Production in two-liter beverage bottles of proteins for NMR structure determination labeled with either 15N- or 13C-15N. (Q30342327)

20 June 2017

Production in two-liter beverage bottles of proteins for NMR structure determination labeled with either 15N- or 13C-15N. (English)

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20 June 2017

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8

John L Markley

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20 June 2017

9

Brian G Fox

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20 June 2017

Qin Zhao

1

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20 June 2017

Ronnie Frederick

2

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20 June 2017

Kory Seder

3

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20 June 2017

Sandy Thao

4

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20 June 2017

Hassan Sreenath

5

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20 June 2017

Francis Peterson

6

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20 June 2017

Brian F Volkman

7

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20 June 2017

1 January 2004

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Journal of Structural and Functional Genomics

20 June 2017

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20 June 2017

5

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20 June 2017

1-2

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20 June 2017

87-93

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Application of fed-batch fermentation to the preparation of isotopically labeled or selenomethionyl-labeled proteins

20 June 2017

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12 December 2020

Reduced background expression and improved plasmid stability with pET vectors in BL21 (DE3)

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12 December 2020

A novel isotope labeling protocol for bacterially expressed proteins

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12 December 2020

Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

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12 December 2020

Production of selenomethionine-labeled proteins in two-liter plastic bottles for structure determination

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12 December 2020

A method for efficient isotopic labeling of recombinant proteins

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12 December 2020

An integrated approach to structural genomics.

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12 December 2020

An efficient NMR approach for obtaining sequence-specific resonance assignments of larger proteins based on multiple isotopic labeling.

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12 December 2020

Parallel cloning, expression, purification and crystallization of human proteins for structural genomics

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12 December 2020

Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins

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12 December 2020

Controlling basal expression in an inducible T7 expression system by blocking the target T7 promoter with lac repressor

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12 December 2020

Application of NMR in structural proteomics: screening for proteins amenable to structural analysis

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12 December 2020

A less laborious approach to the high-throughput production of recombinant proteins in Escherichia coli using 2-liter plastic bottles

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12 December 2020

Improved synthetic methods for the selective deuteration of aromatic amino acids: applications of selective protonation towards the identification of protein folding intermediates through nuclear magnetic resonance.

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12 December 2020

An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli

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12 December 2020

Role for NMR in structural genomics

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12 December 2020

High-throughput proteomics: a flexible and efficient pipeline for protein production

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12 December 2020

NMR docking of a substrate into the X-ray structure of staphylococcal nuclease

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12 December 2020

New approaches to structure determination by NMR spectroscopy

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12 December 2020

Biosynthetic incorporation of 15N and 13C for assignment and interpretation of nuclear magnetic resonance spectra of proteins

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12 December 2020

10.1023/B:JSFG.0000029205.65813.42

Identifiers

DOI

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Dimensions Publication ID

20 June 2017

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