Combining computer algorithms with experimental approaches permits the rapid and accurate identification of T cell epitopes from defined antigens (Q34425066)

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English	Combining computer algorithms with experimental approaches permits the rapid and accurate identification of T cell epitopes from defined antigens	scientific article

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scholarly article

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Europe PubMed Central

2 August 2017

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Europe PubMed Central

Combining computer algorithms with experimental approaches permits the rapid and accurate identification of T cell epitopes from defined antigens (English)

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Europe PubMed Central

2 August 2017

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author name string

Schirle M

1

1 reference

Europe PubMed Central

2 August 2017

Weinschenk T

2

1 reference

Europe PubMed Central

2 August 2017

Stevanović S

3

1 reference

Europe PubMed Central

2 August 2017

publication date

1 November 2001

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Europe PubMed Central

2 August 2017

Journal of Immunological Methods

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Europe PubMed Central

2 August 2017

257

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Europe PubMed Central

2 August 2017

1-2

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Europe PubMed Central

2 August 2017

1-16

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Europe PubMed Central

2 August 2017

Prediction of binding to MHC class I molecules.

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7 January 2021

Phenotypic analysis of antigen-specific T lymphocytes

1 reference

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7 January 2021

A structure-based algorithm to predict potential binding peptides to MHC molecules with hydrophobic binding pockets.

1 reference

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7 January 2021

A new liquid chromatography/tandem mass spectrometric approach for the identification of class I major histocompatibility complex associated peptides that eliminates the need for bioassays

1 reference

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7 January 2021

Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion

1 reference

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7 January 2021

Prediction of MHC class II-binding peptides using an evolutionary algorithm and artificial neural network.

1 reference

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7 January 2021

Induction of anti-tumor cytotoxic T lymphocytes in normal humans using primary cultures and synthetic peptide epitopes

1 reference

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7 January 2021

Presentation of viral antigen controlled by a gene in the major histocompatibility complex

1 reference

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7 January 2021

Predominant naturally processed peptides bound to HLA-DR1 are derived from MHC-related molecules and are heterogeneous in size

1 reference

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7 January 2021

H-2Dd exploits a four residue peptide binding motif

1 reference

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7 January 2021

Two distinct proteolytic processes in the generation of a major histocompatibility complex class I-presented peptide

1 reference

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7 January 2021

Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes.

1 reference

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7 January 2021

A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells

1 reference

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7 January 2021

Reverse ELISPOT assay for clonal analysis of cytokine production I. Enumeration of gamma-interferon-secreting cells

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7 January 2021

A computer program for predicting possible cytotoxic T lymphocyte epitopes based on HLA class I peptide-binding motifs

1 reference

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7 January 2021

An empirical method for the prediction of T-cell epitopes.

1 reference

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7 January 2021

HLA class I binding motifs derived from random peptide libraries differ at the COOH terminus from those of eluted peptides

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7 January 2021

An interactive Web site providing major histocompatibility ligand predictions: application to HIV research

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7 January 2021

Homozygous human TAP peptide transporter mutation in HLA class I deficiency

1 reference

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7 January 2021

T-cell antigenic sites tend to be amphipathic structures

1 reference

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7 January 2021

Endogenous peptides bound to HLA-A3 possess a specific combination of anchor residues that permit identification of potential antigenic peptides.

1 reference

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7 January 2021

Accessing complexity: the dynamics of virus-specific T cell responses.

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7 January 2021

HLA-DR-promiscuous T cell epitopes from Plasmodium falciparum pre-erythrocytic-stage antigens restricted by multiple HLA class II alleles

1 reference

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7 January 2021

Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules

1 reference

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7 January 2021

Pool sequencing of natural HLA-DR, DQ, and DP ligands reveals detailed peptide motifs, constraints of processing, and general rules

1 reference

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7 January 2021

The use of human leucocyte antigen class I transgenic mice to investigate human immune function

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New ligands binding to the human leukocyte antigen class II molecule DRB1*0101 based on the activity pattern of an undecapeptide library

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7 January 2021

Induction and exhaustion of lymphocytic choriomeningitis virus-specific cytotoxic T lymphocytes visualized using soluble tetrameric major histocompatibility complex class I-peptide complexes.

