Robust RT-qPCR data normalization: validation and selection of internal reference genes during post-experimental data analysis (Q33851389)

29 July 2017

Robust RT-qPCR data normalization: validation and selection of internal reference genes during post-experimental data analysis (English)

1 reference

29 July 2017

0 references

1 reference

Daijun Ling

1

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PubMed ID

21423626

29 July 2017

Paul M Salvaterra

series ordinal

2

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PubMed ID

21423626

29 July 2017

language of work or name

English

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publication date

15 March 2011

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29 July 2017

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29 July 2017

6

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29 July 2017

3

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29 July 2017

e17762

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Creative Commons Attribution 4.0 International

29 July 2017

start time

15 March 2011

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

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cites work

A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Why the need for qPCR publication guidelines?--The case for MIQE.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Identification and validation of housekeeping genes in brains of the desert locust Schistocerca gregaria under different developmental conditions

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Abeta42-induced neurodegeneration via an age-dependent autophagic-lysosomal injury in Drosophila

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCR.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Using FlyAtlas to identify better Drosophila melanogaster models of human disease

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Quantification of mRNA using real-time RT-PCR

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Real-time RT-PCR normalisation; strategies and considerations.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Guideline to reference gene selection for quantitative real-time PCR

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

The locus elav of Drosophila melanogaster is expressed in neurons at all developmental stages.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

The Drosophila transcript encoded by the beta-amyloid protein precursor-like gene is restricted to the nervous system.

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Identification, secretion, and neural expression of APPL, a Drosophila protein similar to human amyloid protein precursor

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Characterization and spatial distribution of the ELAV protein duringDrosophila melanogaster development

5 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

Evaluation of candidate reference genes for expression studies in Pisum sativum under different experimental conditions

5 July 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0017762

Evaluation of reference genes for quantitative real-time PCR to investigate protein disulfide isomerase transcription pattern in the bovine lungworm Dictyocaulus viviparus

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0017762

Brain aging and Aβ₁₋₄₂ neurotoxicity converge via deterioration in autophagy-lysosomal system: a conditional Drosophila model linking Alzheimer's neurodegeneration with aging.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0017762

Tauopathy in Drosophila: neurodegeneration without neurofibrillary tangles

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0017762

Validation of housekeeping genes for normalizing RNA expression in real-time PCR.

21 January 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3058000

10.1371/JOURNAL.PONE.0017762

29 October 2018

Identifiers

DOI

1 reference

29 July 2017

0 references

1 reference

29 July 2017

1 reference

PubMed ID

21423626