New method for generating deletions and gene replacements in Escherichia coli (Q36181236)

12 August 2017

New method for generating deletions and gene replacements in Escherichia coli (English)

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12 August 2017

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C M Hamilton

1

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12 August 2017

M Aldea

2

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12 August 2017

B K Washburn

3

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12 August 2017

P Babitzke

4

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12 August 2017

S R Kushner

5

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12 August 2017

language of work or name

English

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publication date

1 September 1989

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12 August 2017

Journal of Bacteriology

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12 August 2017

171

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12 August 2017

4617-4622

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12 August 2017

9

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Selection for loss of tetracycline resistance by Escherichia coli.

12 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Replacement and amplification of bacterial genes with sequences altered in vitro

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

DNA repair in Escherichia coli: identification of the uvrD gene product

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Rapid and efficient cosmid cloning

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Positive selection for loss of tetracycline resistance.

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Method for determining whether a gene of Escherichia coli is essential: application to the polA gene

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Extent of host deletions associated with bacteriophage P2-mediated eduction

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

In vivo studies of temperature-sensitive recB and recC mutants

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Construction and characterization of a deletion mutant lacking micF, a proposed regulatory gene for OmpF synthesis in Escherichia coli.

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Identification, cloning, and expression of bolA, an ftsZ-dependent morphogene of Escherichia coli

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Plasmid vector pBR322 and its special-purpose derivatives--a review

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Molecular cloning of the gene for the RNA-processing enzyme RNase III of Escherichia coli

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1, pSC101, R100.1 and RTF-TC

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Recombinant levels of Escherichia coli K-12 mutants deficient in various replication, recombination, or repair genes

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Replication of plasmid pSC101 inEscherichia coli K12: Requirement fordnaA function

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Revised Interpretation of the Origin of the pSC101 Plasmid

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Mutations of temperature sensitivity in R plasmid pSC101

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Multimerization of high copy number plasmids causes instability: Cole 1 encodes a determinant essential for plasmid monomerization and stability

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF.

2 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation.

2 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=210259

In vivo transfer of chromosomal mutations onto multicopy plasmids by transduction with bacteriophage P1

2 July 2018

1 reference

12 December 2020

A general method for the construction of Escherichia coli mutants by homologous recombination and plasmid segregation

1 reference

12 December 2020

Controllable alteration of cell genotype in bacterial cultures using an excision vector

1 reference

12 December 2020

Establishment of Escherichia coli cells with an integrated high copy number plasmid

1 reference

12 December 2020

Maintenance of some ColE1-type plasmids in chemostat culture

1 reference

12 December 2020

10.1128/JB.171.9.4617-4622.1989

Identifiers

DOI

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12 August 2017

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12 August 2017

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