Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase (Q31056280)

13 September 2017

Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase (English)

1 reference

13 September 2017

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5

Peter G Schultz

1 reference

13 September 2017

Gang Xia

1

1 reference

13 September 2017

Liangjing Chen

2

1 reference

13 September 2017

Takashi Sera

3

1 reference

13 September 2017

Ming Fa

4

1 reference

13 September 2017

Floyd E Romesberg

6

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13 September 2017

language of work or name

English

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publication date

1 May 2002

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Proceedings of the National Academy of Sciences of the United States of America

13 September 2017

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13 September 2017

99

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13 September 2017

10

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13 September 2017

6597-6602

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Directed evolution of polymerase function by compartmentalized self-replication

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Multiple amino acid substitutions allow DNA polymerases to synthesize RNA.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Man-made enzymes--from design to in vitro compartmentalisation

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

DNA polymerase active site is highly mutable: evolutionary consequences

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

A method for directed evolution and functional cloning of enzymes

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Replication defect of moloney murine leukemia virus with a mutant reverse transcriptase that can incorporate ribonucleotides and deoxyribonucleotides

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

RNA-peptide fusions for the in vitro selection of peptides and proteins

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Choosing the right sugar: how polymerases select a nucleotide substrate

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Structural and mechanistic relationships between nucleic acid polymerases

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Rapid evolution of a protein in vitro by DNA shuffling

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Structures of DNA and RNA polymerases and their interactions with nucleic acid substrates

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

The regulation of transcription initiation in bacteria

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Making antibody fragments using phage display libraries

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Complete mutagenesis of protein coding domains

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Footprinting analysis of mammalian RNA polymerase II along its transcript: an alternative view of transcription elongation

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Randomization of genes by PCR mutagenesis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

A ribo-deoxyribonucleotide primer synthesized by primase

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=124448

Cloning of random-sequence oligodeoxynucleotides

21 June 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.102577799

Low fidelity mutants in the O-helix of Thermus aquaticus DNA polymerase I.

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.102577799

A method for the selection of catalytic activity using phage display and proximity coupling.

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.102577799

Efforts toward expansion of the genetic alphabet: replication of DNA with three base pairs

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.102577799

The conserved active site motif A of Escherichia coli DNA polymerase I is highly mutable

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.102577799

A single highly mutable catalytic site amino acid is critical for DNA polymerase fidelity

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.102577799

In situ neutralization in Boc-chemistry solid phase peptide synthesis. Rapid, high yield assembly of difficult sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1073%2FPNAS.102577799

21 January 2018

Identifiers

DOI

10.1073/PNAS.102577799

1 reference

13 September 2017

0 references

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13 September 2017

1 reference