José María Requena (Q58252822)

Leishmania braziliensis MHOM/BR/75/M2904

29273719

1 reference

30540030

Leishmania donovani HU3

1 reference

Leishmania major Friedlin

30992521

1 reference

Autonomous University of Madrid

34573340

employer

2 references

12 January 2019

https://portalcientifico.uam.es/es/ipublic/researcher/259327

educated at

Autonomous University of Madrid

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academic degree

Doctor of Philosophy

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licensed

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Doktor of Biology Sciences

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0 references

1 reference

parasitology

1 reference

virology

1 reference

A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi

notable work

1 reference

Complete nucleotide sequence of the hsp70 gene of T. cruzi

2831499

1 reference

Molecular cloning of a Drosophila potential Z-DNA forming sequence hybridizing in situ to a developmentally regulated subdivision of the polytene chromosomes

2644623

1 reference

The heat shock protein hsp70 binds in vivo to subregions 2-48BC and 3-58D of the polytene chromosomes of Drosophila hydei

2473437

1 reference

Sequence and expression of the Drosophila phenylalanine hydroxylase mRNA.

2265568

1 reference

A potential Z-DNA-forming sequence is located between two transcription units alternatively expressed during development of Drosophila hydei

2121612

1 reference

DNA binding properties and antileukemic (L1210) activity of a Pt-pentamidine complex

1840694

1 reference

The mRNA coding for the nucleosomal protein H2A ofLeishmaniais polyadenylated and has stem-loops at the 3′ end

2009576

1 reference

Characterization of a highly repeated interspersed DNA sequence of Trypanosoma cruzi: its potential use in diagnosis and strain classification

1679534

1 reference

Molecular characterization of a Leishmania donovani infantum antigen identified as histone H2A

1574085

1 reference

Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase

1555581

1 reference

Bacteriophage lambda: long-term stored stocks ready for lysis

1501640

1 reference

Mapping of antigenic determinants of the T. cruzi hsp70 in chagasic and healthy individuals

8434416

1 reference

Binding of Pt-pentamidine to nucleosomal DNA. Studies of the antiproliferative activity of the drug against human cancer cells

8366861

1 reference

Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania.

8221967

1 reference

Isolation of Trypanosoma cruzi specific nuclear repeated DNA sequences

8408038

1 reference

Cytoplasmic-nuclear translocation of the Hsp70 protein during environmental stress in Trypanosoma cruzi

7670522

1 reference

The anti Z-DNA reactivity of Z-DNA forming sequences is affected by platinum antitumor drugs.

8250874

1 reference

The Leishmania infantum histone H3 possesses an extremely divergent N-terminal domain

8227037

1 reference

During active viscerocutaneous leishmaniasis the anti-P2 humoral response is specifically triggered by the parasite P proteins

7918653

1 reference

Assignment of genes to Leishmania infantum chromosomes: karyotype and ploidy

7743663

1 reference

Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi genome project

7781987

1 reference

Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis

7477112

1 reference

Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis

8847086

1 reference

During canine viscero-cutaneous leishmaniasis the anti-Hsp70 antibodies are specifically elicited by the parasite protein

8867853

1 reference

Genomic repetitive DNA elements of Trypanosoma cruzi

8728991

1 reference

Specific serodiagnosis of human leishmaniasis with recombinant Leishmania P2 acidic ribosomal proteins.

15275193

1 reference

Organization, transcription and regulation of the Leishmania infantum histone H3 genes

8807201

1 reference

Mapping of the linear antigenic determinants of the Leishmania infantum hsp70 recognized by leishmaniasis sera

8836124

1 reference

Presence of antibodies in the aqueous humour and cerebrospinal fluid during Leishmania infections in dogs. Pathological features at the central nervous system

8905399

1 reference

Characterization of the antigenic determinants of the Leishmania infantum histone H3 recognized by antibodies elicited during canine visceral leishmaniasis

9226692

1 reference

During canine leishmaniasis a protein belonging to the 83-kDa heat-shock protein family elicits a strong humoral response

8973612

1 reference

Analysis of post-transcriptional regulation operating on transcription products of the tandemly linked Leishmania infantum hsp70 genes

8971277

1 reference

Cloning of genes and expression and antigenicity analysis of the Leishmania infantum KMP-11 protein

9020174

1 reference

A region containing repeated elements is associated with transcriptional termination of Leishmania infantum ribosomal RNA genes

9024210

1 reference

Mapping of the antigenic determinants of the Leishmania infantum gp63 protein recognized by antibodies elicited during canine visceral leishmaniasis.

