Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. (Q35896845)

11 August 2017

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. (English)

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11 August 2017

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Mudra Hegde

4

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Meagan E Sullender

object named as

Meagan Sullender

3

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Craig B Wilen

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Craig Wilen

9

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Herbert W. Virgin

11

object named as

Herbert W Virgin

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11 August 2017

13

David E Root

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11 August 2017

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John G Doench

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11 August 2017

10

Robert Orchard

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11 August 2017

Nicolo Fusi

2

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11 August 2017

Emma W Vaimberg

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11 August 2017

Katherine F Donovan

6

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11 August 2017

Ian Smith

7

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11 August 2017

Zuzana Tothova

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11 August 2017

Jennifer Listgarten

12

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11 August 2017

18 January 2016

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https://scigraph.springernature.com/pub.10.1038/nbt.3437

11 August 2017

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cites work

Identification and characterization of essential genes in the human genome.

1 reference

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Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

11 July 2018

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Next-generation libraries for robust RNA interference-based genome-wide screens.

11 July 2018

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Sequence determinants of improved CRISPR sgRNA design

11 July 2018

1 reference

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Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains

11 July 2018

1 reference

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CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

11 July 2018

1 reference

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Genetic screens and functional genomics using CRISPR/Cas9 technology

11 July 2018

1 reference

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GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

11 July 2018

1 reference

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MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

11 July 2018

1 reference

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Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

11 July 2018

1 reference

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Easy quantitative assessment of genome editing by sequence trace decomposition

11 July 2018

1 reference

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Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

11 July 2018

1 reference

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Improved vectors and genome-wide libraries for CRISPR screening

11 July 2018

1 reference

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Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.

11 July 2018

1 reference

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Measuring error rates in genomic perturbation screens: gold standards for human functional genomics

11 July 2018

1 reference

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CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

11 July 2018

1 reference

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Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

11 July 2018

1 reference

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Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

11 July 2018

1 reference

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DNA interrogation by the CRISPR RNA-guided endonuclease Cas9.

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Discovery and saturation analysis of cancer genes across 21 tumour types

11 July 2018

1 reference

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Genome-scale CRISPR-Cas9 knockout screening in human cells

11 July 2018

1 reference

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Genetic screens in human cells using the CRISPR-Cas9 system

11 July 2018

1 reference

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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

DNA targeting specificity of RNA-guided Cas9 nucleases

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

RNA-programmed genome editing in human cells

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

A genome-scale RNA interference screen implicates NF1 loss in resistance to RAF inhibition

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

RNA-guided human genome engineering via Cas9

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Multiplex genome engineering using CRISPR/Cas systems

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

TRIAD1 inhibits MDM2-mediated p53 ubiquitination and degradation

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

11 July 2018

1 reference

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A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity

11 July 2018

1 reference

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Fast gapped-read alignment with Bowtie 2

11 July 2018

1 reference

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Molecular signatures database (MSigDB) 3.0.

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

COT drives resistance to RAF inhibition through MAP kinase pathway reactivation

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Clinical efficacy of a RAF inhibitor needs broad target blockade in BRAF-mutant melanoma

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Biopython: freely available Python tools for computational molecular biology and bioinformatics

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Support vector machines and kernels for computational biology

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Molecular mechanism of ADP-ribose hydrolysis by human NUDT5 from structural and kinetic studies

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Multivalent binding of p53 to the STAGA complex mediates coactivator recruitment after UV damage

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

AZD6244 (ARRY-142886), a potent inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase 1/2 kinases: mechanism of action in vivo, pharmacokinetic/pharmacodynamic relationship, and potential for combination in precl

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Acute lymphoblastic leukaemia: a model for the pharmacogenomics of cancer therapy.

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Statistical significance for genomewide studies

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

A murine macrophage cell line, immortalized by v-raf and v-myc oncogenes, exhibits normal macrophage functions

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Microhomology-based choice of Cas9 nuclease target sites.

11 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

E-CRISP: fast CRISPR target site identification.

24 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

MELTING, computing the melting temperature of nucleic acid duplex.

24 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4744125

Tests for comparing elements of a correlation matrix

24 September 2018

1 reference

Crossref

https://api.crossref.org/works/10.1038%2FNBT.3437

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1038/NBT.3437

1 reference

11 August 2017

1 reference

11 August 2017

1 reference