Single copy shRNA configuration for ubiquitous gene knockdown in mice (Q24797255)

1

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Birgit Küter-Luks

2

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Heidrun Kern

3

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Sandra Streu

4

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Leona Plum

5

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Jan Mauer

6

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Ralf Kühn

7

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Frieder Schwenk

Cre-lox-regulated conditional RNA interference from transgenes

9

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language of work or name

English

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publication date

2005

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published in

Nucleic Acids Research

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volume

33

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issue

7

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page(s)

e67

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cites work

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

A system for stable expression of short interfering RNAs in mammalian cells

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Generalized lacZ expression with the ROSA26 Cre reporter strain

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Leptin and the regulation of body weight in mammals

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Efficiency of RNA interference in the mouse hematopoietic system varies between cell types and developmental stages

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Establishment of conditional vectors for hairpin siRNA knockdowns

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Rapid generation of inducible mouse mutants

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Small interfering RNA and gene silencing in transgenic mice and rats

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Real-time quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

A genetic screen identifies novel non-compatible loxP sites.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

'Green mice' as a source of ubiquitous green cells.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Improved properties of FLP recombinase evolved by cycling mutagenesis.

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype.

30 May 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Germline transmission of RNAi in mice

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Efficient FLP recombination in mouse ES cells and oocytes.

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1079974

Development of 1-cell embryos from different strains of mice in CZB medium

28 November 2018

1 reference

12 December 2020

DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs

1 reference

12 December 2020

Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange

1 reference

12 December 2020

Selection-marker-free modification of the murine beta-casein gene using a lox2272 [correction of lox2722] site

1 reference

12 December 2020

Expanding small RNA interference

1 reference

12 December 2020

Efficient delivery of siRNA for inhibition of gene expression in postnatal mice

1 reference

12 December 2020

Site-specific cassette exchange and germline transmission with mouse ES cells expressing phiC31 integrase

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/GNI065

1 reference

3679232

3679232

1 reference

1 reference

1 reference