9041525

1 reference

Efficient 5′-End Labeling of Oligonucleotides Containing Self-Complementary Sequences

9172421

1 reference

Towards the physical map of the Trypanosoma cruzi nuclear genome: construction of YAC and BAC libraries of the reference clone T. cruzi CL-Brener

9343687

1 reference

Genes and Chromosomes of Leishmania infantum

9566217

1 reference

Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi

9566218

1 reference

Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes

9488867

1 reference

Multicomponent Chimeric Antigen for Serodiagnosis of Canine Visceral Leishmaniasis

9476792

1 reference

Characterization of the Immunostimulatory Properties ofLeishmania infantumHSP70 by Fusion to theEscherichia coliMaltose-Binding Protein in Normal andnu/nuBALB/c Mice

9431920

1 reference

Cloning, sequencing, and expression of the PSA genes from Leishmania infantum

9423878

1 reference

Polymerase Chain Reaction Procedure to Detect Trypanosoma cruzi in Blood Samples from Chronic Chagasic Patients

9492309

1 reference

Analysis of the antigenic properties of the L. infantum Hsp70: design of synthetic peptides for specific serodiagnosis of human leishmaniasis

9572024

1 reference

Phage recovery by electroporation of naked DNA into host cells avoids the use of packaging extracts.

9840686

1 reference

Antigenicity of the Leishmania infantum histones H2B and H4 during canine viscerocutaneous leishmaniasis.

9918677

1 reference

Analysis of the humoral immune response against total and recombinant antigens of Leishmania infantum: correlation with disease progression in canine experimental leishmaniasis

9933463

1 reference

Immunostimulatory properties of the Leishmania infantum heat shock proteins HSP70 and HSP83

10077418

1 reference

Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control

10698315

1 reference

Evolutionarily conserved proteins as prominent immunogens during Leishmania infections

10657245

1 reference

Identification of a putative regulatory element in the 3'-untranslated region that controls expression of HSP70 in Leishmania infantum

10827430

1 reference

Genomic clustering of the Trypanosoma cruzi nonlong terminal L1Tc retrotransposon with defined interspersed repeated DNA elements

10989147

1 reference

Leishmania infantum: gene cloning of the GRP94 homologue, its expression as recombinant protein, and analysis of antigenicity

11001312

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The Leishmania infantum acidic ribosomal protein LiP2a induces a prominent humoral response in vivo and stimulates cell proliferation in vitro and interferon-gamma (IFN-gamma) production by murine splenocytes

11052869

1 reference

Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model

11091277

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More panantigens in Leishmania

11044559

1 reference

Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination

11228008

1 reference

Characterisation of a monoclonal antibody recognising specifically the HSP70 from Leishmania

11274727

1 reference

Antigenic properties of the Leishmania infantum GRP94 and mapping of linear B-cell epitopes

11728013

1 reference

The heat shock proteins, Hsp70 and Hsp83, of Leishmania infantum are mitogens for mouse B cells

11803053

1 reference

A Leishmania infantum multi-component antigenic protein mixed with live BCG confers protection to dogs experimentally infected with L. infantum

12653478

1 reference

Conserved nucleotides surrounding the trans-splicing acceptor site and the translation initiation codon in Leishmania genes

12628759

1 reference

Leishmania infantum possesses a complex family of histone H2A genes: structural characterization and analysis of expression

12810050

1 reference

Identification of a gene in Leishmania infantum encoding a protein that contains a SP-RING/MIZ zinc finger domain

12954010

1 reference

High-efficiency plating method for Leishmania infantum

22883986

1 reference

The Leishmania infantum acidic ribosomal protein P0 administered as a DNA vaccine confers protective immunity to Leishmania major infection in BALB/c mice

12946851

1 reference

Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani.

14573678

1 reference

Cell-cycle-dependent translation of histone mRNAs is the key control point for regulation of histone biosynthesis in Leishmania infantum

14622299

1 reference

The expression of HSP83 genes in Leishmania infantum is affected by temperature and by stage-differentiation and is regulated at the levels of mRNA stability and translation

14766017

1 reference

Recent advances in vaccines for leishmaniasis

15176985

1 reference

Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniosis

15335317

1 reference

Adjuvant guided polarization of the immune humoral response against a protective multicomponent antigenic protein (Q) from Leishmania infantum. A CpG + Q mix protects Balb/c mice from infection

15364433

1 reference

Genomic DNA macroarrays as a tool for analysis of gene expression in Leishmania

15541032

1 reference

The translational efficiencies of the two Leishmania infantum HSP70 mRNAs, differing in their 3'-untranslated regions, are affected by shifts in the temperature of growth through different mechanisms

15936759

1 reference

Immunohistological features of visceral leishmaniasis in BALB/c mice

16105831

1 reference

Genomic organization and expression of the HSP70 locus in New and Old World Leishmania species

16629702

1 reference

A postgenomic view of the heat shock proteins in kinetoplastids

17054823

1 reference

Pitfalls of the CAT reporter gene for analyzing translational regulation in Leishmania

17459115

1 reference

Immunogenicity of HSP-70, KMP-11 and PFR-2 leishmanial antigens in the experimental model of canine visceral leishmaniasis

17676404

1 reference

The Leishmania HSP20 is antigenic during natural infections, but, as DNA vaccine, it does not protect BALB/c mice against experimental L. amazonensis infection

18321614

1 reference

The SIDER2 elements, interspersed repeated sequences that populate the Leishmania genomes, constitute subfamilies showing chromosomal proximity relationship

18401455

1 reference

Effects of the disruption of the HSP70-II gene on the growth, morphology, and virulence of Leishmania infantum promastigotes.

