Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture (Q33522558)

27 July 2017

Protocol for the selection of single-domain antibody fragments by third generation intracellular antibody capture (English)

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27 July 2017

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Terence H Rabbitts

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author name string

Tomoyuki Tanaka

series ordinal

1

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27 July 2017

17 December 2009

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27 July 2017

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27 July 2017

5

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1

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27 July 2017

67-92

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https://scigraph.springernature.com/pub.10.1038/nprot.2009.199

27 July 2017

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cites work

Continuous cultures of fused cells secreting antibody of predefined specificity

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Intracellular antibodies and challenges facing their use as therapeutic agents

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Tumour prevention by a single antibody domain targeting the interaction of signal transduction proteins with RAS

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Naturally occurring antibodies devoid of light chains

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Structural analysis of the nurse shark (new) antigen receptor (NAR): molecular convergence of NAR and unusual mammalian immunoglobulins

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Isolation of the new antigen receptor from wobbegong sharks, and use as a scaffold for the display of protein loop libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Single domain intracellular antibodies: a minimal fragment for direct in vivo selection of antigen-specific intrabodies

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Camelized rabbit-derived VH single-domain intrabodies against Vif strongly neutralize HIV-1 infectivity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Human single-domain neutralizing intrabodies directed against Etk kinase: a novel approach to impair cellular transformation

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Identification of single-domain, Bax-specific intrabodies that confer resistance to mammalian cells against oxidative-stress-induced apoptosis

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Llama-derived single-domain intrabodies inhibit secretion of hepatitis B virions in mice

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

A novel genetic system to detect protein-protein interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Intrabodies based on intracellular capture frameworks that bind the RAS protein with high affinity and impair oncogenic transformation

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

An antibody inhibitor of the LMO2-protein complex blocks its normal and tumorigenic functions

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Making antibodies by phage display technology

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Antibody arrays for high-throughput screening of antibody-antigen interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Screening isolates from antibody phage-display libraries

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

De novo production of diverse intracellular antibody libraries

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Mammalian Ras interacts directly with the serine/threonine kinase Raf

21 January 2018

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https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Selection of antibodies for intracellular function using a two-hybrid in vivo system

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Monoclonal antibody successes in the clinic

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Prevention of oculopharyngeal muscular dystrophy-associated aggregation of nuclear poly(A)-binding protein with a single-domain intracellular antibody

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

GAL4 fusion vectors for expression in yeast or mammalian cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.199

31 March 2020

Identifiers

DOI

10.1038/NPROT.2009.199

1 reference

PubMed ID

20057382

Dimensions Publication ID

27 July 2017

0 references

1 reference

PubMed ID

20057382