Nucleic acid amplification-based techniques for pathogen detection and identification (Q36260417)

13 August 2017

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title

Nucleic acid amplification-based techniques for pathogen detection and identification (English)

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13 August 2017

Paul T Monis

1

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author name string

Steven Giglio

series ordinal

2

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PubMed ID

16169776

13 August 2017

language of work or name

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19 September 2005

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Infection, Genetics and Evolution

13 August 2017

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13 August 2017

6

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13 August 2017

1

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13 August 2017

2-12

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MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging

13 August 2017

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12 December 2020

Simultaneous amplification and detection of specific DNA sequences

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12 December 2020

Real-time PCR for the detection of Cryptosporidium parvum

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12 December 2020

Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis

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12 December 2020

Real-time quantitative polymerase chain reaction (QPCR) to identify Myxobolus cerebralis in rainbow trout Oncorhynchus mykiss

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12 December 2020

Detection of Cryptosporidium parvum using oligonucleotide-tagged liposomes in a competitive assay format

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12 December 2020

Genotyping of Cryptosporidium parvum with microsatellite markers.

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12 December 2020

Nanodiagnostics: application of nanotechnology in molecular diagnostics

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12 December 2020

Comparison of SYTO9 and SYBR Green I for real-time polymerase chain reaction and investigation of the effect of dye concentration on amplification and DNA melting curve analysis.

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12 December 2020

Molecular biology techniques in parasite ecology

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12 December 2020

Real-time-PCR assay for diagnosis of Entamoeba histolytica infection

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12 December 2020

Quantification of Plasmodium falciparum gametocytes in differential stages of development by quantitative nucleic acid sequence-based amplification.

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12 December 2020

High-resolution genotyping by amplicon melting analysis using LCGreen

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12 December 2020

Development of a real-time PCR assay for detection of Toxoplasma gondii in pig and mouse tissues

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12 December 2020

A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes

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12 December 2020

Cell culture-Taqman PCR assay for evaluation of Cryptosporidium parvum disinfection

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12 December 2020

Mutation detection using ligase chain reaction in passivated silicon-glass microchips and microchip capillary electrophoresis

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12 December 2020

In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes

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12 December 2020

A quantitative method of determining initial amounts of DNA by polymerase chain reaction cycle titration using digital imaging and a novel DNA stain

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12 December 2020

Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR

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12 December 2020

Real-time nucleic acid sequence-based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum

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12 December 2020

Helicase-dependent isothermal DNA amplification

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12 December 2020

Real-time RT-PCR for quantitation of hepatitis C virus RNA.

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12 December 2020

Real-time nucleic acid sequence-based amplification in nanoliter volumes.

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12 December 2020

Detection of enterovirus RNA in cerebrospinal fluid (CSF) using NucliSens EasyQ Enterovirus assay.

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12 December 2020

3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures

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12 December 2020

Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples

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12 December 2020

Real-time monitoring of branched rolling-circle DNA amplification with peptide nucleic acid beacon

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12 December 2020

Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification

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12 December 2020

Evaluation of the BDProbeTec strand displacement amplification assay in comparison with the AMTD II direct test for rapid diagnosis of tuberculosis.

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12 December 2020

Diagnosis and quantification of Theileria sergenti using TaqMan PCR.

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12 December 2020

Multiplex genotyping of PCR products with MassTag-labeled primers

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12 December 2020

Evaluation of a Real-time PCR-based assay using the lightcycler system for detection of Toxoplasma gondii bradyzoite genes in blood specimens from patients with toxoplasmic retinochoroiditis.

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12 December 2020

A universal nucleic acid sequence biosensor with nanomolar detection limits

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12 December 2020

Quantitative detection of the potato cyst nematode, Globodera pallida, and the beet cyst nematode, Heterodera schachtii, using Real-Time PCR with SYBR green I dye.

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12 December 2020

Evaluation of a homemade SYBR green I reaction mixture for real-time PCR quantification of gene expression

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12 December 2020

The use of NASBA for the detection of microbial pathogens in food and environmental samples

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12 December 2020

Real-time PCR for detection and quantitation of leishmania in mouse tissues

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12 December 2020

Decision forest analysis of 61 single nucleotide polymorphisms in a case-control study of esophageal cancer; a novel method

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12 December 2020

Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes

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12 December 2020

Detection of Viable Oocysts ofCryptosporidiumparvumFollowing Nucleic Acid Sequence Based Amplification

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12 December 2020

The ligation amplification reaction (LAR)--amplification of specific DNA sequences using sequential rounds of template-dependent ligation

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12 December 2020

iFRET: an improved fluorescence system for DNA-melting analysis

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12 December 2020

Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study

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12 December 2020

Real-time PCR for detection of Trypanosoma brucei in human blood samples

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12 December 2020

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format

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12 December 2020

Development of a nucleic acid sequence-based amplification assay that uses gag-based molecular beacons to distinguish between human immunodeficiency virus type 1 subtype C and C' infections in Ethiopia

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12 December 2020

Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse Probe System in challenging sequence environment.

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12 December 2020

Detection of dengue viral RNA using a nucleic acid sequence-based amplification assay.

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12 December 2020

Characterization of a thermostable UvrD helicase and its participation in helicase-dependent amplification

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12 December 2020

Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence

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12 December 2020

A new minor groove binding asymmetric cyanine reporter dye for real-time PCR.

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12 December 2020

Identification of Escherichia coli O157:H7 and other enterohemorrhagic serotypes by EHEC- hlyA targeting, strand displacement amplification, and fluorescence polarization.

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12 December 2020

On-chip nanoliter-volume multiplex TaqMan polymerase chain reaction from a single copy based on counting fluorescence released microchambers.

