Comparison of pre-analytical FFPE sample preparation methods and their impact on massively parallel sequencing in routine diagnostics (Q35221918)

7 August 2017

Comparison of pre-analytical FFPE sample preparation methods and their impact on massively parallel sequencing in routine diagnostics (English)

1 reference

7 August 2017

Hans-Ulrich Schildhaus

7

Hans-Ulrich Schildhaus

0 references

Sabine Merkelbach-Bruse

10

Sabine Merkelbach-Bruse

0 references

Michaela Angelika Ihle

4

Michaela Angelika Ihle

0 references

Helen Künstlinger

3

Helen Künstlinger

0 references

Anna-Katharina König

5

Katharina König

1 reference

7 August 2017

2

Jana Fassunke

1 reference

7 August 2017

1

Carina Heydt

1 reference

7 August 2017

6

Lukas Carl Heukamp

1 reference

7 August 2017

8

Margarete Odenthal

1 reference

7 August 2017

Reinhard Büttner

9

1 reference

PubMed ID

25105902

7 August 2017

language of work or name

English

0 references

publication date

8 August 2014

1 reference

7 August 2017

1 reference

7 August 2017

9

1 reference

7 August 2017

e104566

1 reference

7 August 2017

8

1 reference

Creative Commons Attribution 4.0 International

7 August 2017

start time

8 August 2014

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

0 references

cites work

Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAF mutations

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Evaluation of five DNA extraction methods for detection of H. pylori in formalin-fixed paraffin-embedded (FFPE) liver tissue from patients with hepatocellular carcinoma

27 July 2018

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Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies

27 July 2018

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Comparison of clinical targeted next-generation sequence data from formalin-fixed and fresh-frozen tissue specimens

27 July 2018

1 reference

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Multiplex picoliter-droplet digital PCR for quantitative assessment of DNA integrity in clinical samples

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Targeted, high-depth, next-generation sequencing of cancer genes in formalin-fixed, paraffin-embedded and fine-needle aspiration tumor specimens

27 July 2018

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Accuracy in copy number calling by qPCR and PRT: a matter of DNA.

27 July 2018

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Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity

27 July 2018

1 reference

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Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Multicentre validation study of nucleic acids extraction from FFPE tissues.

27 July 2018

1 reference

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Effects of KRAS, BRAF, NRAS, and PIK3CA mutations on the efficacy of cetuximab plus chemotherapy in chemotherapy-refractory metastatic colorectal cancer: a retrospective consortium analysis

27 July 2018

1 reference

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Limited copy number-high resolution melting (LCN-HRM) enables the detection and identification by sequencing of low level mutations in cancer biopsies

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

DNA degradation test predicts success in whole-genome amplification from diverse clinical samples

27 July 2018

1 reference

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The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?

27 July 2018

1 reference

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Effect of fixatives and tissue processing on the content and integrity of nucleic acids

27 July 2018

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Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

A high frequency of sequence alterations is due to formalin fixation of archival specimens

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Development and validation of a clinical cancer genomic profiling test based on massively parallel DNA sequencing

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

DNA qualification workflow for next generation sequencing of histopathological samples

26 September 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods.

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Comparison of targeted next-generation sequencing (NGS) and real-time PCR in the detection of EGFR, KRAS, and BRAF mutations on formalin-fixed, paraffin-embedded tumor material of non-small cell lung carcinoma-superiority of NGS.

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Tackling formalin-fixed, paraffin-embedded tumor tissue with next-generation sequencing

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Evaluation of Maxwell® 16 for automated DNA extraction from whole blood and formalin-fixed paraffin embedded (FFPE) tissue.

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Nucleic acid quantity and quality from paraffin blocks: defining optimal fixation, processing and DNA/RNA extraction techniques.

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Low copy number DNA template can render polymerase chain reaction error prone in a sequence-dependent manner

26 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4126727

Reducing Sequence Artifacts in Amplicon-Based Massively Parallel Sequencing of Formalin-Fixed Paraffin-Embedded DNA by Enzymatic Depletion of Uracil-Containing Templates

26 September 2018

1 reference

Crossref

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0104566

Comparison of the DNA extraction methods for polymerase chain reaction amplification from formalin-fixed and paraffin-embedded tissues

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/25105902

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1371/JOURNAL.PONE.0104566

1 reference

7 August 2017

0 references

1 reference

7 August 2017

1 reference

PubMed ID

25105902