Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. (Q30356050)

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English	Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.	scientific article

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scholarly article

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20 March 2020

Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins (English)

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20 March 2020

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ribonucleoprotein complex

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based on heuristic

inferred from title

Parthasarathy Seshacharyulu

12

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author name string

Rolen M Quadros

1

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Hiromi Miura

2

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Donald W Harms

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Hisako Akatsuka

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Takehito Sato

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Tomomi Aida

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Ronald Redder

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Guy P Richardson

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Yutaka Inagaki

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Daisuke Sakai

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Shannon M Buckley

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Surinder K Batra

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Sarah A Zeiner

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Ashley M Jacobi

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Yayoi Izu

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Wallace B Thoreson

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Lisa D Urness

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Suzanne L Mansour

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Masato Ohtsuka

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Channabasavaiah B Gurumurthy

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publication date

17 May 2017

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18

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92

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1

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20 March 2020

https://scigraph.springernature.com/pub.10.1186/s13059-017-1220-4

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Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes

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Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein.

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Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

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Rodent models in neuroscience research: is it a rat race?

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3 June 2018

A RATional choice for translational research?

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3 June 2018

CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice

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Regulation of Single-Strand Annealing and its Role in Genome Maintenance

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3 June 2018

CRISPR: a versatile tool for both forward and reverse genetics research.

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3 June 2018

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High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing.

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3 June 2018

CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells

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3 June 2018

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

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A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research

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3 June 2018

Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new

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Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases

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3 June 2018

Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system

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3 June 2018

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

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3 June 2018

GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos

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3 June 2018

A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes

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3 June 2018

Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium

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3 June 2018

Challenges to increasing targeting efficiency in genome engineering

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MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.

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3 June 2018

Expanding the power of recombinase-based labeling to uncover cellular diversity.

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Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes.

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Microhomology-Mediated End Joining: A Back-up Survival Mechanism or Dedicated Pathway?

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3 June 2018

Sequence determinants of improved CRISPR sgRNA design

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3 June 2018

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3 June 2018

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1 reference

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3 June 2018

Genome engineering using the CRISPR-Cas9 system

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3 June 2018

One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering

1 reference

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3 June 2018

RNA-guided human genome engineering via Cas9

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3 June 2018

Multiplex genome engineering using CRISPR/Cas systems

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3 June 2018

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3 June 2018

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Age-related hearing loss in C57BL/6J mice has both frequency-specific and non-frequency-specific components that produce a hyperacusis-like exaggeration of the acoustic startle reflex

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3 June 2018

A Mouse for All Reasons

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3 June 2018

Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction.

1 reference

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3 June 2018

Conservative homologous recombination preferentially repairs DNA double-strand breaks in the S phase of the cell cycle in human cells

1 reference

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3 June 2018

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1 reference

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3 June 2018

Conditional gene targeting

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21 January 2018

Generating rats with conditional alleles using CRISPR/Cas9.

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21 January 2018

Single-Step Generation of Conditional Knockout Mouse Embryonic Stem Cells.

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21 January 2018

A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival.

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21 January 2018

Generation of eGFP and Cre knockin rats by CRISPR/Cas9.

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21 January 2018

CRISPR mouse model boom, rat model renaissance.

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21 January 2018

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10.1186/S13059-017-1220-4

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