1 reference

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7 January 2021

Cell lines transfected with the TAP inhibitor ICP47 allow testing peptide binding to a variety of HLA class I molecules

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An ELISA-based assay for quantitation of human interleukin 2

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7 January 2021

Two complementary methods for predicting peptides binding major histocompatibility complex molecules.

1 reference

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7 January 2021

High-affinity binding of short peptides to major histocompatibility complex class II molecules by anchor combinations

1 reference

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7 January 2021

Precise prediction of major histocompatibility complex class II-peptide interaction based on peptide side chain scanning

1 reference

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7 January 2021

Peptide binding specificity of HLA-DR4 molecules: correlation with rheumatoid arthritis association

1 reference

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7 January 2021

Real time quantitative PCR

1 reference

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Detection and quantification of blood-derived CD8+ T lymphocytes secreting tumor necrosis factor alpha in response to HLA-A2.1-binding melanoma and viral peptide antigens

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7 January 2021

A theoretical approach towards the identification of cleavage-determining amino acid motifs of the 20 S proteasome.

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Neural network-based prediction of candidate T-cell epitopes.

1 reference

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7 January 2021

Characterization of peptides bound to the class I MHC molecule HLA-A2.1 by mass spectrometry

1 reference

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7 January 2021

Peptides presented to the immune system by the murine class II major histocompatibility complex molecule I-Ad

1 reference

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7 January 2021

Enhanced binding of peptide antigen to purified class II major histocompatibility glycoproteins at acidic pH.

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7 January 2021

Detection of intracellular cytokines by flow cytometry

1 reference

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Real-time quantitative polymerase chain reaction assessment of immune reactivity in melanoma patients after tumor peptide vaccination

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Efficient identification of novel HLA-A(*)0201-presented cytotoxic T lymphocyte epitopes in the widely expressed tumor antigen PRAME by proteasome-mediated digestion analysis.

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Use of soluble peptide-DR4 tetramers to detect synovial T cells specific for cartilage antigens in patients with rheumatoid arthritis

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Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood

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7 January 2021

Human immunodeficiency virus type 1 envelope epitope-specific CD4(+) T lymphocytes in simian/human immunodeficiency virus-infected and vaccinated rhesus monkeys detected using a peptide-major histocompatibility complex class II tetramer

1 reference

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7 January 2021

HLA-DQ tetramers identify epitope-specific T cells in peripheral blood of herpes simplex virus type 2-infected individuals: direct detection of immunodominant antigen-responsive cells

1 reference

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Cytotoxic T lymphocytes specific for the simian immunodeficiency virus

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Host resistance directed selectively against H-2-deficient lymphoma variants. Analysis of the mechanism

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7 January 2021

Expression monitoring by hybridization to high-density oligonucleotide arrays

1 reference

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Class II MHC quantitative binding motifs derived from a large molecular database with a versatile iterative stepwise discriminant analysis meta-algorithm

1 reference

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7 January 2021

Melanoma cells present a MAGE-3 epitope to CD4(+) cytotoxic T cells in association with histocompatibility leukocyte antigen DR11.

1 reference

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7 January 2021

Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix

1 reference

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7 January 2021

Expression and kinetics of cytokines determined by intracellular staining using flow cytometry.

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A sensitive proliferation assay to determine the specific T cell response against HLA-A2.1-binding peptides

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7 January 2021

Direct enumeration of Borrelia-reactive CD4 T cells ex vivo by using MHC class II tetramers

1 reference

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7 January 2021

Application of an artificial neural network to predict specific class I MHC binding peptide sequences

1 reference

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7 January 2021

Quantification of antigen specific CD8+ T cells using an ELISPOT assay.

1 reference

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7 January 2021

Selectivity of MHC-encoded peptide transporters from human, mouse and rat

1 reference

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7 January 2021

Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection.

1 reference

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MHC class II tetramers identify peptide-specific human CD4(+) T cells proliferating in response to influenza A antigen

1 reference

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7 January 2021

Cleavage motifs of the yeast 20S proteasome beta subunits deduced from digests of enolase 1.

1 reference

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PAProC: a prediction algorithm for proteasomal cleavages available on the WWW.

