How enzymes adapt: lessons from directed evolution (Q28199605)

Trends in Biochemical Sciences

Europe PubMed Central

title

How enzymes adapt: lessons from directed evolution (English)

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A Gershenson

4

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author name string

F H Arnold

series ordinal

1

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P L Wintrode

series ordinal

2

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K Miyazaki

series ordinal

3

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language of work or name

English

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publication date

February 2001

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published in

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volume

26

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issue

2

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page(s)

100-6

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cites work

The stability of proteins in extreme environments

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

The crystal structure of citrate synthase from the hyperthermophilic archaeon pyrococcus furiosus at 1.9 A resolution,

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Structural adaptations of the cold-active citrate synthase from an Antarctic bacterium

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Finding and using hyperthermophilic enzymes.

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Rigidity of Thermophilic Enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Molecular evolution by staggered extension process (StEP) in vitro recombination

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Directed evolution study of temperature adaptation in a psychrophilic enzyme

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Improving the catalytic activity of a thermophilic enzyme at low temperatures

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Directed evolution of a thermostable esterase.

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Tryptophan phosphorescence study of enzyme flexibility and unfolding in laboratory-evolved thermostable esterases

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Directed evolution converts subtilisin E into a functional equivalent of thermitase

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

A cold-adapted protease engineered by experimental evolution system

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Improving low-temperature catalysis in the hyperthermostable Pyrococcus furiosus beta-glucosidase CelB by directed evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

A structural view of evolutionary divergence

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Stability and Activity of Mesophilic Subtilisin E and Its Thermophilic Homolog: Insights from Molecular Dynamics Simulations

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Enzymes under extremes of physical conditions

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Extremely thermostable D-glyceraldehyde-3-phosphate dehydrogenase from the eubacterium Thermotoga maritima

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Amino acid substitutions enhancing thermostability of Bacillus polymyxa beta-glucosidase A

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Further improvement of the thermal stability of a partially stabilized Bacillus subtilis 3-isopropylmalate dehydrogenase variant by random and site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Engineering an enzyme to resist boiling

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

Structural and mutagenesis studies of leishmania triosephosphate isomerase: a point mutation can convert a mesophilic enzyme into a superstable enzyme without losing catalytic power

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021

The hyperthermostable indoleglycerol phosphate synthase from Thermotoga maritima is destabilized by mutational disruption of two solvent-exposed salt bridges.

1 reference

https://api.crossref.org/works/10.1016%2FS0968-0004%2800%2901755-2

7 January 2021