Real-time quantitative PCR for the design of lentiviral vector analytical assays (Q36288916)

13 August 2017

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title

Real-time quantitative PCR for the design of lentiviral vector analytical assays (English)

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13 August 2017

Delenda C

1

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PubMed ID

16231054

13 August 2017

Gaillard C

series ordinal

2

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13 August 2017

1 October 2005

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13 August 2017

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13 August 2017

12 Suppl 1

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13 August 2017

S36-50

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https://scigraph.springernature.com/pub.10.1038/sj.gt.3302614

13 August 2017

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Regulating gene expression using self-inactivating lentiviral vectors containing the mifepristone-inducible system

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Five recombinant simian immunodeficiency virus pseudotypes lead to exclusive transduction of retinal pigmented epithelium in rat.

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Long-term, targeted genetic modification of the aqueous humor outflow tract coupled with noninvasive imaging of gene expression in vivo

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21 January 2018

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An RT-PCR assay for the enzyme activity of reverse transcriptase capable of detecting single virions

21 January 2018

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Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement

21 January 2018

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Development of an assay that detects transcriptionally competent human immunodeficiency virus type one particles

21 January 2018

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Comparable sensitivities for detection of HIV-1 reverse transcriptase (RT) and other polymerases by RT assays requiring no radioisotopic materials

21 January 2018

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Quantitative detection of RT activity by PERT assay: feasibility and limits to a standardized screening assay for human vaccines

21 January 2018

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Development and characterization of a minimal inducible packaging cell line for simian immunodeficiency virus-based lentiviral vectors

21 January 2018

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New methods to titrate EIAV-based lentiviral vectors

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A new-generation stable inducible packaging cell line for lentiviral vectors

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A simple and reliable method for screening retroviral producer clones without selectable markers

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Detection of the human immunodeficiency virus genome with a biotinylated DNA probe generated by polymerase chain reaction

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Investigation of recombinant human insulin-like growth factor type I in thymus regeneration in the acute stage of experimental FIV infection in juvenile cats.

21 January 2018

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Comparative analysis of HIV-1 viral load assays on subtype quantification: Bayer Versant HIV-1 RNA 3.0 versus Roche Amplicor HIV-1 Monitor version 1.5.

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Sensitive detection of genetic variants of HIV-1 and HCV with an HIV-1/HCV assay based on transcription-mediated amplification

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Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods

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Gene transduction efficiency in cells of different species by HIV and EIAV vectors

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Real-Time Quantitative Reverse Transcriptase-Polymerase Chain Reaction as a Method for Determining Lentiviral Vector Titers and Measuring Transgene Expression

21 January 2018

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One step screening of retroviral producer clones by real time quantitative PCR.

21 January 2018

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A quantitative assay for HIV DNA integration in vivo

21 January 2018

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A lentiviral vector encoding the human Wiskott-Aldrich syndrome protein corrects immune and cytoskeletal defects in WASP knockout mice.

21 January 2018

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Stable RNA interference (RNAi) as an option for anti-bcr-abl therapy.

21 January 2018

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The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events

21 January 2018

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Evaluation of plasmid DNA removal from lentiviral vectors by benzonase treatment.

21 January 2018

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Unintegrated lentivirus DNA persistence and accessibility to expression in nondividing cells: analysis with class I integrase mutants

21 January 2018

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https://api.crossref.org/works/10.1038%2FSJ.GT.3302614

Impact of the central polypurine tract on the kinetics of human immunodeficiency virus type 1 vector transduction

21 January 2018

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https://api.crossref.org/works/10.1038%2FSJ.GT.3302614

In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector

21 January 2018

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Characterization and detection of artificial replication-competent lentivirus of altered host range

21 January 2018

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Certification assays for HIV-1-based vectors: frequent passage of gag sequences without evidence of replication-competent viruses

21 January 2018

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Long-term transgene expression by administration of a lentivirus-based vector to the fetal circulation of immuno-competent mice.

21 January 2018

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Development of a novel trans-lentiviral vector that affords predictable safety

21 January 2018

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Modified human immunodeficiency virus-based lentiviral vectors display decreased sensitivity to trans-dominant Rev.

21 January 2018

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https://api.crossref.org/works/10.1038%2FSJ.GT.3302614

Development of a sensitive assay for detection of replication-competent recombinant lentivirus in large-scale HIV-based vector preparations

21 January 2018

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Preclinical development strategies for novel gene therapeutic products

21 January 2018

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Gene transfer vector biodistribution: pivotal safety studies in clinical gene therapy development

21 January 2018

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Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors

21 January 2018

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Pseudotyped human lentiviral vector-mediated gene transfer to airway epithelia in vivo.

21 January 2018

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https://api.crossref.org/works/10.1038%2FSJ.GT.3302614

Role of hepatocyte direct hyperplasia in lentivirus-mediated liver transduction in vivo

21 January 2018

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https://api.crossref.org/works/10.1038%2FSJ.GT.3302614

Organ distribution of gene expression after intravenous infusion of targeted and untargeted lentiviral vectors.

21 January 2018

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Lentiviral vectors containing the human immunodeficiency virus type-1 central polypurine tract can efficiently transduce nondividing hepatocytes and antigen-presenting cells in vivo

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Novel integrase-defective lentiviral episomal vectors for gene transfer

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Biodistribution and toxicity studies of VSVG-pseudotyped lentiviral vector after intravenous administration in mice with the observation of in vivo transduction of bone marrow

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1 reference

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Efficient transduction of liver and muscle after in utero injection of lentiviral vectors with different pseudotypes

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Multiply attenuated, self-inactivating lentiviral vectors efficiently deliver and express genes for extended periods of time in adult rat cardiomyocytes in vivo.

21 January 2018

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Phenotype correction of Fanconi anemia group A hematopoietic stem cells using lentiviral vector

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Efficient marking of murine long-term repopulating stem cells targeting unseparated marrow cells at low lentiviral vector particle concentration

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Efficient gene transfer into rhesus repopulating hematopoietic stem cells using a simian immunodeficiency virus-based lentiviral vector system

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Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through intravenous injection

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Gene therapy for a mucopolysaccharidosis type I murine model with lentiviral-IDUA vector.

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An inherited limb deformity created by insertional mutagenesis in a transgenic mouse

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Legless, a Novel Mutation Found in PHT1-1 Transgenic Mice

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Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences

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Clinical Evaluation of Branched DNA Signal Amplification for Quantifying HIV Type 1 in Human Plasma

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Detection of intracellular interleukin-8 in human mast cells: flow cytometry as a guide for immunoelectron microscopy

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Intracellular delivery strategies for antisense phosphorodiamidate morpholino oligomers

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Assay for type C virus in mouse sera based on particulate reverse transcriptase activity

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A new method for measuring reverse transcriptase activity by ELISA

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Single-tube fluorescent product-enhanced reverse transcriptase assay with Ampliwax (STF-PERT) for retrovirus quantitation

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Plasma viremia in macaques infected with simian immunodeficiency virus: plasma viral load early in infection predicts survival.

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Quantitative determination of lentiviral vector particle numbers by real-time PCR

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Lentivirus-mediated transduction of islet grafts with interleukin 4 results in sustained gene expression and protection from insulitis

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High-titer human immunodeficiency virus type 1-based vector systems for gene delivery into nondividing cells.

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Development of a self-inactivating lentivirus vector.

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Identifiers

DOI

10.1038/SJ.GT.3302614

1 reference

PubMed ID

16231054

Dimensions Publication ID

13 August 2017

0 references

1 reference

PubMed ID

16231054