A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts (Q30414621)

15 August 2017

A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts (English)

1 reference

15 August 2017

2

Kevin A Janes

1 reference

15 August 2017

Anjun K Bose

1

1 reference

15 August 2017

11 December 2012

1 reference

Molecular & Cellular Proteomics

15 August 2017

1 reference

15 August 2017

12

1 reference

15 August 2017

3

1 reference

15 August 2017

797-806

1 reference

A distinct interaction mode revealed by the crystal structure of the kinase p38α with the MAPK binding domain of the phosphatase MKP5

15 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Cross-talk between receptor tyrosine kinase and tumor necrosis factor-α signaling networks regulates apoptosis but not proliferation

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Phosphorylation of the kinase interaction motif in mitogen-activated protein (MAP) kinase phosphatase-4 mediates cross-talk between protein kinase A and MAP kinase signaling pathways

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Intersection of FOXO- and RUNX1-mediated gene expression programs in single breast epithelial cells during morphogenesis and tumor progression

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Feedback regulation of EGFR signalling: decision making by early and delayed loops.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Systematic quantification of negative feedback mechanisms in the extracellular signal-regulated kinase (ERK) signaling network.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Ligand-specific c-Fos expression emerges from the spatiotemporal control of ErbB network dynamics

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

In-gel protein phosphatase assay using fluorogenic substrates

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Cracking the phosphatase code: docking interactions determine substrate specificity

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Serine/threonine phosphatases: mechanism through structure

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

From promiscuity to precision: protein phosphatases get a makeover

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Input-output behavior of ErbB signaling pathways as revealed by a mass action model trained against dynamic data

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

A systems biological approach suggests that transcriptional feedback regulation by dual-specificity phosphatase 6 shapes extracellular signal-related kinase activity in RAS-transformed fibroblasts

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Cytokine-induced signaling networks prioritize dynamic range over signal strength

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

MKP-1 inhibits high NaCl-induced activation of p38 but does not inhibit the activation of TonEBP/OREBP: opposite roles of p38alpha and p38delta

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Dual-specificity MAP kinase phosphatases (MKPs) and cancer

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Integrative genomic approaches identify IKBKE as a breast cancer oncogene

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

A brief introduction to the protein phosphatase families

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Protein tyrosine phosphatases: from genes, to function, to disease

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

MAPK-specific tyrosine phosphatases: new targets for drug discovery?

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

The response of human epithelial cells to TNF involves an inducible autocrine cascade

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

A systems model of signaling identifies a molecular basis set for cytokine-induced apoptosis.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Spatially separate docking sites on ERK2 regulate distinct signaling events in vivo

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Structure and regulation of MAPK phosphatases

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

A functional variant of lymphoid tyrosine phosphatase is associated with type I diabetes

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

MAP kinase phosphatase as a locus of flexibility in a mitogen-activated protein kinase signaling network

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Amplification of PPM1D in human tumors abrogates p53 tumor-suppressor activity

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Reversible oxidation and inactivation of protein tyrosine phosphatases in vivo

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Mutations in PTPN11, encoding the protein tyrosine phosphatase SHP-2, cause Noonan syndrome

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

p38 mitogen-activated protein kinase-dependent activation of protein phosphatases 1 and 2A inhibits MEK1 and MEK2 activity and collagenase 1 (MMP-1) gene expression

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Extracellular signals and scores of phosphatases: all roads lead to MAP kinase

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Printing proteins as microarrays for high-throughput function determination

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Reduced MAP kinase phosphatase-1 degradation after p42/p44MAPK-dependent phosphorylation

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Crosstalk between cAMP-dependent kinase and MAP kinase through a protein tyrosine phosphatase

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Protein phosphatase 2Calpha inhibits the human stress-responsive p38 and JNK MAPK pathways

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Reversible inactivation of protein-tyrosine phosphatase 1B in A431 cells stimulated with epidermal growth factor

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Gene expression profiles in normal and cancer cells

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

The growth factor-inducible immediate-early gene 3CH134 encodes a protein-tyrosine-phosphatase

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Inactivation of p42 MAP kinase by protein phosphatase 2A and a protein tyrosine phosphatase, but not CL100, in various cell lines.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Pro-inflammatory cytokines and environmental stress cause p38 mitogen-activated protein kinase activation by dual phosphorylation on tyrosine and threonine

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Statistical analysis of radioimmunoassay data

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay.

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Permeabilizing cells: some methods and applications for the study of intracellular processes

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

A malachite green colorimetric assay for protein phosphatase activity

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3591670

Mathematical models of protein kinase signal transduction.

20 June 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

On the cross-regulation of protein tyrosine phosphatases and receptor tyrosine kinases in intracellular signaling.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

A continuous spectrophotometric and fluorimetric assay for protein tyrosine phosphatase using phosphotyrosine-containing peptides

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

An in-gel assay for protein tyrosine phosphatase activity: detection of widespread distribution in cells and tissues

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

p38alpha is active in vitro and in vivo when monophosphorylated at threonine 180.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

The activity of the extracellular signal-regulated kinase 2 is regulated by differential phosphorylation in the activation loop

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

A module of negative feedback regulators defines growth factor signaling

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

Strategies for protein coexpression in Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

Nuclear localization of Cdc25 is regulated by DNA damage and a 14-3-3 protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

Screening combinatorial libraries by mass spectrometry. 2. Identification of optimal substrates of protein tyrosine phosphatase SHP-1.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

A brake becomes an accelerator: PTP1B--a new therapeutic target for breast cancer.

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

Integration of MAP kinase signal transduction pathways at the serum response element

21 January 2018

1 reference

https://api.crossref.org/works/10.1074%2FMCP.O112.024059

Protein Phosphatases: Properties and Role in Cellular Regulation

21 January 2018

1 reference

12 December 2020

A nonradioactive fluorescent gel-shift assay for the analysis of protein phosphatase and kinase activities toward protein-specific peptide substrates

1 reference

12 December 2020

The dual specificity mitogen-activated protein kinase phosphatase-1 and -2 are induced by the p42/p44MAPK cascade

1 reference

12 December 2020

Mechanism of inhibition of protein-tyrosine phosphatases by vanadate and pervanadate

1 reference

12 December 2020

Protein phosphatase-1 and protein phosphatase-2A from rabbit skeletal muscle

1 reference

12 December 2020

A high-throughput quantitative multiplex kinase assay for monitoring information flow in signaling networks: application to sepsis-apoptosis

1 reference

12 December 2020

Calyculin A and okadaic acid: inhibitors of protein phosphatase activity

1 reference

12 December 2020

Phosphatase assay for multi-phosphorylated substrates using phosphatase specific-motif antibody

1 reference

12 December 2020

Fluoride at mitogenic doses induces a sustained activation of p44mapk, but not p42mapk, in human TE85 osteosarcoma cells

1 reference

12 December 2020

Role of c-Jun N-terminal kinase in apoptosis

1 reference

12 December 2020

A compendium of signals and responses triggered by prodeath and prosurvival cytokines

1 reference

12 December 2020

Limit of detection (LQD)/limit of quantitation (LOQ): comparison of the empirical and the statistical methods exemplified with GC-MS assays of abused drugs

1 reference

12 December 2020

A method for the rapid determination of alkaline phosphates with five cubic millimeters of serum

1 reference

12 December 2020

Development of an assay method for activities of serine/threonine protein phosphatase type 2B (calcineurin) in crude extracts

1 reference

12 December 2020

Identifiers

DOI

10.1074/MCP.O112.024059

1 reference

15 August 2017

1 reference

15 August 2017

1 reference

PMCID

3591670