Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function (Q36317552)

13 August 2017

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function (English)

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13 August 2017

G Dalbadie-McFarland

1

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13 August 2017

L W Cohen

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13 August 2017

A D Riggs

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13 August 2017

C Morin

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13 August 2017

K Itakura

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13 August 2017

J H Richards

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13 August 2017

1 November 1982

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Proceedings of the National Academy of Sciences of the United States of America

13 August 2017

language of work or name

English

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published in

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13 August 2017

79

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13 August 2017

21

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13 August 2017

6409-6413

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Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support

13 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Gap misrepair mutagenesis: efficient site-directed induction of transition, transversion, and frameshift mutations in vitro

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Rapid and efficient cosmid cloning

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Segment-directed mutagenesis: construction in vitro of point mutations limited to a small predetermined region of a circular DNA molecule

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

57 Sequencing end-labeled DNA with base-specific chemical cleavages

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

The structure of β-lactamases

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

A complementation analysis of the restriction and modification of DNA in Escherichia coli

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Detection of specific sequences among DNA fragments separated by gel electrophoresis

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Directed selective pressure on a beta-lactamase to analyse molecular changes involved in development of enzyme function

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Site-directed mutagenesis in DNA: generation of point mutations in cloned beta globin complementary dna at the positions corresponding to amino acids 121 to 123

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Mutagenesis at a specific position in a DNA sequence

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Efficient correction of a mutation by use of chemically synthesized DNA

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

DNA sequencing with chain-terminating inhibitors

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Penicillinase active sites: Labelling of serine-44 in β-lactamase I by 6β-bromopenicillanic acid

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

6 β-Bromopenicillanic acid inactivates β-lactamase I

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

6-beta-bromopenicillanic acid, a potent beta-lactamase inhibitor

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Site-specific mutagenesis using synthetic oligodeoxyribonucleotide primers: II. In vitro selection of mutant DNA

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Defined transversion mutations at a specific position in DNA using synthetic oligodeoxyribonucleotides as mutagens

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Filter replicas and permanent collections of recombinant DNA plasmids

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Inactivation of Bacillus cereus beta-lactamase I by 6 beta-bromopencillanic acid: mechanism

30 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

Secretion of beta-lactamase requires the carboxy end of the protein

22 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=347135

beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin

22 September 2018

1 reference

12 December 2020

Inactivation of Bacillus cereus .beta.-lactamase I by 6.beta.-bromopenicillanic acid: kinetics

1 reference

12 December 2020

Directed deletion of a yeast transfer RNA intervening sequence

1 reference

12 December 2020

Rapid preparation of covalently closed circular DNA by acridine yellow affinity chromatography

1 reference

12 December 2020

Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent phi X174 mutant, with a specific nucleotide deletion, at very high efficiency

1 reference

12 December 2020

Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase

1 reference

12 December 2020

Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site

1 reference

12 December 2020

Identifiers

DOI

10.1073/PNAS.79.21.6409

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13 August 2017

0 references

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13 August 2017

PubMed publication ID

6983070

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