18518959

1 reference

The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis

18645958

1 reference

Evidence of RNA editing in Leishmania braziliensis promastigotes

20180988

1 reference

Lights and shadows on gene organization and regulation of gene expression in Leishmania

21132328

1 reference

Maxicircle genome partial sequence of leishmania braziliensis: Assembling and comparison with other trypanosomatids

21622163

1 reference

DOI

10.11144/JAVERIANA.SC16-1.MGPS

Leishmania infantum HSP70-II null mutant as candidate vaccine against leishmaniasis: a preliminary evaluation

1 reference

Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization

21794145

1 reference

Immunity to visceral leishmaniasis

21871099

1 reference

Sequence analysis of the 3’-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification

22253634

1 reference

Indotecan (LMP400) and AM13-55: two novel indenoisoquinolines show potential for treating visceral leishmaniasis

22541251

1 reference

Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis

22850521

1 reference

The transcriptome of Leishmania major in the axenic promastigote stage: transcript annotation and relative expression levels by RNA-seq.

23209866

1 reference

Gimatecan and other camptothecin derivatives poison Leishmania DNA-topoisomerase IB leading to a strong leishmanicidal effect.

23557257

1 reference

Evolution and species discrimination according to the Leishmania heat-shock protein 20 gene

23466420

1 reference

Alpha tubulin genes from Leishmania braziliensis: genomic organization, gene structure and insights on their expression

23722022

1 reference

Identification of proteins interacting with HSP70 mRNAs in Leishmania braziliensis.

23829570

1 reference

Detection of Leishmania spp. based on the gene encoding HSP20

24060997

1 reference

Leishmania braziliensis replication protein A subunit 1: molecular modelling, protein expression and analysis of its affinity for both DNA and RNA.

25597712

1 reference

Molecular Chaperones of Leishmania: Central Players in Many Stress-Related and -Unrelated Physiological Processes

25498946

1 reference

Resequencing and assembly of seven complex loci to improve the Leishmania major (Friedlin strain) reference genome

26167482

1 reference

A putative Leishmania DNA polymerase theta protects the parasite against oxidative damage.

26857920

1 reference

Transcriptionally Driven DNA Replication Program of the Human Parasite Leishmania major

27131366

1 reference

Genomic cartography and proposal of nomenclature for the repeated, interspersed elements of the Leishmania major SIDER2 family and identification of SIDER2-containing transcripts.

27477279

1 reference

Dataset for distribution of SIDER2 elements in the Leishmania major genome and transcriptome.

28034676

1 reference

Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models

28127581

1 reference

Pre-clinical antigenicity studies of an innovative multivalent vaccine for human visceral leishmaniasis

28558043

1 reference

Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity.

29176865

1 reference

Resequencing of the Leishmania infantum (strain JPCM5) genome and de novo assembly into 36 contigs.

29258569

1 reference

Dipeptidyl peptidase 3, a novel protease from Leishmania braziliensis.

29273719

1 reference

Vaccine candidates against leishmania under current research.

29304092

1 reference

Genomic and transcriptomic alterations in Leishmania donovani lines experimentally resistant to antileishmanial drugs

29589966

1 reference

Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis

29689531

1 reference

Complete and de novo assembly of the Leishmania braziliensis (M2904) genome

29675401

1 reference

Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation

30540030

2 references

https://rdcu.be/bxaEi

Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major

30992521

2 references

31061406

Leishmania Mitochondrial Genomes: Maxicircle Structure and Heterogeneity of Minicircles

https://rdcu.be/bAv1b

1 reference

Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi

31561572

1 reference

Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice

32244527

1 reference

Draft Genome Sequence of the Trypanosoma cruzi B. M. López Strain (TcIa), Isolated from a Colombian Patient

32210040

1 reference

Trypanosoma cruzi Ikiakarora (TcIII) Draft Genome Sequence

32354964

1 reference

Isolation, characterization and analysis of the expression of the Leishmania ribosomal P0 protein genes

32616633

1 reference

Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3'-end of a poly(A)+ transcript.

