A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). (Q36205906)

12 August 2017

A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). (English)

1 reference

12 August 2017

R B Vallee

1

1 reference

PMCID

2112074

12 August 2017

language of work or name

English

0 references

publication date

1 February 1982

1 reference

12 August 2017

Journal of Cell Biology

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12 August 2017

92

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12 August 2017

2

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12 August 2017

435-442

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Protein measurement with the Folin phenol reagent

12 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

High molecular weight microtubule-associated proteins are preferentially associated with dendritic microtubules in brain

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

A protein kinase bound to the projection portion of MAP 2 (microtubule-associated protein 2)

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Structure and phosphorylation of microtubule-associated protein 2 (MAP 2)

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Taxol stabilizes microtubules in mouse fibroblast cells

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Microtubule Assembly in the Absence of Added Nucleotides

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Dynamics of mitotic spindle organization and function

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Tubulin requires tau for growth onto microtubule initiating sites

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Polycation-induced assembly of purified tubulin

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Inhibition of tubulin assembly by RNA and other polyanions: evidence for a required protein

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

A protein factor essential for microtubule assembly

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

In vitro polymerization of microtubules from HeLa cells

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

The periodic association of MAP2 with brain microtubules in vitro

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Self-assembly of microtubules in extracts of cultured HeLa cells and the identification of HeLa microtubule-associated proteins

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Addition of colchicine-tubulin complex to microtubule ends: The mechanism of substoichiometric colchicine poisoning

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

The equilibrium assembly of microtubules in vitro

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Ionic and nucleotide requirements for microtubule polymerization in vitro

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Conservation of microtubule associated proteins. Isolation and characterization of tau and the high molecular weight microtubule associated protein from chicken brain and from mouse fibroblasts and comparison to the corresponding mammalian brain pro

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Physical and chemical properties of purified tau factor and the role of tau in microtubule assembly

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Arrangement of high molecular weight associated proteins on purified mammalian brain microtubules

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Nucleated assembly of microtubules in porcine brain extracts.

1 October 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

A simplified method for the quantitative assay of small amounts of protein in biologic material

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules.

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Comparison of the sedimentation properties of microtubule protein oligomers prepared by two different procedures

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Separation and characterization of microtubule proteins from calf brain

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules.

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Removal of the projections from cytoplasmic microtubules in vitro by digestion with trypsin

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

In vitro reconstitution of calf brain microtubules: effects of macromolecules.

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Fractionation of brain microtubule-associated proteins. Isolation of two different proteins which stimulate tubulin polymerization in vitro

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

A high molecular weight phosphoprotein in cultured fibroblasts that associates with polymerized tubulin

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Role of tubulin-associated proteins in microtubule nucleation and elongation

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Promotion of microtubule assembly in vitro by taxol

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

Microtubule-associated proteins and the stimulation of tubulin assembly in vitro

29 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2112074

The non-tubulin component of microtubule protein oligomers. Effect on self-association and hydrodynamic properties

22 September 2018

1 reference

12 December 2020

Identification with cellular microtubules of one of the co-assembling microtubule-associated proteins

1 reference

12 December 2020

Test of four possible mechanisms for the temporal control of spindle and cytoplasmic microtubule assembly in HeLa cells

1 reference

12 December 2020

Purification and reconstitution of HeLa cell microtubules

1 reference

12 December 2020

Microtubule-associated proteins from cultured HeLa cells. Analysis of molecular properties and effects on microtubule polymerization

1 reference

12 December 2020

A microtubule-associated protein specific to differentiated neuroblastoma cells

1 reference

12 December 2020

Molecular analysis of cytoplasmic microtubules in situ: Identification of both widespread and specific proteins

1 reference

12 December 2020

Identifiers

DOI

10.1083/JCB.92.2.435

1 reference

12 August 2017

1 reference

12 August 2017

1 reference

PMCID

2112074