TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR. (Q35690774)

9 August 2017

TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR. (English)

1 reference

9 August 2017

Qian Zhang

1

1 reference

9 August 2017

Jing Wang

2

1 reference

9 August 2017

Fang Deng

3

1 reference

9 August 2017

Zhengjian Yan

4

1 reference

9 August 2017

Yinglin Xia

5

1 reference

9 August 2017

Zhongliang Wang

6

1 reference

9 August 2017

Jixing Ye

7

1 reference

9 August 2017

Youlin Deng

8

1 reference

9 August 2017

Zhonglin Zhang

9

1 reference

9 August 2017

Min Qiao

10

1 reference

9 August 2017

Ruifang Li

11

1 reference

9 August 2017

Sahitya K Denduluri

12

1 reference

9 August 2017

Qiang Wei

13

1 reference

9 August 2017

Lianggong Zhao

14

1 reference

9 August 2017

Shun Lu

15

1 reference

9 August 2017

Xin Wang

16

1 reference

9 August 2017

Shengli Tang

17

1 reference

9 August 2017

Hao Liu

18

1 reference

9 August 2017

Hue H Luu

19

1 reference

9 August 2017

Rex C Haydon

20

1 reference

9 August 2017

Tong-Chuan He

21

1 reference

9 August 2017

Li Jiang

22

1 reference

9 August 2017

language of work or name

English

0 references

publication date

14 July 2015

1 reference

9 August 2017

1 reference

9 August 2017

10

1 reference

9 August 2017

7

1 reference

9 August 2017

e0132666

1 reference

Creative Commons Attribution 4.0 International

9 August 2017

start time

14 July 2015

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

0 references

cites work

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Sustained high level transgene expression in mammalian cells mediated by the optimized piggyBac transposon system

12 July 2018

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Canonical Wnt signaling acts synergistically on BMP9-induced osteo/odontoblastic differentiation of stem cells of dental apical papilla (SCAPs).

12 July 2018

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Bone Morphogenetic Protein (BMP) signaling in development and human diseases

12 July 2018

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Functional characteristics of reversibly immortalized hepatic progenitor cells derived from mouse embryonic liver.

12 July 2018

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Bone morphogenetic protein 2 inhibits the proliferation and growth of human colorectal cancer cells

12 July 2018

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The piggyBac transposon-mediated expression of SV40 T antigen efficiently immortalizes mouse embryonic fibroblasts (MEFs).

12 July 2018

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Reversibly Immortalized Mouse Articular Chondrocytes Acquire Long-Term Proliferative Capability While Retaining Chondrogenic Phenotype

12 July 2018

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Overexpression of Ad5 precursor terminal protein accelerates recombinant adenovirus packaging and amplification in HEK-293 packaging cells

12 July 2018

1 reference

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Characterization of constitutive promoters for piggyBac transposon-mediated stable transgene expression in mesenchymal stem cells (MSCs).

12 July 2018

1 reference

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Adenovirus-mediated efficient gene transfer into cultured three-dimensional organoids

12 July 2018

1 reference

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Adenovirus-mediated gene transfer in mesenchymal stem cells can be significantly enhanced by the cationic polymer polybrene

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Targeting BMP9-promoted human osteosarcoma growth by inactivation of notch signaling

12 July 2018

1 reference

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Bone morphogenetic protein-9 effectively induces osteo/odontoblastic differentiation of the reversibly immortalized stem cells of dental apical papilla

12 July 2018

1 reference

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Crosstalk between Wnt/β-catenin and estrogen receptor signaling synergistically promotes osteogenic differentiation of mesenchymal progenitor cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Destabilization of heterologous proteins mediated by the GSK3β phosphorylation domain of the β-catenin protein

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

The E-F hand calcium-binding protein S100A4 regulates the proliferation, survival and differentiation potential of human osteosarcoma cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Cross-talk between EGF and BMP9 signalling pathways regulates the osteogenic differentiation of mesenchymal stem cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Establishment and characterization of the reversibly immortalized mouse fetal heart progenitors

