(oA) differentially determine kinetic efficacies of agonists for kappa-opioid receptor

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Heterologous expression of G-protein-coupled receptors: human opioid receptors under scrutiny

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The synthesis and high-level expression of a beta2-adrenergic receptor gene in a tetracycline-inducible stable mammalian cell line

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Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line

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Structure and function in rhodopsin: a tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants

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Evaluation of the tetracycline- and ecdysone-inducible systems for expression of neurotransmitter receptors in mammalian cells

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Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells

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A continuous cell-free translation system capable of producing polypeptides in high yield

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Cell-free production and stable-isotope labeling of milligram quantities of proteins

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Cell-free translation systems for protein engineering

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Expression of G protein coupled receptors in a cell-free translational system using detergents and thioredoxin-fusion vectors

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Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system

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The regulatory mechanisms of export trafficking of G protein-coupled receptors.

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7 January 2021

Identifiers

DOI

10.1021/BP060349B

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16 August 2017

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