8264730

1 reference

Leishmania infantum transfected with toxic plasmid induces protection in mice infected with wild type L. infantum or L. amazonensis

8076826

1 reference

The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations

32949849

1 reference

Identification of the interactomes associated with SCD6 and RBP42 proteins in Leishmania braziliensis

32887454

1 reference

Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice

33296709

1 reference

Proteins interacting with Leishmania major PUF1: A proteomic dataset.

33673117

1 reference

Variability of the Pr77 sequence of L1Tc retrotransposon among six T. cruzi strains belonging to different discrete typing units (DTUs)

34026959

1 reference

Gene annotation and transcriptome delineation on a de novo genome assembly for the reference Leishmania major friedlin strain

34273311

1 reference

Contributions to the evolution and taxonomic classification of medical importance genus of the Trypanosomatidae family based on the phylogenetic analysis of the heat shock protein 70 gene

34573340

1 reference

http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S2304-01062021000100025

Protective Efficacy in a Hamster Model of a Multivalent Vaccine for Human Visceral Leishmaniasis (MuLeVaClin) Consisting of the KMP11, LEISH-F3+, and LJL143 Antigens in Virosomes, Plus GLA-SE Adjuvant

1 reference

ARAMIS: From systematic errors of NGS long reads to accurate assemblies

34835379

1 reference

A dataset of proteins associated with Trypanosoma cruzi LYT1 mRNAs

34013348

1 reference

Live attenuated vaccines, a favorable strategy to provide long-term immunity against protozoan diseases

35242934

1 reference

The Astonishing Large Family of HSP40/DnaJ Proteins Existing in Leishmania

34896016

1 reference

Assembly of a Large Collection of Maxicircle Sequences and Their Usefulness for Leishmania Taxonomy and Strain Typing

35627127

1 reference

Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing

35741832

10.11144/JAVERIANA.SC23-2.COTM

1 reference

DOI

Immunization with Leishmania amazonensis subgenomic libraries protects BALB/c mice against the challenge

Spanish

1 reference

Cloning of 20 kDa heat shock protein of Leishmania amazonensis

15846906

ResearchGate publication ID

Spanish

1 reference

stated in

ResearchGate

259622701

https://www.researchgate.net/publication/259622701

Identification of regulatory proteins of gene expression in trypanosomatids

Spanish

1 reference

http://dx.doi.org/10.18257/raccefyn.671

Kinetoplastid Genomics and Beyond

1 reference

https://www.mdpi.com/books/pdfview/book/4005

Omics approaches for understanding gene expression in Leishmania: clues for tackling leishmaniasis

1 reference

https://www.caister.com/openaccess/pdf/9781910190838-05.pdf

Virulence factors and immune evasion in Leishmania

http://www.eurekaselect.com/ebook_volume/2201

1 reference

official website

Stress Response in Microbiology

1 reference

https://www.caister.com/stressresponse

Leishmania infantum (JPCM5) Transcriptome, Gene Models and Resources for an Active Curation of Gene Annotations

1 reference

Refinement of Leishmania donovani Genome Annotations in the Light of Ribosome-Protected mRNAs Fragments (Ribo-Seq Data)

37107624

1 reference

Datasets of Iso-Seq transcripts for decoding transcriptome complexity in four Leishmania species

37628688

1 reference

Protozoan parasites in the multi-omics era: present and future

38076479

1 reference

Exploring the functionality and conservation of Alba proteins in Trypanosoma cruzi: A focus on biological diversity and RNA binding ability

37780851

1 reference

A Proteogenomic Approach to Unravel New Proteins Encoded in the Leishmania donovani (HU3) Genome

38810850

1 reference

Genetic Mechanisms Involved in Microbial Stress Responses

38927711

1 reference

Section Editor-in-Chief of Genes (Microbial Genetics and Genomics Section) (English)

1 reference

https://www.mdpi.com/journal/genes/sectioneditors/MGG

external data available at URL

https://www.uam.es/uam/investigacion/grupos-de-investigacion/detalle/f1-198

0 references

https://www.cbm.uam.es/jmrequena

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interested in

regulation of gene expression

1 reference

Leishmania

1 reference

genomics

1 reference

transcriptome

1 reference

heat shock proteins

1 reference

Trypanosoma cruzi

1 reference

histone

1 reference

DNA sequencing

1 reference

molecular cloning

1 reference

proteomics

1 reference

Leishmania infantum JPCM5

1 reference

https://www.mdpi.com/2073-4425/14/4/866

Leishmania donovani HU3

1 reference

number of viewers/listeners

https://rdcu.be/bxaEi

289,595

YouTube channel ID

UCPtuIu6yCK0oabiAx1KUb8Q

YouTube handle

josemrequena4017

point in time

28 November 2025

determination method or standard

1 reference

28 November 2025

http://leish-esp.cbm.uam.es

0 references