Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex (Q21284398)

18 August 2022

Ji Hun Kim

1

Ji Hun

Kim

1 reference

18 August 2022

Tsz M Chang

2

Tsz M

Chang

1 reference

18 August 2022

Alison N Graham

3

Alison N

Graham

1 reference

18 August 2022

K H A Choo

4

K H

Andy Choo

1 reference

18 August 2022

Damien F Hudson

6

Damien F

Hudson

1 reference

describes a project that uses

18 August 2022

language of work or name

English

0 references

publication date

2010

0 references

published in

BMC Biochemistry

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volume

11

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issue

1

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page(s)

50

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ImageJ

1 reference

Europe PubMed Central

11 June 2022

https://www.ebi.ac.uk/europepmc/webservices/rest/PMC3022668/fullTextXML

based on heuristic

inferred from PubMed Central ID database lookup

cites work

Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein research

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

The use of mRNA display to select high-affinity protein-binding peptides

19 March 2017

1 reference

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Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

19 March 2017

1 reference

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ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure.

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Genome-wide mapping of in vivo protein-DNA interactions

19 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Condensin: Architect of mitotic chromosomes

7 April 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Proteomic analysis of cartilage proteins

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Condensins: organizing and segregating the genome

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Distinct functions of condensin I and II in mitotic chromosome assembly

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells.

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Condensin is required for nonhistone protein assembly and structural integrity of vertebrate mitotic chromosomes

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Homologous recombination, but not DNA repair, is reduced in vertebrate cells deficient in RAD52.

12 September 2017

1 reference

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ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation

12 September 2017

1 reference

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The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Molecular and genetic analysis of condensin function in vertebrate cells

12 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Condensin I stabilizes chromosomes mechanically through a dynamic interaction in live cells.

27 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Comparison of affinity tags for protein purification

29 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification.

29 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3022668

Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins

29 November 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21194474

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/21194474

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1186/1471-2091-11-50

0 references