Construction of Normalized Full-length cDNA Library and EST Analysis from Cestrum nocturnum L. (Q101107022)
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scientific article published in 2016
| Language | Label | Description | Also known as |
|---|---|---|---|
| English | Construction of Normalized Full-length cDNA Library and EST Analysis from Cestrum nocturnum L. |
scientific article published in 2016 |
Statements
夜香树均一化全长cDNA文库的构建及EST分析 (Chinese)
Construction of Normalized Full-length cDNA Library and EST Analysis from Cestrum nocturnum L. (English)
Cestrum nocturnum
1 reference
郑鸿昌
刘涛
吕恃衡
陈建军
何水林
潘东明
陈桂信
ZHENG Hong-chang
LIU Tao
L(U) Shi-heng
CHEN Jian-jun
HE Shui-lin
PAN Dong-ming
CHEN Gui-xin
为构建高质量的夜香树(Cestrum nocturnum)均一化全长cDNA文库,以其花朵为材料,采用DSN均一化技术与改进的SMART技术相结合,将连接产物进行脱盐浓缩后电转化进行构建.结果表明,未脱盐连接产物的转化效率为1 μL连接产物有7000个菌落,理论重组率为96％;脱盐后,提升为4.1×106个菌落,理论重组率为98％;蓝斑也有插入片段,实际重组率应为100％;插入片段大小平均为1.6… (Chinese)
0 references