Ubiquitin fusion technique and related methods (Q46126283)

19 December 2017

title

Ubiquitin fusion technique and related methods (English)

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19 December 2017

author name string

Alexander Varshavsky

series ordinal

1

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19 December 2017

publication date

1 January 2005

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19 December 2017

language of work or name

English

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published in

Methods in Enzymology

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19 December 2017

volume

399

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19 December 2017

page(s)

777-799

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19 December 2017

Ubiquitylation of nascent globin chains in a cell-free system

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Tackling an essential problem in functional proteomics of Saccharomyces cerevisiae

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The degradation signal in a short-lived protein

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Protein expression using ubiquitin fusion and cleavage

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Using deubiquitylating enzymes as research tools

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The Proteasome: Paradigm of a Self-Compartmentalizing Protease

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N-Terminal processing: the methionine aminopeptidase and Nα-acetyl transferase families

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7 January 2021

Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli

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Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein

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Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast

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An efficient system for high-level expression and easy purification of authentic recombinant proteins

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A multiubiquitin chain is confined to specific lysine in a targeted short-lived protein

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Probing the proteasome pathway

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Green fluorescent proteins with short half-lives as reporters in Dictyostelium discoideum

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7 January 2021

Heat-inducible degron and the making of conditional mutants

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7 January 2021

Heat-inducible degron: a method for constructing temperature-sensitive mutants.

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7 January 2021

Pairs of dipeptides synergistically activate the binding of substrate by ubiquitin ligase through dissociation of its autoinhibitory domain

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7 January 2021

Detection of a conformational change in G gamma upon binding G beta in living cells

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7 January 2021

Detection of transient in vivo interactions between substrate and transporter during protein translocation into the endoplasmic reticulum

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7 January 2021

Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome

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7 January 2021

Modulation of the intracellular stability and toxicity of diphtheria toxin through degradation by the N-end rule pathway

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7 January 2021

Toxins that are activated by HIV type-1 protease through removal of a signal for degradation by the N-end-rule pathway

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7 January 2021

The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis

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7 January 2021

The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses

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7 January 2021

Beta-lactamase protein fragment complementation assays as in vivo and in vitro sensors of protein protein interactions

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7 January 2021

A 'distributed degron' allows regulated entry into the ER degradation pathway

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7 January 2021

Characterization of the Phe-81 and Val-82 Human Fibroblast Collagenase Catalytic Domain Purified from Escherichia coli

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7 January 2021

Cdc48p interacts with Ufd3p, a WD repeat protein required for ubiquitin-mediated proteolysis in Saccharomyces cerevisiae.

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7 January 2021

A ubiquitin-specific protease that efficiently cleaves the ubiquitin-proline bond.

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7 January 2021

Tyrosinase production in recombinant E. coli containing trp promoter and ubiquitin sequence

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7 January 2021

Characterization of an essential Orc2p-associated factor that plays a role in DNA replication

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7 January 2021

Chemistry-based functional proteomics: mechanism-based activity-profiling tools for ubiquitin and ubiquitin-like specific proteases

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7 January 2021

Basic Medical Research Award. The ubiquitin system

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7 January 2021

Regulation of Membrane Protein Transport by Ubiquitin and Ubiquitin-Binding Proteins

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7 January 2021

Crystal structure of a ubiquitin-dependent degradation substrate: a three-disulfide form of lysozyme

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7 January 2021

Use of ubiquitin fusions to augment protein expression in transgenic plants

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7 January 2021

A proteolytic pathway that recognizes ubiquitin as a degradation signal

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7 January 2021

A split-ubiquitin-based assay detects the influence of mutations on the conformational stability of the p53 DNA binding domain in vivo

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7 January 2021

Split ubiquitin as a sensor of protein interactions in vivo

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7 January 2021

Ubiquitin-assisted dissection of protein transport across membranes.

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7 January 2021

Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo.

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7 January 2021

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

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7 January 2021

Cell cycle-dependent phosphorylation of the DNA polymerase epsilon subunit, Dpb2, by the Cdc28 cyclin-dependent protein kinase

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7 January 2021

A novel ubiquitination factor, E4, is involved in multiubiquitin chain assembly.

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7 January 2021

Augmentation of protein production by a combination of the T7 RNA polymerase system and ubiquitin fusion: overproduction of the human DNA repair protein, ERCC1, as a ubiquitin fusion protein in Escherichia coli.

