The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing. (Q33911870)

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2 February 2020

title

The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing (English)

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2 February 2020

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2

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2 February 2020

Glen Reid

5

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2 February 2020

author name string

Mike Herbert

1

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2 February 2020

Annette Lasham

3

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2 February 2020

Helen Cao

4

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2 February 2020

publication date

25 May 2011

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2 February 2020

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2 February 2020

volume

4

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2 February 2020

page(s)

148

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Creative Commons Attribution 2.0 Generic

2 February 2020

copyright license

start time

25 May 2011

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April 2022 Public Data File from Crossref

copyright status

copyrighted

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April 2022 Public Data File from Crossref

exact match

https://scigraph.springernature.com/pub.10.1186/1756-0500-4-148

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cites work

Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Overexpression of microRNA miR-30a or miR-191 in A549 lung cancer or BEAS-2B normal lung cell lines does not alter phenotype

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

The potency of siRNA-mediated growth inhibition following silencing of essential genes is dependent on siRNA design and varies with target sequence

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5' RACE and a molecular beacon probe

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Potent subunit-specific effects on cell growth and drug sensitivity from optimised siRNA-mediated silencing of ribonucleotide reductase.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

siRNA and innate immunity

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Knocking down barriers: advances in siRNA delivery

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Misinterpreting the therapeutic effects of small interfering RNA caused by immune stimulation

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Sequence- and target-independent angiogenesis suppression by siRNA via TLR3

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Running interference: prospects and obstacles to using small interfering RNAs as small molecule drugs

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Progress towards in vivo use of siRNAs

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Importance of quantitative PCR primer location for short interfering RNA efficacy determination

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3123217

Innate immunity confounds the clinical efficacy of small interfering RNAs (siRNAs).

5 July 2018

1 reference

Crossref

https://api.crossref.org/works/10.1186%2F1756-0500-4-148

RNA interference: PCR strategies for the quantification of stable degradation-fragments derived from siRNA-targeted mRNAs.

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1186%2F1756-0500-4-148

21 January 2018

Identifiers

DOI

10.1186/1756-0500-4-148

1 reference

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Dimensions Publication ID

2 February 2020

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https://scigraph.springernature.com/pub.10.1186/1756-0500-4-148

9 September 2019

1 reference

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2 February 2020

PubMed publication ID

21612654

1 reference