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7 January 2021

A quantitative assay to measure the interaction between immunogenic peptides and purified class I major histocompatibility complex molecules

1 reference

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7 January 2021

Flow cytometric measurement of intracellular cytokines.

1 reference

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7 January 2021

Precise prediction of a dominant class I MHC-restricted epitope of Listeria monocytogenes

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SYFPEITHI: database for MHC ligands and peptide motifs

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Distinct T cell interactions with HLA class II tetramers characterize a spectrum of TCR affinities in the human antigen-specific T cell response

1 reference

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Exact prediction of a natural T cell epitope

1 reference

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7 January 2021

Truncation variants of peptides isolated from MHC class II molecules suggest sequence motifs

1 reference

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7 January 2021

Prominent role of secondary anchor residues in peptide binding to HLA-A2.1 molecules

1 reference

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Detection of individual interleukin 4- and gamma interferon-producing murine spleen cells after activation with T-cell mitogens

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Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure

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7 January 2021

Identification of tumor-associated MHC class I ligands by a novel T cell-independent approach

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Evaluation of the interferon-gamma ELISPOT-assay for quantification of peptide specific T lymphocytes from peripheral blood

1 reference

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Structure-based prediction of binding peptides to MHC class I molecules: application to a broad range of MHC alleles

1 reference

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The HLA-A*6601 peptide motif: prediction by pocket structure and verification by peptide analysis

1 reference

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Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysis

1 reference

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7 January 2021

Peptide binding to the most frequent HLA-A class I alleles measured by quantitative molecular binding assays

1 reference

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HLA class II transgenic mice: models of the human CD4+ T-cell immune response.

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Generation of the vesicular stomatitis virus nucleoprotein cytotoxic T lymphocyte epitope requires proteasome-dependent and -independent proteolytic activities

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Peptide binding specificity of major histocompatibility complex class I resolved into an array of apparently independent subspecificities: quantitation by peptide libraries and improved prediction of binding.

1 reference

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7 January 2021

Assessment of major histocompatibility complex class I interaction with Epstein-Barr virus and human immunodeficiency virus peptides by elevation of membrane H-2 and HLA in peptide loading-deficient cells

1 reference

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Identification of wild-type and mutant p53 peptides binding to HLA-A2 assessed by a peptide loading-deficient cell line assay and a novel major histocompatibility complex class I peptide binding assay

1 reference

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7 January 2021

Generation of tissue-specific and promiscuous HLA ligand databases using DNA microarrays and virtual HLA class II matrices

1 reference

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7 January 2021

Identification of a new HLA-A(*)0201-restricted T-cell epitope from the tyrosinase-related protein 2 (TRP2) melanoma antigen

1 reference

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Discrete cleavage motifs of constitutive and immunoproteasomes revealed by quantitative analysis of cleavage products

1 reference

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7 January 2021

A europium fluoroimmunoassay for measuring binding of antigen to class II MHC glycoproteins

1 reference

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7 January 2021

Identification of a MHC class II-restricted human gp100 epitope using DR4-IE transgenic mice

1 reference

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7 January 2021

An automated prediction of MHC class I-binding peptides based on positional scanning with peptide libraries

1 reference

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An HLA class I peptide-binding assay based on competition for binding to class I molecules on intact human B cells. Identification of conserved HIV-1 polymerase peptides binding to HLA-A*0301

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Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA

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7 January 2021

Enumeration of IFN-gamma-producing human lymphocytes by spot-ELISA. A method to detect lymphokine-producing lymphocytes at the single-cell level

1 reference

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7 January 2021

The telomerase catalytic subunit is a widely expressed tumor-associated antigen recognized by cytotoxic T lymphocytes

1 reference

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7 January 2021

Melan-A/MART-1(51-73) represents an immunogenic HLA-DR4-restricted epitope recognized by melanoma-reactive CD4(+) T cells

1 reference

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Flow-cytometric determination of peptide-class I complex formation. Identification of p53 peptides that bind to HLA-A2.

1 reference

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7 January 2021

Identifiers

10.1016/S0022-1759(01)00459-8

1 reference

Europe PubMed Central

2 August 2017

1 reference

Europe PubMed Central

2 August 2017

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