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7 January 2021

An essential role of N-terminal arginylation in cardiovascular development

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7 January 2021

Female Lethality and Apoptosis of Spermatocytes in Mice Lacking the UBR2 Ubiquitin Ligase of the N-End Rule Pathway

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7 January 2021

Uninterrupted MCM2-7 function required for DNA replication fork progression

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7 January 2021

A new screen for protein interactions reveals that the Saccharomyces cerevisiae high mobility group proteins Nhp6A/B are involved in the regulation of the GAL1 promoter

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7 January 2021

Using ubiquitin to follow the metabolic fate of a protein

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7 January 2021

Analysis of a conditional degradation signal in yeast and mammalian cells

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7 January 2021

A ubiquitin-based assay for the cytosolic uptake of protein transduction domains

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7 January 2021

TBP-associated factors are not generally required for transcriptional activation in yeast

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7 January 2021

A strategy for the generation of conditional mutations by protein destabilization

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7 January 2021

Monitoring dynamics of gene expression in yeast during stationary phase

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7 January 2021

Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments.

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7 January 2021

Function and regulation of cullin-RING ubiquitin ligases

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7 January 2021

Back to the future with ubiquitin

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7 January 2021

Proteasomes and their kin: proteases in the machine age

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7 January 2021

Polyubiquitin chains: polymeric protein signals

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7 January 2021

Ubiquitin Fusion Technology: Bioprocessing of Peptides

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7 January 2021

Fusion proteins with COOH-terminal ubiquitin are stable and maintain dual functionality in vivo

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7 January 2021

Degradation of a cohesin subunit by the N-end rule pathway is essential for chromosome stability

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7 January 2021

Detection of altered protein conformations in living cells

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7 January 2021

Mobilizing the proteolytic machine: cell biological roles of proteasome activators and inhibitors

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7 January 2021

Identification of the long ubiquitin extension as ribosomal protein S27a

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7 January 2021

The major substrates for TAP in vivo are derived from newly synthesized proteins

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7 January 2021

Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays

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7 January 2021

Synthesis of human parathyroid-hormone-related protein(1-141) in Saccharomyces cerevisiae. A correct amino-terminal processing vital for the hormone's biological activity is obtained by an ubiquitin fusion protein approach

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7 January 2021

https://api.crossref.org/works/10.1016%2FS0076-6879%2805%2999051-4

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7 January 2021

Dissection of c-MOS degron

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7 January 2021

A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo

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7 January 2021

Degradation signals in the lysine-asparagine sequence space

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7 January 2021

Construction of tumor-specific toxins using ubiquitin fusion technique.

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7 January 2021

The N-end rule in bacteria.

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7 January 2021

Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen.

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7 January 2021

Peptides accelerate their uptake by activating a ubiquitin-dependent proteolytic pathway

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7 January 2021

Detecting and measuring cotranslational protein degradation in vivo

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7 January 2021

The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo.

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7 January 2021

Naming a targeting signal

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7 January 2021

Codominance and toxins: a path to drugs of nearly unlimited selectivity

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7 January 2021

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The N-end rule: functions, mysteries, uses

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7 January 2021

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7 January 2021

Codominant interference, antieffectors, and multitarget drugs

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7 January 2021

Ubiquitin fusion technique and its descendants

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7 January 2021

The N-end rule and regulation of apoptosis

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7 January 2021

Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome

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7 January 2021

Protein translocation across the endoplasmic reticulum

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7 January 2021

Role of DNA replication proteins in double-strand break-induced recombination in Saccharomyces cerevisiae

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7 January 2021

Ubiquitination and deubiquitination: targeting of proteins for degradation by the proteasome

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7 January 2021

Recognition of a subset of signal sequences by Ssh1p, a Sec61p-related protein in the membrane of endoplasmic reticulum of yeast Saccharomyces cerevisiae

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7 January 2021

Sec62p, a component of the endoplasmic reticulum protein translocation machinery, contains multiple binding sites for the Sec-complex

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7 January 2021

Probing the molecular environment of membrane proteins in vivo

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7 January 2021

An N-End Rule Destabilization Mutant Reveals Pre-Golgi Requirements for Sec7p in Yeast Membrane Traffic

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7 January 2021

Engineering in vivo instability of firefly luciferase and Escherichia coli beta-glucuronidase in higher plants using recognition elements from the ubiquitin pathway.

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7 January 2021

RECQL4, mutated in the Rothmund-Thomson and RAPADILINO syndromes, interacts with ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway

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7 January 2021

Combinatorial marking of cells and organelles with reconstituted fluorescent proteins

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7 January 2021

Protein-protein interactions: methods for detection and analysis

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7 January 2021

10.1016/S0076-6879(05)99051-4

Identifiers

DOI

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19 December 2017

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24 November 2022

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19 December 2017