Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors (Q36644383)

15 August 2017

Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors (English)

1 reference

15 August 2017

1 reference

Charlene Depry

1

1 reference

PMCID

3584185

15 August 2017

Sohum Mehta

series ordinal

2

1 reference

PMCID

3584185

15 August 2017

Jin Zhang

series ordinal

3

1 reference

15 August 2017

9 November 2012

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15 August 2017

1 reference

15 August 2017

465

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15 August 2017

3

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15 August 2017

373-381

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https://scigraph.springernature.com/pub.10.1007/s00424-012-1175-y

15 August 2017

0 references

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Regulation of nuclear PKA revealed by spatiotemporal manipulation of cyclic AMP.

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Parallel tracking of cAMP and PKA signaling dynamics in living cells with FRET-based fluorescent biosensors

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Imaging the coordination of multiple signalling activities in living cells

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Development of an optimized backbone of FRET biosensors for kinases and GTPases

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Intracellular protein target detection by quantum dots optimized for live cell imaging

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Reporting from the field: genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems

30 September 2017

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A fluorescent reporter of AMPK activity and cellular energy stress

30 September 2017

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Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells

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Signaling diversity of PKA achieved via a Ca2+-cAMP-PKA oscillatory circuit

30 September 2017

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Visualization of PKA activity in plasma membrane microdomains

30 September 2017

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Simultaneous visualization of protumorigenic Src and MT1-MMP activities with fluorescence resonance energy transfer.

30 September 2017

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Blue fluorescent cGMP sensor for multiparameter fluorescence imaging

30 September 2017

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Local and long-range reciprocal regulation of cAMP and cGMP in axon/dendrite formation.

30 September 2017

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Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

30 September 2017

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30 September 2017

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Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

30 September 2017

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Coordination of Rho GTPase activities during cell protrusion

30 September 2017

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The role of membrane microdomains in shaping beta2-adrenergic receptor-mediated cAMP dynamics

30 September 2017

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Simultaneous live cell imaging using dual FRET sensors with a single excitation light.

30 September 2017

1 reference

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Protein kinase inhibitors: contributions from structure to clinical compounds

30 September 2017

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Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design

30 September 2017

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A genetically encoded fluorescent sensor of ERK activity

30 September 2017

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Therapeutic protein kinase inhibitors

30 September 2017

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Rapid turnover rate of phosphoinositides at the front of migrating MDCK cells

30 September 2017

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Genetically encoded calcium indicators.

30 September 2017

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Differential patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors

30 September 2017

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Calcium-dependent regulation of protein kinase D revealed by a genetically encoded kinase activity reporter

30 September 2017

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Cell-based indicator to visualize picomolar dynamics of nitric oxide release from living cells

30 September 2017

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Spectral imaging: principles and applications.

30 September 2017

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Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling

30 September 2017

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Live cell imaging of phosphoinositide dynamics with fluorescent protein domains

30 September 2017

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Neutrophil polarization: spatiotemporal dynamics of RhoA activity support a self-organizing mechanism

30 September 2017

1 reference

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Termination of cAMP signals by Ca2+ and G(alpha)i via extracellular Ca2+ sensors: a link to intracellular Ca2+ oscillations

30 September 2017

1 reference

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Signal propagation from membrane messengers to nuclear effectors revealed by reporters of phosphoinositide dynamics and Akt activity

30 September 2017

1 reference

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Cygnets: in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP.

30 September 2017

1 reference

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Interplay of Ca2+ and cAMP signaling in the insulin-secreting MIN6 beta-cell line

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Imaging phosphoinositide dynamics using GFP-tagged protein domains

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Simultaneous real-time detection of initiator- and effector-caspase activation by double fluorescence resonance energy transfer analysis

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Novel single chain cAMP sensors for receptor-induced signal propagation.

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Activity of Rho-family GTPases during cell division as visualized with FRET-based probes

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Spectral imaging and its applications in live cell microscopy

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Multispectral imaging fluorescence microscopy for living cells

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Protein kinases--the major drug targets of the twenty-first century?

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Circularly permuted green fluorescent proteins engineered to sense Ca2+.

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Fluorescent indicators for cyclic GMP based on cyclic GMP-dependent protein kinase Ialpha and green fluorescent proteins

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Role of metallothionein in nitric oxide signaling as revealed by a green fluorescent fusion protein

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Use of fura red as an intracellular calcium indicator in frog skeletal muscle fibers

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Live-cell molecular analysis of Akt activation reveals roles for activation loop phosphorylation

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Design of fluorescence resonance energy transfer (FRET)-based cGMP indicators: a systematic approach

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

A ratiometric expressible FRET sensor for phosphoinositides displays a signal change in highly dynamic membrane structures in fibroblasts

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.

28 June 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

Förster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

A FRET-based probe for epidermal growth factor receptor bound non-covalently to a pair of synthetic amphipathic helixes.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

Spatio-temporal images of growth-factor-induced activation of Ras and Rap1.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

Ras signalling on the endoplasmic reticulum and the Golgi

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3584185

Cyclic AMP Imaging in Adult Cardiac Myocytes Reveals Far-Reaching β 1 -Adrenergic but Locally Confined β 2 -Adrenergic Receptor–Mediated Signaling

20 September 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

Monitoring Agonist-induced Phospholipase C Activation in Live Cells by Fluorescence Resonance Energy Transfer

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

Simultaneous Recording of Multiple Cellular Events by FRET

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00424-012-1175-Y

A genetically encoded, fluorescent indicator for cyclic AMP in living cells

21 January 2018

1 reference

12 December 2020

Visualization of phosphatase activity in living cells with a FRET-based calcineurin activity sensor

1 reference

12 December 2020

Visualization of kinase activity with FRET-based activity biosensors

1 reference

12 December 2020

Fluorescent sensors for rapid monitoring of intracellular cGMP

1 reference

12 December 2020

10.1007/S00424-012-1175-Y

Identifiers

DOI

1 reference

15 August 2017

1 reference

15 August 2017

1 reference

PMCID

3584185