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12 December 2020

Determining liver stage parasite burden by real time quantitative PCR as a method for evaluating pre-erythrocytic malaria vaccine efficacy

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12 December 2020

Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA

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12 December 2020

Amplified restriction fragment length polymorphism in parasite genetics

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12 December 2020

Use of fluorescence resonance energy transfer hybridization probes to evaluate quantitative real-time PCR for diagnosis of ocular toxoplasmosis

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12 December 2020

Detection of bacterial DNA polymerase chain reaction products by matrix-assisted laser desorption/ionization mass spectrometry

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12 December 2020

Transcripts of developmentally regulated Plasmodium falciparum genes quantified by real-time RT-PCR.

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12 December 2020

Evaluation of five diagnostic methods for the detection and quantification of Myxobolus cerebralis

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12 December 2020

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by strand displacement amplification and relevance of the amplification control for use with vaginal swab specimens

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12 December 2020

Diagnostic system for rapid and sensitive differential detection of pathogens

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12 December 2020

Duplex Scorpion primers in SNP analysis and FRET applications

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12 December 2020

Quantitative reverse transcription strand displacement amplification: quantitation of nucleic acids using an isothermal amplification technique

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12 December 2020

Experimental model of congenital toxoplasmosis in guinea-pigs: use of quantitative and qualitative PCR for the study of maternofetal transmission.

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12 December 2020

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication

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12 December 2020

Molecular amplification assays for the detection of flaviviruses

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12 December 2020

Analytical performance and clinical utility of a nucleic acid sequence-based amplification assay for detection of cytomegalovirus infection.

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12 December 2020

Detection of M. tuberculosis DNA using thermophilic strand displacement amplification

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12 December 2020

Development and evaluation of a real-time nucleic acid sequence based amplification assay for rapid detection of influenza A.

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12 December 2020

A homogeneous, ligase-mediated DNA diagnostic test

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12 December 2020

Dual-function stem molecular beacons to assess mRNA expression in AT-rich transcripts of Plasmodium falciparum.

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12 December 2020

Quantification of Leishmania infantum parasites in tissue biopsies by real-time polymerase chain reaction and polymerase chain reaction-enzyme-linked immunosorbent assay.

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12 December 2020

Quantitative analysis of gender-regulated transcripts in the filarial nematode Brugia malayi by real-time RT-PCR.

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12 December 2020

High-throughput SNP genotyping with the Masscode system

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12 December 2020

An RNA transcription-based amplification technique (NASBA) for the detection of viable Salmonella enterica.

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12 December 2020

Diagnostic application of padlock probes--multiplex detection of plant pathogens using universal microarrays

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12 December 2020

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

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12 December 2020

Strand displacement amplification--an isothermal, in vitro DNA amplification technique

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12 December 2020

Short report: surveillance of Leishmania sp. among sand flies in Sicily (Italy) using a fluorogenic real-time polymerase chain reaction

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12 December 2020

Quantitation of HIV-1 by real-time PCR with a unique fluorogenic probe

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12 December 2020

Multi-analyte single-membrane biosensor for the serotype-specific detection of Dengue virus

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12 December 2020

Molecular methods for diagnosis and epidemiological studies of parasitic infections

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12 December 2020

Real time quantitative PCR

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12 December 2020

Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening.

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12 December 2020

Development of a multiplex PCR-ligase detection reaction assay for diagnosis of infection by the four parasite species causing malaria in humans

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12 December 2020

Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples

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12 December 2020

Laser desorption mass spectrometry for microbial DNA analysis

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12 December 2020

Detection, differentiation, and quantitation of pathogenic leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay

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12 December 2020

Multiplex real-time NASBA for monitoring expression dynamics of human cytomegalovirus encoded IE1 and pp67 RNA.

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12 December 2020

Detection of viable Cryptosporidium parvum using DNA-modified liposomes in a microfluidic chip.

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12 December 2020

Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction).

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12 December 2020

Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR

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12 December 2020

Lab-on-a-chip systems for biomedical and environmental monitoring.

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12 December 2020

Nucleic-acid sequence based amplification in the rapid diagnosis of rabies

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12 December 2020

An immunomagnetic separation–real-time PCR method for quantification of Cryptosporidium parvum in water samples

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12 December 2020

Quantitative analysis of ITS2 sequences in trichostrongyle parasites

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12 December 2020

Use of ligase chain reaction for the rapid diagnosis of lymph node tuberculosis

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12 December 2020

Single-step quantitation of DNA in microchip electrophoresis with linear imaging UV detection and fluorescence detection through comigration with a digest

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12 December 2020

Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR.

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12 December 2020

Bordetella pertussis detection by spectrofluorometry using polymerase chain reaction (PCR) and a molecular beacon probe

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12 December 2020

Dimethyl sulfoxide as additive in ready-to-use reaction mixtures for real-time polymerase chain reaction analysis with SYBR Green I dye.

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12 December 2020

Evaluation of a commercial test based on ligase chain reaction for direct detection of Mycobacterium tuberculosis in respiratory specimens.

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12 December 2020

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests

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12 December 2020

Analysis of ligase chain reaction products via matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry

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12 December 2020

Improved specificity for Giardia lamblia cyst quantification in wastewater by development of a real-time PCR method.

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12 December 2020

Coupling multiplex RT-PCR to a gene chip assay for sensitive and semiquantitative detection of severe acute respiratory syndrome-coronavirus.

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12 December 2020

Effects of ethidium bromide and SYBR Green I on different polymerase chain reaction systems

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12 December 2020

Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

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12 December 2020

10.1016/J.MEEGID.2005.08.004

Identifiers

DOI

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13 August 2017

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PubMed ID

16169776