The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins (Q34004711)

30 July 2017

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins (English)

1 reference

30 July 2017

Arne Skerra

2

0 references

author name string

Thomas G M Schmidt

series ordinal

1

1 reference

30 July 2017

1 January 2007

1 reference

30 July 2017

1 reference

30 July 2017

2

1 reference

30 July 2017

1528-1535

1 reference

30 July 2017

6

1 reference

https://scigraph.springernature.com/pub.10.1038/nprot.2007.209

30 July 2017

0 references

cites work

Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Genetically engineered avidins and streptavidins.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Making the most of affinity tags

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Two-step metal affinity purification of double-tagged (NusA-His6) fusion proteins.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

A single step purification for recombinant proteins Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP‐dependent protein kinase

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Assessment of prokaryotic collagen-like sequences derived from streptococcal Scl1 and Scl2 proteins as a source of recombinant GXY polymers

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Comparison of affinity tags for protein purification

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Exploiting features of adenovirus replication to support mammalian kinase production

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Single-step Strep-tag purification for the isolation and identification of protein complexes from mammalian cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitope.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Reversible MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Crystal structure and ligand-binding studies of a screened peptide complexed with streptavidin

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Biotin carboxyl carrier protein co-purifies as a contaminant in core-streptavidin preparations

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

31 March 2020

Identifiers

DOI

10.1038/NPROT.2007.209

1 reference

PubMed ID

17571060

Dimensions Publication ID

30 July 2017

0 references

1 reference

PubMed ID

17571060