12 July 2018

1 reference

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Decellularized liver scaffolds effectively support the proliferation and differentiation of mouse fetal hepatic progenitors

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Careful selection of reference genes is required for reliable performance of RT-qPCR in human normal and cancer cell lines

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Wnt signaling in bone formation and its therapeutic potential for bone diseases

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

BMP9-regulated angiogenic signaling plays an important role in the osteogenic differentiation of mesenchymal progenitor cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Primer3--new capabilities and interfaces

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Conditionally immortalized mouse embryonic fibroblasts retain proliferative activity without compromising multipotent differentiation potential

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Evaluation and identification of reliable reference genes for pharmacogenomics, toxicogenomics, and small RNA expression analysis

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Taking qPCR to a higher level: Analysis of CNV reveals the power of high throughput qPCR to enhance quantitative resolution

12 July 2018

1 reference

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The posteriorizing gene Gbx2 is a direct target of Wnt signalling and the earliest factor in neural crest induction

12 July 2018

1 reference

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Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis.

12 July 2018

1 reference

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Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells

12 July 2018

1 reference

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Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references

12 July 2018

1 reference

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The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

12 July 2018

1 reference

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Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

12 July 2018

1 reference

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BMP-9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/beta-catenin signalling

12 July 2018

1 reference

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Hey1 basic helix-loop-helix protein plays an important role in mediating BMP9-induced osteogenic differentiation of mesenchymal progenitor cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Touchdown PCR for increased specificity and sensitivity in PCR amplification

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

S100A6 expression and function in human osteosarcoma

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Twenty-five years of quantitative PCR for gene expression analysis.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Regulation of osteogenic differentiation during skeletal development

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

A protocol for rapid generation of recombinant adenoviruses using the AdEasy system

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Selection and validation of optimal siRNA target sites for RNAi-mediated gene silencing

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Quantification of mRNA using real-time RT-PCR

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Complementary techniques: validation of gene expression data by quantitative real time PCR.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Wnt signaling and human diseases: what are the therapeutic implications?

12 July 2018

1 reference

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Quantitative real-time RT-PCR data analysis: current concepts and the novel "gene expression's CT difference" formula

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

CCN1/Cyr61 is regulated by the canonical Wnt signal and plays an important role in Wnt3A-induced osteoblast differentiation of mesenchymal stem cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

RNA integrity and the effect on the real-time qRT-PCR performance

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Real-time PCR for mRNA quantitation

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Connective tissue growth factor (CTGF) is regulated by Wnt and bone morphogenetic proteins signaling in osteoblast differentiation of mesenchymal stem cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs)

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Elevated expression of axin2 and hnkd mRNA provides evidence that Wnt/beta -catenin signaling is activated in human colon tumors

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Mechanisms of Wnt signaling in development

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Identification of c-MYC as a target of the APC pathway

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

A simplified system for generating recombinant adenoviruses

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Real time quantitative PCR

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Noggin resistance contributes to the potent osteogenic capability of BMP9 in mesenchymal stem cells

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Critical selection of internal control genes for quantitative real-time RT-PCR studies in lipopolysaccharide-stimulated human THP-1 and K562 cells.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Growth hormone synergizes with BMP9 in osteogenic differentiation by activating the JAK/STAT/IGF1 pathway in murine multilineage cells.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Real-time reverse transcription polymerase chain reaction to measure mRNA: use, limitations, and presentation of results.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Linear methods for analysis and quality control of relative expression ratios from quantitative real-time polymerase chain reaction experiments.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

The ongoing evolution of qPCR.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4501803

mRNA quantification by real time TaqMan polymerase chain reaction: validation and comparison with RNase protection

25 September 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/26172450

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

High-resolution semi-quantitative real-time PCR without the use of a standard curve

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/26172450

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/26172450

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1371/JOURNAL.PONE.0132666

1 reference

9 August 2017

0 references

1 reference

9 August 2017

1 reference