Determination of protein-ligand interactions using differential scanning fluorimetry (Q35303994)

7 August 2017

Determination of protein-ligand interactions using differential scanning fluorimetry (English)

1 reference

7 August 2017

4

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4 June 2020

author name string

Mirella Vivoli

series ordinal

1

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4 June 2020

Halina R Novak

series ordinal

2

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4 June 2020

Jennifer A Littlechild

series ordinal

3

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4 June 2020

publication date

13 September 2014

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Journal of Visualized Experiments

7 August 2017

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4 June 2020

issue

91

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4 June 2020

page(s)

51809

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Structural insights into WcbI, a novel polysaccharide-biosynthesis enzyme

4 June 2020

cites work

1 reference

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Learning from our mistakes: the 'unknown knowns' in fragment screening

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Using a Fragment-Based Approach To Target Protein-Protein Interactions

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Characterization of the Burkholderia pseudomallei K96243 capsular polysaccharide I coding region

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Thermal denaturation assays in chemical biology

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Efficiency of hit generation and structural characterization in fragment-based ligand discovery.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Calorimetry as a tool for understanding biomolecular interactions and an aid to drug design

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Development of a differential scanning fluorimetry based high throughput screening assay for the discovery of affinity binders against an anthrax protein

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Differential scanning fluorimetry measurement of protein stability changes upon binding to glycosaminoglycans: a screening test for binding specificity

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Differential scanning fluorimetry as secondary screening platform for small molecule inhibitors of Bcl-XL.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Measurement of nanomolar dissociation constants by titration calorimetry and thermal shift assay - radicicol binding to Hsp90 and ethoxzolamide binding to CAII

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

A quantitative model of thermal stabilization and destabilization of proteins by ligands

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multiangle or static light scattering

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Isothermal titration calorimetry: experimental design, data analysis, and probing macromolecule/ligand binding and kinetic interactions

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Thermofluor-based high-throughput stability optimization of proteins for structural studies

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Announcing the worldwide Protein Data Bank

12 July 2018

1 reference

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High-density miniaturized thermal shift assays as a general strategy for drug discovery

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Identification of a Burkholderia mallei polysaccharide gene cluster by subtractive hybridization and demonstration that the encoded capsule is an essential virulence determinant

12 July 2018

1 reference

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The Hill equation revisited: uses and misuses.

12 July 2018

1 reference

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Thermodynamics of the GTP-GDP-operated conformational switch of selenocysteine-specific translation factor SelB.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

Molecular structure of alpha-D-glucose-1-phosphate cytidylyltransferase from Salmonella typhi

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4692391

26 September 2018

Identifiers

DOI

10.3791/51809

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25285605%20AND%20SRC:MED&resulttype=core&format=json

4 June 2020

1 reference

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4 June 2020

1 reference

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