High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding (Q36369721)

10 December 2017

High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding (English)

1 reference

10 December 2017

7

Jennifer A Doudna

0 references

Michelle Wu

object named as

Michelle J Wu

5

0 references

Evan A Boyle

object named as

Evan A Boyle

1

0 references

William Greenleaf

8

object named as

William J Greenleaf

1 reference

10 December 2017

4

Samuel H Sternberg

1 reference

10 December 2017

6

Chantal K Guegler

1 reference

10 December 2017

Johan O L Andreasson

2

1 reference

PMCID

5448226

10 December 2017

Lauren M Chircus

3

1 reference

PMCID

5448226

10 December 2017

language of work or name

English

0 references

publication date

11 May 2017

1 reference

Proceedings of the National Academy of Sciences of the United States of America

10 December 2017

1 reference

10 December 2017

114

1 reference

10 December 2017

5461-5466

1 reference

10 December 2017

21

1 reference

Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9.

10 December 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

CRISPR/Cas9 in Genome Editing and Beyond

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Rationally engineered Cas9 nucleases with improved specificity

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Conformational control of DNA target cleavage by CRISPR-Cas9.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Sequence determinants of improved CRISPR sgRNA design

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Landscape of target:guide homology effects on Cas9-mediated cleavage

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Genome-wide identification of CRISPR/Cas9 off-targets in human genome

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Quantitative analysis of RNA-protein interactions on a massively parallel array reveals biophysical and evolutionary landscapes

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

DNA targeting specificity of RNA-guided Cas9 nucleases

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Direct measurement of DNA affinity landscapes on a high-throughput sequencing instrument

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage.

28 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5448226

21 September 2018

Identifiers

DOI

10.1073/PNAS.1700557114

1 reference

10 December 2017

1 reference

10 December 2017

1 reference

PMCID

5448226